Click image to see more details
Product Info Summary
| SKU: | M01408-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IF, ICC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-CD16 Rabbit Monoclonal Antibody
SKU/Catalog Number
M01408-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-CD16 Rabbit Monoclonal Antibody catalog # M01408-1. Tested in WB, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD16 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01408-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
22F02
Isotype
IgG
Immunogen
A synthesized peptide derived from human CD16
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M01408-1 is reactive to FCGR3A in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
42 kDa
Calculated molecular weight
76063 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01408-1 is guaranteed for Flow Cytometry, IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
ICC/IF 1:50-1:200
FC 1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of CD16 using anti-CD16 antibody (M01408-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: rat PC-12 whole cell lysates,
Lane 3: mouse spleen tissue lysates,
Lane 4: mouse RAW264.7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD16 antigen affinity purified monoclonal antibody (Catalog # M01408-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD16 at approximately 42 kDa. The expected band size for CD16 is at 29 kDa.
Click image to see more details
Western blot analysis of CD16 expression in THP-1 cell lysate.
Click image to see more details
All lanes use the Antibody at 1:5k dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For FCGR3A (Source: Uniprot.org, NCBI)
Gene Name
FCGR3A
Full Name
Low affinity immunoglobulin gamma Fc region receptor III-A
Weight
76063 MW
Alternative Names
RalA-binding protein 1;RalBP1;76 kDa Ral-interacting protein;Dinitrophenyl S-glutathione ATPase;DNP-SG ATPase;Ral-interacting protein 1;RALBP1;RLIP1, RLIP76; FCGR3A CD16, CD16A, FCG3, FCGR3, FCGRIII, FCR-10, FCRIII, FCRIIIA, IGFR3, IMD20 Fc fragment of IgG receptor IIIa low affinity immunoglobulin gamma Fc region receptor III-A|CD16a |Fc fragment of IgG, low affinity III, receptor for (CD16)|Fc fragment of IgG, low affinity IIIa, receptor (CD16a)|Fc gamma receptor III-A|Fc-gamma RIII-alpha|Fc-gamma receptor III-2 (CD 16)|Fc-gamma receptor IIIb (CD16)|FcgammaRIIIA|igG Fc receptor III-2|immunoglobulin G Fc receptor III|low affinity immunoglobulin gamma receptor III-a Fc fragment|neutrophil-specific NA
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FCGR3A, check out the FCGR3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FCGR3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD16 Rabbit Monoclonal Antibody (M01408-1)
Hello CJ!
M01408-1 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Fingolimod suppressed the chronic unpredictable mild stress-induced depressive-like behaviors via affecting microglial and NLRP3 inflammasome activation
IP-10 and fractalkine induce cytotoxic phenotype of murine NK cells
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-CD16 Rabbit Monoclonal Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-CD16 Rabbit Monoclonal Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
