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Product Info Summary
| SKU: | EK0993 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human CD200 ELISA Kit PicoKine®
SKU/Catalog Number
EK0993
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD200 ELISA Kit PicoKine® (96 Tests). Quantitate Human CD200 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD200 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0993)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q31-G232
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0993 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0993 is reactive to CD200 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0993 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD200/OX2
OX-2 membrane glycoprotein, also known as CD200 is a human protein encoded by the CD200 gene. This gene is mapped to 3q13.2. The protein encoded by this gene is a type-1 membrane glycoprotein, which contains two immunoglobulin domains, and thus belongs to the immunoglobulin superfamily. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. It is suggested that CD200-CD200R signaling plays a central role in macrophage fusion and osteoclast formation.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0993-CAP | Anti-Human CD200 Pre-coated 96-well strip microplate | 1 |
| EK0993-ST | Human CD200 Standard | 2 vials, 10 ng/tube |
| EK0993-DA | Human CD200 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.011 | 0.108 | 0.189 | 0.341 | 0.693 | 1.065 | 1.838 | 2.445 |
Data Example Images
Click image to see more details
Human CD200 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD200 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 80 | 246 | 894 | 73 | 239 | 980 |
| Standard deviation | 3.76 | 12.54 | 68.83 | 4.16 | 14.81 | 77.42 |
| CV (%) | 4.7% | 7.9% | 7.7% | 5.7% | 6.2% | 7.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD200/OX2 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 80 | 78 | 90 | 88 | 84 | 5.09 | 6% |
| Sample 2 | 246 | 237 | 227 | 247 | 239 | 8.07 | 3.3% |
| Sample 3 | 894 | 868 | 899 | 880 | 885 | 12.15 | 1.3% |
Protein Target Info & Infographic
Gene/Protein Information For CD200 (Source: Uniprot.Org, NCBI)
Gene Name
CD200
Full Name
OX-2 membrane glycoprotein
Weight
31264 MW
Alternative Names
OX-2 membrane glycoprotein;CD200;CD200;MOX1, MOX2;My033; CD200 MOX1, MOX2, MRC, OX-2 CD200 molecule OX-2 membrane glycoprotein|CD200 | by MRC OX-2
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on CD200, check out the CD200 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for CD200: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD200 ELISA Kit PicoKine® (EK0993)
Hello CJ!
EK0993 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
CD200/CD200 receptor axis in psoriasis vulgaris
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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11 Customer Q&As for Human CD200 ELISA Kit PicoKine®
Question
Q: can you suggest the dilution ratio of serum samples for detection of CD200 in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.
P. Martin
Verified customer
Asked: 2020-11-06
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD200 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-11-06
Question
Q: What is the optimal O.D. value for CD200 ELISA kit? I performed your CD200 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain CD200 even though the O.D. values are not very high?
T. Yang
Verified customer
Asked: 2020-10-14
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this CD200 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of CD200 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2020-10-14
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2020-07-22
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2020-07-22
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-04-21
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-04-21
Question
A customer asks for your in-house testing data for normal human serum with the kit EK0993. Would you have this information available to share?
Verified Customer
Verified customer
Asked: 2020-04-20
Answer
The concentration range of CD200 in normal healthy human serum obtained by our lab technician is 28-41pg/ml.
Boster Scientific Support
Answered: 2020-04-20
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis CD200 using the EK0993 Human CD200 Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-03-07
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2020-03-07
Question
Is there a positive control for the EK0993 kit?
Verified customer
Asked: 2019-05-06
Answer
The controls we can only provide for the Human CD200 ELISA Kit PicoKine™ (EK0993) is recombinant protein. The size is around 200pg for this control.
Boster Scientific Support
Answered: 2019-05-07
Question
What is the concentration range of CD200 in normal healthy human serum obtained from your in-house testing of EK0993?
Verified customer
Asked: 2019-04-29
Answer
For the Human CD200 ELISA Kit PicoKine™ (EK0993), the concentration range of CD200 in normal healthy human serum obtained by our lab technician is 28-41pg/ml.
Boster Scientific Support
Answered: 2019-04-30
Question
Q: Can CD200 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
G. Smith
Verified customer
Asked: 2018-02-06
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2018-02-06
Question
Q: which procedure should I follow in order to thaw whole blood sample for CD200 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-01-04
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD200 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-01-04
Question
Q: can I use citrate plasma as samples in Human CD200 Picokine® ELISA Kit (Catalog # EK0993)?
W. Smith
Verified customer
Asked: 2016-12-23
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of CD200 causing degradation of its protein structure. CD200 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the CD200 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-12-23
