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Product Info Summary
| SKU: | A01512-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-CD200/OX2 Antibody Picoband®
SKU/Catalog Number
A01512-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD200/OX2 Antibody Picoband® catalog # A01512-1. Tested in ELISA, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD200/OX2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01512-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CD200/OX2 recombinant protein (Position: Q31-K231).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01512-1 is reactive to CD200 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
31.264kDa
Background of CD200/OX2
OX-2 membrane glycoprotein, also named CD200 (Cluster of Differentiation 200) is a human protein encoded by the CD200 gene. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. Multiple alternatively spliced transcript variants that encode different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01512-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human Hela whole cell, rat brain tissue, mouse brain tissue, mouse SP20 whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of CD200/OX2 using anti-CD200/OX2 antibody (A01512-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: rat brain tissue lysates,
Lane 3: mouse brain tissue lysates,
Lane 4: mouse SP20 whole cell lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD200/OX2 antigen affinity purified polyclonal antibody (Catalog # A01512-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD200/OX2 at approximately 45KD. The expected band size for CD200/OX2 is at 31KD.
Protein Target Info & Infographic
Gene/Protein Information For CD200 (Source: Uniprot.org, NCBI)
Gene Name
CD200
Full Name
OX-2 membrane glycoprotein
Weight
31.264kDa
Alternative Names
OX-2 membrane glycoprotein; CD200; MOX1; MOX2; My033 CD200 MOX1, MOX2, MRC, OX-2 CD200 molecule OX-2 membrane glycoprotein|CD200 | by MRC OX-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CD200, check out the CD200 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CD200: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD200/OX2 Antibody Picoband® (A01512-1)
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Customer Q&As
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5 Customer Q&As for Anti-CD200/OX2 Antibody Picoband®
Question
We are currently using anti-CD200/OX2 antibody A01512-1 for mouse tissue, and we are happy with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
The anti-CD200/OX2 antibody (A01512-1) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-09
Question
My colleagues were satisfied with the WB result of your anti-CD200/OX2 antibody. However we have seen positive staining in blood cell membrane using this antibody. Is that expected? Could you tell me where is CD200 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-28
Answer
According to literature, blood does express CD200. Generally CD200 expresses in cell membrane. Regarding which tissues have CD200 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3032785
Brain, Pubmed ID: 14702039, 15489334
Boster Scientific Support
Answered: 2020-01-28
Question
We ordered your anti-CD200/OX2 antibody for WB on fetal brain in a previous experiment. I am using human, and We are going to use the antibody for ELISA next. I am interested in examining fetal brain as well as blood in our next experiment. Could you please give me some suggestion on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2019-05-15
Answer
I looked at the website and datasheets of our anti-CD200/OX2 antibody and it appears that A01512-1 has been validated on human in both WB and ELISA. Thus A01512-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-05-15
Question
I was wanting to use using your anti-CD200/OX2 antibody for positive regulation of transforming growth factor beta production studies. Has this antibody been tested with western blotting on mouse brain? We would like to see some validation images before ordering.
L. Evans
Verified customer
Asked: 2019-02-27
Answer
Thanks for your inquiry. This A01512-1 anti-CD200/OX2 antibody is tested on human hela, hela whole cell lysates, rat brain tissue, mouse brain. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-02-27
Question
We have been able to see staining in rat blood. Do you have any suggestions? Is anti-CD200/OX2 antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2017-08-08
Answer
From what I have seen in literature blood does express CD200. From what I have seen in Uniprot.org, CD200 is expressed in chorionic villus, fetal brain, brain, blood, among other tissues. Regarding which tissues have CD200 expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 3032785
Brain, Pubmed ID: 14702039, 15489334
Boster Scientific Support
Answered: 2017-08-08
