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Product Info Summary
| SKU: | FC01408-PE |
|---|---|
| Size: | 100 tests, 200 tests |
| Reactive Species: | Cynomolgus monkey, Human, Baboon, Rhesus monkey |
| Host: | Mouse |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-human CD16 FCGR3A Monoclonal Antibody PE Conjugated, Flow Validated
SKU/Catalog Number
FC01408-PE
Size
100 tests, 200 tests
Form
Liquid
Description
Boster Bio Anti-human CD16 FCGR3A Monoclonal Antibody PE Conjugated, Flow Validated (Catalog# FC01408-PE). Tested in Flow Cytometry application(s). This antibody reacts with Human, Rhesus monkey, Cynomolgus monkey, Baboon.
Storage & Handling
Store at 2-8°C. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-human CD16 FCGR3A Monoclonal Antibody PE Conjugated, Flow Validated (Boster Biological Technology, Pleasanton CA, USA, Catalog # FC01408-PE)
Host
Mouse
Contents
PBS pH 7.2, 0.2% (w/v) BSA, 0.09% (w/v) sodium azide
Clonality
Monoclonal
Clone Number
Clone: 3G8
Isotype
IgG1,k
Immunogen
Human peripheral blood T lymphocytes
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
FC01408-PE is reactive to FCGR3A in Cynomolgus monkey, Human, Baboon, Rhesus monkey
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
29089 MW
Background of FCGR3A
SCFR (Mast/stem cell growth factor receptor), also known as proto-oncogene c-Kit or tyrosine-protein kinase Kit or CD117, is a protein that in humans is encoded by the KIT gene. KIT was first described as the cellular homolog of the feline sarcoma viral oncogene v-kit. The KIT gene is mapped on 4q12. Kit was expressed on the surface of germ cells up to the pachytene stage. Signaling from the KIT receptor tyrosine kinase is essential for primordial germ cell growth both in vivo and in vitro. Determination of the KIT effectors acting in primordial germ cells has been hampered by the lack of effective methods to manipulate easily gene expression in these cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
FC01408-PE is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry, 5μl is enough to stain 1 million PBMCs or 100μl whole blood sample
Validation Images & Assay Conditions
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Lymphocytes-gated PBMCs stained with CD16 (3G8) PE (RED); Lymphocytes-gated PBMCs stained with mouse IgG1 PE isotype control (BLUE).
Protein Target Info & Infographic
Gene/Protein Information For FCGR3A (Source: Uniprot.org, NCBI)
Gene Name
FCGR3A
Full Name
Low affinity immunoglobulin gamma Fc region receptor III-A
Weight
29089 MW
Alternative Names
Low affinity immunoglobulin gamma Fc region receptor III-A;CD16a antigen;Fc-gamma RIII-alpha;Fc-gamma RIII;Fc-gamma RIIIa;FcRIII;FcRIIIa;FcR-10;IgG Fc receptor III-2;CD16a;FCGR3A;CD16A, FCG3, FCGR3, IGFR3; FCGR3A CD16, CD16A, FCG3, FCGR3, FCGRIII, FCR-10, FCRIII, FCRIIIA, IGFR3, IMD20 Fc fragment of IgG receptor IIIa low affinity immunoglobulin gamma Fc region receptor III-A|CD16a |Fc fragment of IgG, low affinity III, receptor for (CD16)|Fc fragment of IgG, low affinity IIIa, receptor (CD16a)|Fc gamma receptor III-A|Fc-gamma RIII-alpha|Fc-gamma receptor III-2 (CD 16)|Fc-gamma receptor IIIb (CD16)|FcgammaRIIIA|igG Fc receptor III-2|immunoglobulin G Fc receptor III|low affinity immunoglobulin gamma receptor III-a Fc fragment|neutrophil-specific NA
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FCGR3A, check out the FCGR3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FCGR3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-human CD16 FCGR3A Monoclonal Antibody PE Conjugated, Flow Validated (FC01408-PE)
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No publications found for FC01408-PE
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Customer Reviews
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Customer Q&As
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11 Customer Q&As for Anti-human CD16 FCGR3A Monoclonal Antibody PE Conjugated, Flow Validated
Question
Do you have a BSA free version of anti-human CD16 Monoclonal antibody PE Conjugated, Flow Validated FC01408-PE available?
S. Baker
Verified customer
Asked: 2020-02-13
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-human CD16 Monoclonal antibody PE Conjugated, Flow Validated FC01408-PE are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-02-13
Question
Will FC01408-PE anti-human CD16 Monoclonal antibody PE Conjugated, Flow Validated work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-06
Answer
It shows on the product datasheet, FC01408-PE anti-human CD16 Monoclonal antibody PE Conjugated, Flow Validated as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-06
Question
I have attached the WB image, lot number and protocol we used for lung using anti-human CD16 Monoclonal antibody PE Conjugated, Flow Validated FC01408-PE. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-17
Question
I see that the anti-human CD16 Monoclonal antibody PE Conjugated, Flow Validated FC01408-PE works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-09-16
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-09-16
Question
My question regards to test anti-human CD16 Monoclonal antibody PE Conjugated, Flow Validated FC01408-PE on human lung for research purposes, then I may be interested in using anti-human CD16 Monoclonal antibody PE Conjugated, Flow Validated FC01408-PE for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
The products we sell, including anti-human CD16 Monoclonal antibody PE Conjugated, Flow Validated FC01408-PE, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-20
Question
Is a blocking peptide available for product anti-human CD16 Monoclonal antibody PE Conjugated, Flow Validated (FC01408-PE)?
G. Krishna
Verified customer
Asked: 2019-03-22
Answer
We do provide the blocking peptide for product anti-human CD16 Monoclonal antibody PE Conjugated, Flow Validated (FC01408-PE). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-03-22
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-human CD16 Monoclonal antibody PE Conjugated, Flow Validated FC01408-PE. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-01-18
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-18
Question
Will anti-human CD16 Monoclonal antibody PE Conjugated, Flow Validated FC01408-PE work for Flow Cytometry with lung?
Verified Customer
Verified customer
Asked: 2018-08-22
Answer
According to the expression profile of lung, FCGR3A is highly expressed in lung. So, it is likely that anti-human CD16 Monoclonal antibody PE Conjugated, Flow Validated FC01408-PE will work for Flow Cytometry with lung.
Boster Scientific Support
Answered: 2018-08-22
Question
I have a question about product FC01408-PE, anti-human CD16 Monoclonal antibody PE Conjugated, Flow Validated. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-11-10
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free FC01408-PE anti-human CD16 Monoclonal antibody PE Conjugated, Flow Validated, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-11-10
Question
I was wanting to use your anti-human CD16 Monoclonal antibody PE Conjugated, Flow Validated for Flow Cytometry for human lung on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human lung identification?
S. Thomas
Verified customer
Asked: 2017-08-30
Answer
As indicated on the product datasheet, FC01408-PE anti-human CD16 Monoclonal antibody PE Conjugated, Flow Validated has been tested for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-30
Question
Is this FC01408-PE anti-human CD16 Monoclonal antibody PE Conjugated, Flow Validated reactive to the isotypes of FCGR3A?
G. Taylor
Verified customer
Asked: 2014-06-13
Answer
The immunogen of FC01408-PE anti-human CD16 Monoclonal antibody PE Conjugated, Flow Validated is Human peripheral blood T lymphocytes. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2014-06-13
