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Product Info Summary
| SKU: | A01408-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IF, IHC, ICC |
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Product info
Product Name
Anti-CD16/FCGR3A Antibody
SKU/Catalog Number
A01408-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD16/FCGR3A Antibody catalog # A01408-1. Tested in Direct ELISA, FCM, ICC/IF, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD16/FCGR3A Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01408-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human CD16 recombinant protein (Position: Q101-D166).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A01408-1 is reactive to FCGR3A in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
45 kDa
Calculated molecular weight
29.089kDa
Background of FCGR3A
Low affinity immunoglobulin gamma Fc region receptor III-A is a protein that in humans is encoded by the FCGR3A gene. This gene encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. This gene (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by this gene is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in this gene have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01408-1 is guaranteed for ELISA, Flow Cytometry, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml
Flow Cytometry (Fixed), 1-3μg/1x106 cells
ELISA, 0.1-0.5μg/ml
Positive Control
IHC: human liver cancer tissue, human rectal cancer tissue, human rectal cancer tissue
ICC/IF: K562 cell
FCM: HL-60 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. IHC analysis of CD16 using anti-CD16 antibody (A01408-1).
CD16 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD16 Antibody (A01408-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 2. IHC analysis of CD16 using anti-CD16 antibody (A01408-1).
CD16 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD16 Antibody (A01408-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of CD16 using anti-CD16 antibody (A01408-1).
CD16 was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-CD16 Antibody (A01408-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 4. IF analysis of CD16 using anti-CD16 antibody (A01408-1).
CD16 was detected in an immunocytochemical section of K562 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-CD16 Antibody (A01408-1) overnight at 4°C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Click image to see more details
Figure 5. Flow Cytometry analysis of human PBMC cells using anti-CD16 antibody (A01408-1).
Overlay histogram showing human PBMC cells stained with A01408-1 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-CD16 Antibody (A01408-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For FCGR3A (Source: Uniprot.org, NCBI)
Gene Name
FCGR3A
Full Name
Low affinity immunoglobulin gamma Fc region receptor III-A
Weight
29.089kDa
Alternative Names
Low affinity immunoglobulin gamma Fc region receptor III-A; CD16a antigen; Fc-gamma RIII-alpha; Fc-gamma RIII; Fc-gamma RIIIa; FcRIII; FcRIIIa; FcR-10; IgG Fc receptor III-2; CD16a; FCGR3A; CD16A; FCG3; FCGR3; IGFR3 FCGR3A CD16, CD16A, FCG3, FCGR3, FCGRIII, FCR-10, FCRIII, FCRIIIA, IGFR3, IMD20 Fc fragment of IgG receptor IIIa low affinity immunoglobulin gamma Fc region receptor III-A|CD16a |Fc fragment of IgG, low affinity III, receptor for (CD16)|Fc fragment of IgG, low affinity IIIa, receptor (CD16a)|Fc gamma receptor III-A|Fc-gamma RIII-alpha|Fc-gamma receptor III-2 (CD 16)|Fc-gamma receptor IIIb (CD16)|FcgammaRIIIA|igG Fc receptor III-2|immunoglobulin G Fc receptor III|low affinity immunoglobulin gamma receptor III-a Fc fragment|neutrophil-specific NA
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FCGR3A, check out the FCGR3A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FCGR3A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD16/FCGR3A Antibody (A01408-1)
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Customer Q&As
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17 Customer Q&As for Anti-CD16/FCGR3A Antibody
Question
I have a question about product A01408-1, anti-CD16/FCGR3A antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A01408-1 anti-CD16/FCGR3A antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-24
Question
We are currently using anti-CD16/FCGR3A antibody A01408-1 for rat tissue, and we are satisfied with the ELISA results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
The anti-CD16/FCGR3A antibody (A01408-1) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-16
Question
I see that the anti-CD16/FCGR3A antibody A01408-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-26
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-26
Question
Our lab used your anti-CD16/FCGR3A antibody for ELISA on leukocyte in a previous project. I am using human, and I plan to use the antibody for WB next. Our lab want to know about examining leukocyte as well as lung in our next experiment. Could give a recommendation on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-07-23
Answer
I took a look at the website and datasheets of our anti-CD16/FCGR3A antibody and I see that A01408-1 has been validated on human in both ELISA and WB. Thus A01408-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-23
Question
I was wanting to use your anti-CD16/FCGR3A antibody for WB for human lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lung identification?
Verified Customer
Verified customer
Asked: 2019-07-12
Answer
You can see on the product datasheet, A01408-1 anti-CD16/FCGR3A antibody has been tested for ELISA, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-07-12
Question
Is a blocking peptide available for product anti-CD16/FCGR3A antibody (A01408-1)?
T. Banerjee
Verified customer
Asked: 2019-07-03
Answer
We do provide the blocking peptide for product anti-CD16/FCGR3A antibody (A01408-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-03
Question
Does A01408-1 react to CD32 and can it be used for IF staining with mouse cells?
Verified customer
Asked: 2019-04-11
Answer
We tested the Anti-CD16/FCGR3A Antibody Picoband™ (A01408-1) cross reactivity with CD32 and no obvious cross reactivity was observed. We did not observe any positive IF staining on mouse cell sample.
Boster Scientific Support
Answered: 2019-04-22
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-CD16/FCGR3A antibody A01408-1. Let me know if you need anything else.
E. Johnson
Verified customer
Asked: 2017-11-17
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-17
Question
We have seen staining in mouse blood. What should we do? Is anti-CD16/FCGR3A antibody supposed to stain blood positively?
Verified Customer
Verified customer
Asked: 2017-11-14
Answer
According to literature blood does express FCGR3A. According to Uniprot.org, FCGR3A is expressed in leukocyte, blood, synovium, lung, placenta, among other tissues. Regarding which tissues have FCGR3A expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 16951347
Lung, Pubmed ID: 2525780, 15489334
Placenta, Pubmed ID: 7836402
Synovium, Pubmed ID: 16710414
Boster Scientific Support
Answered: 2017-11-14
Question
We want using your anti-CD16/FCGR3A antibody for role of phospholipids in phagocytosis studies. Has this antibody been tested with western blotting on mouse nih3t3 whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-10-11
Answer
We appreciate your inquiry. This A01408-1 anti-CD16/FCGR3A antibody is tested on mouse nih3t3 whole cell lysates. It is guaranteed to work for ELISA, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-10-11
Question
Is there a BSA free version of anti-CD16/FCGR3A antibody A01408-1 available?
Verified Customer
Verified customer
Asked: 2017-07-17
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-CD16/FCGR3A antibody A01408-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2017-07-17
Question
Would A01408-1 anti-CD16/FCGR3A antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-06-09
Answer
It shows on the product datasheet, A01408-1 anti-CD16/FCGR3A antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-09
Question
Is this A01408-1 anti-CD16/FCGR3A antibody reactive to the isotypes of FCGR3A?
V. Gonzalez
Verified customer
Asked: 2015-02-06
Answer
The immunogen of A01408-1 anti-CD16/FCGR3A antibody is E. coli-derived human CD16 recombinant protein (Position: Q101-D166). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-02-06
Question
We are interested in to test anti-CD16/FCGR3A antibody A01408-1 on human lung for research purposes, then I may be interested in using anti-CD16/FCGR3A antibody A01408-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
G. Kulkarni
Verified customer
Asked: 2013-06-21
Answer
The products we sell, including anti-CD16/FCGR3A antibody A01408-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2013-06-21
Question
Will anti-CD16/FCGR3A antibody A01408-1 work for WB with lung?
R. Walker
Verified customer
Asked: 2013-02-14
Answer
According to the expression profile of lung, FCGR3A is highly expressed in lung. So, it is likely that anti-CD16/FCGR3A antibody A01408-1 will work for WB with lung.
Boster Scientific Support
Answered: 2013-02-14
Question
My team were content with the WB result of your anti-CD16/FCGR3A antibody. However we have observed positive staining in lung cell membrane using this antibody. Is that expected? Could you tell me where is FCGR3A supposed to be expressed?
F. Yang
Verified customer
Asked: 2013-01-30
Answer
Based on literature, lung does express FCGR3A. Generally FCGR3A expresses in cell membrane. Regarding which tissues have FCGR3A expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 16951347
Lung, Pubmed ID: 2525780, 15489334
Placenta, Pubmed ID: 7836402
Synovium, Pubmed ID: 16710414
Boster Scientific Support
Answered: 2013-01-30
Question
Please see the WB image, lot number and protocol we used for lung using anti-CD16/FCGR3A antibody A01408-1. Please let me know if you require anything else.
M. Jha
Verified customer
Asked: 2013-01-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-01-03
