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Product Info Summary
| SKU: | PB9932 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry, IHC, ICC, WB |
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Product info
Product Name
Anti-CD33 Antibody Picoband®
View all Siglec-3/CD33 Antibodies
SKU/Catalog Number
PB9932
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD33 Antibody Picoband® catalog # PB9932. Tested in Flow Cytometry, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD33 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9932)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CD33 recombinant protein (Position: D18-H259). Human CD33 shares 61.6% amino acid (aa) sequence identity with mouse CD33.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9932 is reactive to CD33 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
39825 MW
Background of Siglec-3/CD33
CD33, also known as Siglec-3 (sialic acid binding Ig-like lectin 3, SIGLEC3, SIGLEC-3, gp67, p67), is a transmembrane receptor expressed on cells of myeloid lineage. It is usually considered myeloid-specific, but it can also be found on some lymphoid cells. CD33 binds sialic acids, therefore is a member of the SIGLEC family of lectins. By fluorescence in situ hybridization, CD33 is mapped to 19q13.3-q13.4.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9932 is guaranteed for Flow Cytometry, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Immunohistochemistry (Frozen Section), 0.5-1μg/ml
Immunocytochemistry, 0.5-1μg/ml
Western blot, 0.1-0.5μg/ml
Flow Cytometry, 1-3μg/1x106cells
Positive Control
WB: SKOV3 whole cell
IHC: human tonsil tissue
FCM: U937 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD33 using anti-CD33 antibody (PB9932).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: SKOV3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD33 antigen affinity purified polyclonal antibody (Catalog # PB9932) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD33 at approximately 45 kDa. The expected band size for CD33 is at 40 kDa.
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Figure 2. IHC analysis of CD33 using anti-CD33 antibody (PB9932).
CD33 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-CD33 Antibody (PB9932) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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3. Flow Cytometry analysis of U937 cells using anti-CD33 antibody (PB9932).Overlay histogram showing U937 cells stained with PB9932 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD33 Antibody (PB9932,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For CD33 (Source: Uniprot.org, NCBI)
Gene Name
CD33
Full Name
Myeloid cell surface antigen CD33
Weight
39825 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Myeloid cell surface antigen CD33;Sialic acid-binding Ig-like lectin 3;Siglec-3;gp67;CD33;CD33;SIGLEC3; CD33 SIGLEC-3, SIGLEC3, p67 CD33 molecule myeloid cell surface CD33|CD33 (gp67)|CD33 molecule transcript|gp67|sialic acid-binding Ig-like lectin 3
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD33, check out the CD33 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD33: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD33 Antibody Picoband® (PB9932)
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Customer Q&As
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16 Customer Q&As for Anti-CD33 Antibody Picoband®
Question
I am interested in using your anti-CD33 antibody for neutrophil degranulation studies. Has this antibody been tested with western blotting on u937 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2020-04-15
Answer
Thank you for your inquiry. This PB9932 anti-CD33 antibody is tested on skov3 whole cell lysates, u937 cells. It is guaranteed to work for Flow Cytometry, IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2020-04-15
Question
We have been able to see staining in human uterus. Are there any suggestions? Is anti-CD33 antibody supposed to stain uterus positively?
Verified Customer
Verified customer
Asked: 2020-03-18
Answer
From literature uterus does express CD33. From Uniprot.org, CD33 is expressed in leukocyte, premonocytic lymphoma, uterus, among other tissues. Regarding which tissues have CD33 expression, here are a few articles citing expression in various tissues:
Leukocyte, Pubmed ID: 15489334
Premonocytic lymphoma, Pubmed ID: 3139766, 11943481
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2020-03-18
Question
I see that the anti-CD33 antibody PB9932 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-03-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-03-17
Question
I have a question about product PB9932, anti-CD33 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-01-10
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9932 anti-CD33 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-01-10
Question
Does anti-CD33 antibody PB9932 work for WB with leukocyte?
Verified Customer
Verified customer
Asked: 2020-01-03
Answer
According to the expression profile of leukocyte, CD33 is highly expressed in leukocyte. So, it is likely that anti-CD33 antibody PB9932 will work for WB with leukocyte.
Boster Scientific Support
Answered: 2020-01-03
Question
I was wanting to use your anti-CD33 antibody for WB for human leukocyte on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human leukocyte identification?
Verified Customer
Verified customer
Asked: 2019-10-23
Answer
As indicated on the product datasheet, PB9932 anti-CD33 antibody has been tested for Flow Cytometry, IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human leukocyte in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-10-23
Question
Is this PB9932 anti-CD33 antibody reactive to the isotypes of CD33?
W. Walker
Verified customer
Asked: 2019-09-03
Answer
The immunogen of PB9932 anti-CD33 antibody is E. coli-derived human CD33 recombinant protein (Position: D18-H259). Human CD33 shares 61.6% amino acid (aa) sequence identity with mouse CD33. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-09-03
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for leukocyte using anti-CD33 antibody PB9932. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-31
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-31
Question
I am interested in to test anti-CD33 antibody PB9932 on human leukocyte for research purposes, then I may be interested in using anti-CD33 antibody PB9932 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-24
Answer
The products we sell, including anti-CD33 antibody PB9932, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-24
Question
Does PB9932 anti-CD33 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-06-20
Answer
It shows on the product datasheet, PB9932 anti-CD33 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-06-20
Question
I have attached the WB image, lot number and protocol we used for leukocyte using anti-CD33 antibody PB9932. Please let me know if you require anything else.
R. Gonzalez
Verified customer
Asked: 2018-05-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-05-17
Question
Is a blocking peptide available for product anti-CD33 antibody (PB9932)?
Verified Customer
Verified customer
Asked: 2018-04-05
Answer
We do provide the blocking peptide for product anti-CD33 antibody (PB9932). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-04-05
Question
We are currently using anti-CD33 antibody PB9932 for human tissue, and we are well pleased with the ICC results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on goat tissues as well?
F. Roberts
Verified customer
Asked: 2017-07-25
Answer
The anti-CD33 antibody (PB9932) has not been tested for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-07-25
Question
My boss were satisfied with the WB result of your anti-CD33 antibody. However we have seen positive staining in uterus isoform cd33m: cell membrane using this antibody. Is that expected? Could you tell me where is CD33 supposed to be expressed?
D. Wu
Verified customer
Asked: 2017-03-22
Answer
From what I have seen in literature, uterus does express CD33. Generally CD33 expresses in isoform cd33m: cell membrane, isoform cd33m: peroxisome. Regarding which tissues have CD33 expression, here are a few articles citing expression in various tissues:
Leukocyte, Pubmed ID: 15489334
Premonocytic lymphoma, Pubmed ID: 3139766, 11943481
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-03-22
Question
Is there a BSA free version of anti-CD33 antibody PB9932 available?
E. Mangal
Verified customer
Asked: 2015-09-08
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD33 antibody PB9932 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2015-09-08
Question
We bought anti-CD33 antibody for Flow Cytometry on uterus in the past. I am using human, and We are going to use the antibody for ICC next. We are interested in examining uterus as well as leukocyte in our next experiment. Could you please give me some suggestion on which antibody would work the best for ICC?
M. Rodriguez
Verified customer
Asked: 2013-07-18
Answer
I viewed the website and datasheets of our anti-CD33 antibody and it seems that PB9932 has been validated on human in both Flow Cytometry and ICC. Thus PB9932 should work for your application. Our Boster satisfaction guarantee will cover this product for ICC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ICC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-07-18
