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Product Info Summary
| SKU: | EK0569 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human CD30/TNFRSF8 ELISA Kit PicoKine®
View all CD30/TNFRSF8 ELISA kits
SKU/Catalog Number
EK0569
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human CD30/TNFRSF8 ELISA Kit PicoKine® (96 Tests). Quantitate Human TNFRSF8 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human CD30/TNFRSF8 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0569)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: F19-K379
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0569 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0569 is reactive to TNFRSF8 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0569 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CD30/TNFRSF8
CD30, also known as TNFRSF8, is a cell membrane protein of the tumor necrosis factor receptor family and tumor marker. This receptor is a positive regulator of apoptosis, and also has been shown to limit the proliferative potential of autoreactive CD8 effector T cells and protect the body against autoimmunity. This gene is mapped to 1p36.22. CD30 is expressed in embryonal carcinoma but not in seminoma and is thus a useful marker in distinguishing between these germ cell tumors. CD30 mast cell activation represents an IgE-independent activation pathway, which is important for understanding cutaneous inflammation associated with mast cells. In addition to those, CD30 is also associated with anaplastic large cell lymphoma.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0569-CAP | Anti-Human TNFRSF8 Pre-coated 96-well strip microplate | 1 |
| EK0569-ST | Human TNFRSF8 Standard | 2 vials, 10 ng/tube |
| EK0569-DA | Human TNFRSF8 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.039 | 0.173 | 0.281 | 0.472 | 0.784 | 1.236 | 1.732 | 2.010 |
Data Example Images
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Human CD30/TNFRSF8 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human CD30/TNFRSF8 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 321 | 1322 | 3947 | 305 | 1371 | 3830 |
| Standard deviation | 25.03 | 64.77 | 157.88 | 28.06 | 93.22 | 214.48 |
| CV (%) | 7.8% | 4.9% | 4% | 9.2% | 6.8% | 5.6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of CD30/TNFRSF8 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 321 | 324 | 313 | 319 | 319 | 4.02 | 1.2% |
| Sample 2 | 1322 | 1420 | 1463 | 1452 | 1414 | 55.55 | 3.9% |
| Sample 3 | 3947 | 3780 | 4218 | 4450 | 4098 | 256.04 | 6.2% |
Protein Target Info & Infographic
Gene/Protein Information For TNFRSF8 (Source: Uniprot.Org, NCBI)
Gene Name
TNFRSF8
Full Name
Tumor necrosis factor receptor superfamily member 8
Weight
63747 MW
Superfamily
TNFR8 family
Alternative Names
Tumor necrosis factor receptor superfamily member 8;CD30L receptor;Ki-1 antigen;Lymphocyte activation antigen CD30;CD30;TNFRSF8;CD30, D1S166E; TNFRSF8 CD30, D1S166E, Ki-1 TNF receptor superfamily member 8 tumor necrosis factor receptor superfamily member 8|CD30L receptor|Ki-1 |cytokine receptor CD30|lymphocyte activation CD30
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on TNFRSF8, check out the TNFRSF8 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFRSF8: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Human CD30/TNFRSF8 ELISA Kit PicoKine® (EK0569)
Hello CJ!
EK0569 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Specific ADAM10 inhibitors localize in exosome-like vesicles released by Hodgkin lymphoma and stromal cells and prevent sheddase activity carried to bystander cells
Memory stem T cells modified with a redesigned CD30‐chimeric antigen receptor show an enhanced antitumor effect in Hodgkin lymphoma
Tosetti F1, Ven
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Customer Reviews
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Customer Q&As
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9 Customer Q&As for Human CD30/TNFRSF8 ELISA Kit PicoKine®
Question
Does EK0569 contain native human biological materials?
Verified customer
Asked: 2021-11-10
Answer
The Human CD30/TNFRSF8 ELISA Kit PicoKine® (EK0569) doesn't contain native human biological materials.
Boster Scientific Support
Answered: 2021-11-11
Question
Q: is it okay to use citrate plasma as samples in Human CD30 Picokine® ELISA Kit (Catalog # EK0569)?
H. Moore
Verified customer
Asked: 2020-06-04
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of CD30 causing disruption of its protein structure. CD30 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the CD30 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-06-04
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
E. Yang
Verified customer
Asked: 2019-08-14
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-08-14
Question
Q: if the enzyme conjugated CD30 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD30 antigen?
Verified Customer
Verified customer
Asked: 2018-12-30
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-12-30
Question
Q: how to thaw whole blood sample for CD30 ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-09-04
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test CD30 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2018-09-04
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-07-17
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-07-17
Question
Q: how to proceed with the analysis of ELISA data? I measured CD30 level in plasma.
Verified Customer
Verified customer
Asked: 2016-05-18
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-05-18
Question
Q: can you recommend the dilution ratio of serum samples for detection of CD30 in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2015-11-29
Answer
A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the CD30 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2015-11-29
Question
Hello Boster: I would like to ask the concentration of EK0569 for isotype matching.
Verified Customer
Verified customer
Asked: 2013-11-07
Answer
The antibody EK0569 is sold as tissue culture supernatant. The unpurified antibodies, those sold as whole antiserum, ascites or tissue culture supernatant does not have a concentration stated on the datasheet. However, antibody concentration is usually determined by protein assay and tissue culture supernatant. Yet, we suggest that the concentration of antibody is known to very between 1 - 3 mg/ml.
Boster Scientific Support
Answered: 2013-11-07
