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Product Info Summary
| SKU: | M00885-2 |
|---|---|
| Size: | 100uL |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-CD34 (C-term) Rabbit Monoclonal Antibody, Clone#RM300
SKU/Catalog Number
M00885-2
Size
100uL
Form
Liquid
Description
Boster Bio Anti-CD34 (C-term) Rabbit Monoclonal Antibody, Clone#RM300 (Catalog # M00885-2). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat .
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD34 (C-term) Rabbit Monoclonal Antibody, Clone#RM300 (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00885-2)
Host
Rabbit
Contents
50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Clonality
Monoclonal
Clone Number
RM300
Isotype
Rabbit IgG
Immunogen
A peptide corresponding to the C-terminus of human CD34
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
This antibody reacts to human, mouse and rat CD34.
Reactive Species
M00885-2 is reactive to CD34 in Human, Mouse, Rat
Applications
M00885-2 is guaranteed for IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
40716 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (IHC): 1:100-1:200 dilution
WB: 1:100-1:200 dilution.
Validation Images & Assay Conditions
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Figure 1. Western Blotting result
Western Blot of mouse spleen tissue lysate using anti-CD34 rabbit monoclonal antibody (Clone RM300) at a 1:100 dilution.
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Figure 2. IHC result
Immunohistochemical staining of formalin fixed and paraffin embedded human kidney tissue section using anti-CD34 rabbit monoclonal antibody (Clone RM300) at a 1:200 dilution.
Protein Target Info & Infographic
Gene/Protein Information For CD34 (Source: Uniprot.org, NCBI)
Gene Name
CD34
Full Name
Hematopoietic progenitor cell antigen CD34
Weight
40716 MW
Superfamily
CD34 family
Alternative Names
CD34 antigenhematopoietic progenitor cell antigen CD34; CD34 molecule; CD34; HPCA1 CD34 CD34 molecule hematopoietic progenitor cell antigen CD34|CD34 antigen
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD34, check out the CD34 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD34: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD34 (C-term) Rabbit Monoclonal Antibody, Clone#RM300 (M00885-2)
Hello CJ!
M00885-2 has been cited in 25 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Assefa F,Lim J,Kim JA,Ihn HJ,Lim S,Nam SH,Bae YC,Park EK. Secretoneurin, a Neuropeptide, Enhances Bone Regeneration in a Mouse Calvarial Bone Defect Model. Tissue Eng Regen Med.2020 Nov 3.doi: 10.1007/s13770-020-00304-1.Epub ahead of print.PMID:33145742.
Species: Mouse
M00885-2 usage in article: APP:IHC, SAMPLE:CALVARIAL BONE, DILUTION:1:500
Dl-3-n-Butylphthalide treatment enhances hemodynamics and ameliorates memory deficits in rats with chronic cerebral hypoperfusion
Arginine ADP-ribosyltransferase 1 promotes angiogenesis in colorectal cancer via the PI3K/Akt pathway
Effects of human umbilical cord mesenchymal stem cell transplantation combined with minimally invasive hematoma aspiration on intracerebral hemorrhage in rats.
Local recurrence of small cell lung cancer following radiofrequency ablation is induced by HIF-1? expression in the transition zone
Heparin Impairs Angiogenesis through Inhibition of MicroRNA-10b
Hyperthermia induced HIF-1a expression of lung cancer through AKT and ERK signaling pathways
Incomplete radiofrequency ablation accelerates proliferation and angiogenesis of residual lung carcinomas via HSP70/HIF-1?
Buyanghuanwu decoction promotes angiogenesis after cerebral ischemia/reperfusion injury: mechanisms of brain tissue repair
Radix Ilicis Pubescentis total flavonoids combined with mobilization of bone marrow stem cells to protect against cerebral ischemia/reperfusion injury
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Customer Reviews
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Customer Q&As
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16 Customer Q&As for Anti-CD34 (C-term) Rabbit Monoclonal Antibody, Clone#RM300
Question
Our lab were content with the WB result of your anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone
Verified Customer
Verified customer
Asked: 2020-03-31
Answer
From what I have seen in literature, bone marrow does express CD34. Generally CD34 expresses in membrane. Regarding which tissues have CD34 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 1370171
Placenta, Pubmed ID: 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-03-31
Question
I was wanting to use to test anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 M00885-2 on human megakaryoblast for research purposes, then I may be interested in using anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 M00885-2 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
The products we sell, including anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 M00885-2, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-03-11
Question
We have been able to see staining in human bone marrow. Are there any suggestions? Is anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 supposed to stain bone marrow positively?
Verified Customer
Verified customer
Asked: 2020-01-31
Answer
From what I have seen in literature bone marrow does express CD34. From what I have seen in Uniprot.org, CD34 is expressed in esophagus, bone marrow, megakaryoblast, heart, placenta, skin, among other tissues. Regarding which tissues have CD34 expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 1370171
Placenta, Pubmed ID: 14702039
Skin, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2020-01-31
Question
I am interested in using your anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 for metanephric glomerular mesangial cell differentiation studies. Has this antibody been tested with western blotting on human kidney tissue? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-01-24
Answer
We appreciate your inquiry. This M00885-2 anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 is tested on mouse spleen tissue, tissue lysate, human kidney tissue. It is guaranteed to work for IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-01-24
Question
See attached the WB image, lot number and protocol we used for megakaryoblast using anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 M00885-2. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-08-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-06
Question
I was wanting to use your anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 for WB for human megakaryoblast on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human megakaryoblast identification?
Verified Customer
Verified customer
Asked: 2019-07-29
Answer
It shows on the product datasheet, M00885-2 anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 has been validated for IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human megakaryoblast in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-29
Question
We are currently using anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 M00885-2 for human tissue, and we are happy with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
The anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 (M00885-2) has not been validated for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-23
Question
I see that the anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 M00885-2 works with WB, what is the protocol used to produce the result images on the product page?
G. Jones
Verified customer
Asked: 2018-06-13
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-06-13
Question
I have a question about product M00885-2, anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
K. Li
Verified customer
Asked: 2018-01-23
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00885-2 anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-01-23
Question
Is this M00885-2 anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 reactive to the isotypes of CD34?
Verified Customer
Verified customer
Asked: 2018-01-10
Answer
The immunogen of M00885-2 anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 is A peptide corresponding to the C-terminus of human CD34. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-01-10
Question
Is there a BSA free version of anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 M00885-2 available?
Verified Customer
Verified customer
Asked: 2017-08-03
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 M00885-2 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-08-03
Question
Does M00885-2 anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
R. Parker
Verified customer
Asked: 2017-06-27
Answer
As indicated on the product datasheet, M00885-2 anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-27
Question
Is a blocking peptide available for product anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 (M00885-2)?
C. Krishna
Verified customer
Asked: 2017-02-13
Answer
We do provide the blocking peptide for product anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 (M00885-2). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2017-02-13
Question
Would anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 M00885-2 work for WB with megakaryoblast?
E. Mitchell
Verified customer
Asked: 2015-12-09
Answer
According to the expression profile of megakaryoblast, CD34 is highly expressed in megakaryoblast. So, it is likely that anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 M00885-2 will work for WB with megakaryoblast.
Boster Scientific Support
Answered: 2015-12-09
Question
We ordered your anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone
B. Roberts
Verified customer
Asked: 2014-07-03
Answer
I took a look at the website and datasheets of our anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone
Boster Scientific Support
Answered: 2014-07-03
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for megakaryoblast using anti-CD34 (C-term) Rabbit Monoclonal antibody, Clone#RM300 M00885-2. Let me know if you need anything else.
J. Bhatt
Verified customer
Asked: 2013-06-19
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-06-19
