Click image to see more details
-
-
-
-
-
+1
Product Info Summary
| SKU: | A01512 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-OX-2 membrane glycoprotein CD200 Antibody
SKU/Catalog Number
A01512
Size
100μl
Form
Liquid
Description
Boster Bio Anti-OX-2 membrane glycoprotein CD200 Antibody catalog # A01512. Tested in IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-OX-2 membrane glycoprotein CD200 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01512)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from the Internal region of human CD200. AA range:171-220
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A01512 is reactive to CD200 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
31264 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01512 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB, 1:500-1:2000
IHC-P, 1:100-1:300
Validation Images & Assay Conditions
Click image to see more details
Western Blot (WB) analysis of PC12 cells using CD200 Polyclonal antibody.
Click image to see more details
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain, antibody was diluted at 1:100.
Click image to see more details
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Liver, antibody was diluted at 1:100.
Click image to see more details
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Brain, antibody was diluted at 1:100.
Click image to see more details
Immunohistochemistry (IHC) analysis of paraffin-embedded Human Liver, antibody was diluted at 1:100.
Protein Target Info & Infographic
Gene/Protein Information For CD200 (Source: Uniprot.org, NCBI)
Gene Name
CD200
Full Name
OX-2 membrane glycoprotein
Weight
31264 MW
Alternative Names
OX-2 membrane glycoprotein;CD200;CD200;MOX1, MOX2;My033; CD200 MOX1, MOX2, MRC, OX-2 CD200 molecule OX-2 membrane glycoprotein|CD200 | by MRC OX-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD200, check out the CD200 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD200: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-OX-2 membrane glycoprotein CD200 Antibody (A01512)
Hello CJ!
No publications found for A01512
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-OX-2 membrane glycoprotein CD200 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-OX-2 membrane glycoprotein CD200 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-OX-2 membrane glycoprotein CD200 Antibody
Question
Is there a BSA free version of anti-CD200 antibody A01512 available?
R. Bhatt
Verified customer
Asked: 2020-04-28
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-CD200 antibody A01512 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-28
Question
Can you help my question with product A01512, anti-CD200 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01512 anti-CD200 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-04-09
Question
I was wanting to use your anti-CD200 antibody for WB for human blood on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human blood identification?
Verified Customer
Verified customer
Asked: 2020-02-21
Answer
It shows on the product datasheet, A01512 anti-CD200 antibody has been tested for IHC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-21
Question
I see that the anti-CD200 antibody A01512 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-10-17
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-10-17
Question
My question regards to test anti-CD200 antibody A01512 on human blood for research purposes, then I may be interested in using anti-CD200 antibody A01512 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-10-16
Answer
The products we sell, including anti-CD200 antibody A01512, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-10-16
Question
Is this A01512 anti-CD200 antibody reactive to the isotypes of CD200?
S. Evans
Verified customer
Asked: 2019-10-07
Answer
The immunogen of A01512 anti-CD200 antibody is Synthesized peptide derived from the Internal region of human CD200. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-07
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-CD200 antibody A01512. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-08-29
Question
Would anti-CD200 antibody A01512 work for WB with blood?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
According to the expression profile of blood, CD200 is highly expressed in blood. So, it is likely that anti-CD200 antibody A01512 will work for WB with blood.
Boster Scientific Support
Answered: 2019-07-12
Question
See below the WB image, lot number and protocol we used for blood using anti-CD200 antibody A01512. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-16
Question
Is a blocking peptide available for product anti-CD200 antibody (A01512)?
Verified Customer
Verified customer
Asked: 2018-09-21
Answer
We do provide the blocking peptide for product anti-CD200 antibody (A01512). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-09-21
Question
We are currently using anti-CD200 antibody A01512 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on feline tissues as well?
G. Wu
Verified customer
Asked: 2018-02-28
Answer
The anti-CD200 antibody (A01512) has not been validated for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-02-28
Question
We have seen staining in human blood. Do you have any suggestions? Is anti-CD200 antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2017-12-28
Answer
From literature blood does express CD200. From Uniprot.org, CD200 is expressed in chorionic villus, fetal brain, brain, blood, among other tissues. Regarding which tissues have CD200 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3032785
Brain, Pubmed ID: 14702039, 15489334
Boster Scientific Support
Answered: 2017-12-28
Question
We want using your anti-CD200 antibody for immunoregulatory interactions between a lymphoid and a non-lymphoid cell studies. Has this antibody been tested with western blotting on pc12 cells? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-08-07
Answer
Thank you for your inquiry. This A01512 anti-CD200 antibody is validated on pc12 cells. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-08-07
Question
Will A01512 anti-CD200 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
B. Edwards
Verified customer
Asked: 2016-12-14
Answer
As indicated on the product datasheet, A01512 anti-CD200 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2016-12-14
Question
We were well pleased with the WB result of your anti-CD200 antibody. However we have seen positive staining in brain cell membrane using this antibody. Is that expected? Could you tell me where is CD200 supposed to be expressed?
L. Jha
Verified customer
Asked: 2014-08-06
Answer
From what I have seen in literature, brain does express CD200. Generally CD200 expresses in cell membrane. Regarding which tissues have CD200 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3032785
Brain, Pubmed ID: 14702039, 15489334
Boster Scientific Support
Answered: 2014-08-06
Question
We ordered your anti-CD200 antibody for WB on brain in the past. I am using human, and We want to use the antibody for IHC next. We need examining brain as well as chorionic villus in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
E. Krishna
Verified customer
Asked: 2013-07-09
Answer
I looked at the website and datasheets of our anti-CD200 antibody and it seems that A01512 has been tested on human in both WB and IHC. Thus A01512 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2013-07-09
