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Product Info Summary
| SKU: | PB9567 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-BAFF/TNFSF13B Antibody Picoband®
View all BAFF/BLyS/TNFSF13B Antibodies
SKU/Catalog Number
PB9567
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BAFF/TNFSF13B Antibody Picoband® catalog # PB9567. Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BAFF/TNFSF13B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9567)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human BAFF recombinant protein (Position: A134-L285). Human BAFF shares 86.1% amino acid (aa) sequence identity with mouse BAFF.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9567 is reactive to TNFSF13B in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
31 kDa
Calculated molecular weight
31223 MW
Background of BAFF/BLyS/TNFSF13B
BAFF was regularly detected by enzyme-linked immunosorbent assay in brain tissue lysates and in normal spinal fluid, and in astrocytes by double fluorescence microscopy. BAFF was localized in astrocytes close to BAFF-R-expressing immune cells. BAFF receptors were strongly expressed in situ in primary central nervous system (CNS) lymphomas. The TNF superfamily member B cell-activating factor (BAFF) plays an important role in humoral immunity and in autoimmune diseases, including RA.Local BAFF gene targeting inhibited proinflammatory cytokine expression, suppressed generation of plasma cells and Th17 cells, and markedly ameliorated joint pathology. The B cell activating factor BAFF (BlyS/TALL-1/zTNF4) is a tumor necrosis factor (TNF)-related ligand that promotes B cell survival and binds to three receptors (BCMA, TACI, and the recently described BAFF-R). Human BAFF was mapped to chromosome 13q32-34. The standard used in this kit is recombinant soluble human BAFF (A134-L295) with the molecular mass of 19.6KDa.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9567 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: Rat Kidney Tissue, Rat Spleen Tissue, Mouse Spleen Tissue, Human Placenta Tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of BAFF using anti-BAFF antibody (PB9567).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Rat Kidney Tissue Lysate at 50ug,
Lane 2: Rat Spleen Tissue Lysate at 50ug,
Lane 3: Mouse Spleen Tissue Lysate at 50ug,
Lane 4: Human Placenta Tissue Lysate at 50ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAFF antigen affinity purified polyclonal antibody (Catalog # PB9567) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BAFF at approximately 31 kDa. The expected band size for BAFF is at 31 kDa.
Protein Target Info & Infographic
Gene/Protein Information For TNFSF13B (Source: Uniprot.org, NCBI)
Gene Name
TNFSF13B
Full Name
Tumor necrosis factor ligand superfamily member 13B
Weight
31223 MW
Superfamily
tumor necrosis factor family
Alternative Names
Tumor necrosis factor ligand superfamily member 13B;B lymphocyte stimulator;BLyS;B-cell-activating factor;BAFF;Dendritic cell-derived TNF-like molecule;TNF- and APOL-related leukocyte expressed ligand 1;TALL-1;CD257;Tumor necrosis factor ligand superfamily member 13b, membrane form;Tumor necrosis factor ligand superfamily member 13b, soluble form;TNFSF13B;BAFF, BLYS, TALL1, TNFSF20, ZTNF4;UNQ401/PRO738; TNFSF13B BAFF, BLYS, CD257, DTL, TALL-1, TALL1, THANK, TNFSF20, TNLG7A, ZTNF4 TNF superfamily member 13b tumor necrosis factor ligand superfamily member 13B|ApoL related ligand TALL-1|B-cell-activating factor|B-lymphocyte stimulator|Delta4 BAFF|TNF and ApoL-related leukocyte expressed ligand 1|TNF homolog that activates apoptosis|delta BAFF|dendritic cell-derived TNF-like molecule|epididymis secretory sperm binding protein|tumor necrosis factor (ligand) superfamily, member 13b|tumor necrosis factor (ligand) superfamily, member 20|tumor necrosis factor ligand 7A|tumor necrosis factor superfamily member 13b|tumor necrosis factor-like protein ZTNF4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNFSF13B, check out the TNFSF13B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNFSF13B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BAFF/TNFSF13B Antibody Picoband® (PB9567)
Hello CJ!
PB9567 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Expression of B-cell activating factor in acute lymphoblastic leukemia patients
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Customer Q&As
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1 Customer Q&As for Anti-BAFF/TNFSF13B Antibody Picoband®
Question
We are currently using anti-BAFF/TNFSF13B antibody PB9567 for mouse tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-24
Answer
The anti-BAFF/TNFSF13B antibody (PB9567) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-24
