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Product Info Summary
| SKU: | FEK0993 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay Time: | 90 Minutes |
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Product info
Product Name
Human CD200 PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK0993
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD200 PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human CD200 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human CD200 PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK0993)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q31-G232
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK0993 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK0993 is reactive to CD200 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Application Guarantee
FEK0993 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD200/OX2
OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13.2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK0993-CAP | Anti-Human CD200/OX2 Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK0993-ST | Human CD200/OX2 Standard | 2 | 10 ng/tube |
| FEK0993-H | HRP Linked Anti-Human CD200 Antibody | 1 | 6ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.015 | 0.062 | 0.099 | 0.185 | 0.329 | 0.625 | 1.179 | 2.046 |
Data Example Images
Click image to see more details
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD200 PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 89 | 252 | 897 | 75 | 251 | 9606 |
| Standard deviation | 4.36 | 16.12 | 69.06 | 4.12 | 14.30 | 72 |
| CV (%) | 4.9% | 6.4% | 7.7% | 5.5% | 5.7% | 7.5% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD200/OX2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 89 | 75 | 93 | 98 | 88 | 4.66 | 5.3% |
| Sample 2 | 252 | 245 | 216 | 254 | 241 | 7.47 | 3.1% |
| Sample 3 | 897 | 862 | 897 | 871 | 881 | 22.02 | 2.5% |
Protein Target Info & Infographic
Gene/Protein Information For CD200 (Source: Uniprot.Org, NCBI)
Gene Name
CD200
Full Name
OX-2 membrane glycoprotein
Weight
31.264kDa
Alternative Names
antigen identified by monoclonal MRC OX-2; CD200 antigenMOX1; CD200 molecule; CD200; MOX1; MOX2; MOX2MRC; MRC OX-2 antigen; MRC; OX-2 membrane glycoprotein; OX-2 CD200 MOX1, MOX2, MRC, OX-2 CD200 molecule OX-2 membrane glycoprotein|CD200 | by MRC OX-2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD200, check out the CD200 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD200: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD200 PicoKine® Quick ELISA Kit (FEK0993)
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