Product Info Summary
SKU: | RP1103 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human |
Host: | Rabbit |
Application: | Flow Cytometry, IHC |
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Product info
Product Name
Anti-CD153/TNFSF8 Antibody
View all CD30 Ligand/TNFSF8 Antibodies
SKU/Catalog Number
RP1103
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-CD153/TNFSF8 Antibody catalog # RP1103. Tested in Flow Cytometry, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-CD153/TNFSF8 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # RP1103)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human CD153 recombinant protein (Position: Q63-D234). Human CD153 shares 75% amino acid (aa) sequence identity with mouse CD153.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
RP1103 is reactive to TNFSF8 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
86 kDa
Calculated molecular weight
26017 MW
Background of CD30 Ligand/TNFSF8
CD153 is a ligand for CD30. The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF8/CD30, which is a cell surface antigen and a marker for Hodgkin lymphoma and related hematologic malignancies. The engagement of this cytokine expressed on B cell surface plays an inhibitory role in modulating Ig class switch. This cytokine was shown to enhance cell proliferation of some lymphoma cell lines, while to induce cell death and reduce cell proliferation of other lymphoma cell lines. The pleiotropic biologic activities of this cytokine on different CD30+ lymphoma cell lines may play a pathophysiologic role in Hodgkin's and some non-Hodgkin's lymphomas. Two transcript variants encoding different isoforms have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
RP1103 is guaranteed for Flow Cytometry, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, By Heat
Flow Cytometry, 1-3μg/1x106cells
Positive Control
IHC: human tonsil tissue
FCM: U937 cell
Validation Images & Assay Conditions
Click image to see more details
CD153 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti-CD153 Antigen Affinity purified polyclonal antibody (Catalog # RP1103) at 1 μg/mL. The immunohistochemical section was developed using SABC method (Catalog # SA1022).
Click image to see more details
2. Flow Cytometry analysis of U937 cells using anti-CD153 antibody (RP1103).
Overlay histogram showing U937 cells stained with RP1103 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD153 Antibody (RP1103,1μg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For TNFSF8 (Source: Uniprot.org, NCBI)
Gene Name
TNFSF8
Full Name
Tumor necrosis factor ligand superfamily member 8
Weight
26017 MW
Superfamily
tumor necrosis factor family
Alternative Names
Tumor necrosis factor ligand superfamily member 8;CD30 ligand;CD30-L;CD153;TNFSF8;CD30L, CD30LG; TNFSF8 CD153, CD30L, CD30LG, TNLG3A TNF superfamily member 8 tumor necrosis factor ligand superfamily member 8|CD153 |CD30 ligand|CD30 ligand|CD30-L|tumor necrosis factor (ligand) superfamily, member 8|tumor necrosis factor ligand 3A|tumor necrosis factor superfamily member 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TNFSF8, check out the TNFSF8 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TNFSF8: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-CD153/TNFSF8 Antibody (RP1103)
Hello CJ!
RP1103 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
CD30 Is Highly Expressed in Chronic Obstructive Pulmonary Disease and Induces the Pulmonary Vascular Remodeling
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1 Customer Q&As for Anti-CD153/TNFSF8 Antibody
Question
We are currently using anti-CD153/TNFSF8 antibody RP1103 for human tissue, and we are content with the IHC results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
The anti-CD153/TNFSF8 antibody (RP1103) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-04-06