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Product Info Summary
| SKU: | M01047-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-CD79a Monoclonal Antibody
SKU/Catalog Number
M01047-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-CD79a Monoclonal Antibody catalog # M01047-1. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD79a Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01047-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
ADOO-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human CD79a Antigen binding precedes formation of the CD79A and CD79B heterodimer and subsequent activation of receptor associated kinases. CD79A is a marker for B-lineage lymphoblastic leukemia; mutations in the corresponding mb-1 gene are responsible for abnormally low levels of functional B cell receptors in some cases of chronic B-cell lymphocytic leukemia.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01047-1 is reactive to CD79A in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
17 kDa
Calculated molecular weight
34061 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01047-1 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:5000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:30
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.
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Western blot analysis of CD79a expression in human spleen lysate.
Protein Target Info & Infographic
Gene/Protein Information For CD79A (Source: Uniprot.org, NCBI)
Gene Name
CD79A
Full Name
B-cell antigen receptor complex-associated protein alpha chain
Weight
34061 MW
Alternative Names
Caspase-7;CASP-7;3.4.22.60;Apoptotic protease Mch-3;Cysteine protease LICE2;Caspase-7 subunit p20;Caspase-7 subunit p11;Casp7;Lice2, Mch3; CD79A IGA, MB-1 CD79a molecule B-cell receptor complex-associated protein alpha chain|CD79a (immunoglobulin-associated alpha)|CD79a molecule, immunoglobulin-associated alpha|MB-1 membrane glycoprotein|ig-alpha|membrane-bound immunoglobulin-associated protein|surface IgM-associated protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD79A, check out the CD79A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD79A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD79a Monoclonal Antibody (M01047-1)
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No publications found for M01047-1
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Customer Q&As
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15 Customer Q&As for Anti-CD79a Monoclonal Antibody
Question
Does M01047-1 anti-CD79a Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
It shows on the product datasheet, M01047-1 anti-CD79a Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-07
Question
Is this M01047-1 anti-CD79a Monoclonal antibody reactive to the isotypes of CD79A?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
The immunogen of M01047-1 anti-CD79a Monoclonal antibody is A synthesized peptide derived from human CD79a antigen binding precedes formation of the CD79A and CD79B heterodimer and subsequent activation of receptor associated kinases. CD79A is a marker for B-lineage lymphoblastic leukemia; mutations in the corresponding mb-1 gene are responsible for abnormally low levels of functional B cell receptors in some cases of chronic B-cell lymphocytic leukemia. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-28
Question
I see that the anti-CD79a Monoclonal antibody M01047-1 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-30
Question
We are currently using anti-CD79a Monoclonal antibody M01047-1 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
The anti-CD79a Monoclonal antibody (M01047-1) has not been tested for cross reactivity specifically with dog tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-03
Question
My boss were well pleased with the WB result of your anti-CD79a Monoclonal antibody. However we have seen positive staining in adrenal gland cell membrane using this antibody. Is that expected? Could you tell me where is CD79A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
From what I have seen in literature, adrenal gland does express CD79A. Generally CD79A expresses in cell membrane. Regarding which tissues have CD79A expression, here are a few articles citing expression in various tissues:
Small intestine, Pubmed ID: 7514267
Tonsil, Pubmed ID: 1534761
Boster Scientific Support
Answered: 2019-08-01
Question
Would anti-CD79a Monoclonal antibody M01047-1 work for IF with small intestine?
Verified Customer
Verified customer
Asked: 2019-07-25
Answer
According to the expression profile of small intestine, CD79A is highly expressed in small intestine. So, it is likely that anti-CD79a Monoclonal antibody M01047-1 will work for IF with small intestine.
Boster Scientific Support
Answered: 2019-07-25
Question
Can you help my question with product M01047-1, anti-CD79a Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M01047-1 anti-CD79a Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-05
Question
I was wanting to use your anti-CD79a Monoclonal antibody for IF for human small intestine on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human small intestine identification?
Verified Customer
Verified customer
Asked: 2018-10-04
Answer
As indicated on the product datasheet, M01047-1 anti-CD79a Monoclonal antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human small intestine in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-10-04
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for small intestine using anti-CD79a Monoclonal antibody M01047-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-05-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-22
Question
Here is the WB image, lot number and protocol we used for small intestine using anti-CD79a Monoclonal antibody M01047-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-05-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-22
Question
I am looking for to test anti-CD79a Monoclonal antibody M01047-1 on human small intestine for research purposes, then I may be interested in using anti-CD79a Monoclonal antibody M01047-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
P. Patel
Verified customer
Asked: 2017-09-25
Answer
The products we sell, including anti-CD79a Monoclonal antibody M01047-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-09-25
Question
We purchased anti-CD79a Monoclonal antibody for WB on small intestine in the past. I am using human, and We are going to use the antibody for IF next. I am interested in examining small intestine as well as adrenal gland in our next experiment. Could you please give me some suggestion on which antibody would work the best for IF?
J. Jones
Verified customer
Asked: 2017-07-06
Answer
I viewed the website and datasheets of our anti-CD79a Monoclonal antibody and I see that M01047-1 has been tested on human in both WB and IF. Thus M01047-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-07-06
Question
Is a blocking peptide available for product anti-CD79a Monoclonal antibody (M01047-1)?
Z. Patel
Verified customer
Asked: 2016-03-22
Answer
We do provide the blocking peptide for product anti-CD79a Monoclonal antibody (M01047-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-03-22
Question
Is there a BSA free version of anti-CD79a Monoclonal antibody M01047-1 available?
B. Patel
Verified customer
Asked: 2015-05-18
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD79a Monoclonal antibody M01047-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-05-18
Question
We have been able to see staining in human adrenal gland. Any tips? Is anti-CD79a Monoclonal antibody supposed to stain adrenal gland positively?
F. Miller
Verified customer
Asked: 2013-02-07
Answer
Based on literature adrenal gland does express CD79A. Based on Uniprot.org, CD79A is expressed in adrenal gland, tonsil, small intestine, among other tissues. Regarding which tissues have CD79A expression, here are a few articles citing expression in various tissues:
Small intestine, Pubmed ID: 7514267
Tonsil, Pubmed ID: 1534761
Boster Scientific Support
Answered: 2013-02-07
