Click image to see more details
-
-
-
-
-
+3
Product Info Summary
| SKU: | PB9082 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-GFAP Antibody Picoband®
SKU/Catalog Number
PB9082
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-GFAP Antibody Picoband® catalog # PB9082. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat, Pig. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-GFAP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9082)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human GFAP recombinant protein (Position: Q93-M432). Human GFAP shares 94% amino acid (aa) sequence identity with both mouse and rat GFAP.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9082 is reactive to GFAP in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
50 kDa
Calculated molecular weight
49880 MW
Background of GFAP
Glial fibrillary acidic protein (GFAP) is a protein that is encoded by the GFAP gene in humans. It is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes, and ependymal cells. It is mapped to 17q21.31. GFAP is closely related to its non-epithelial family members, vimentin, desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength, as well as the shape of cells. This gene has been shown to play a role in mitosis by adjusting the filament network present in the cell. GFAP is necessary for many critical roles in the CNS. What’s more, GFAP also plays a role in astrocyte-neuron interactions as well as cell-cell communication.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9082 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, Pig, By Heat
Immunofluorescence, 5μg/ml, Rat
Positive Control
WB: human U251 whole cell, human Hela whole cell, rat brain tissue, rat C5 whole cell, mouse brain tissue
IHC: human glioma tissue, mouse brain tissue, mouse brain tissue, rat brain tissue, pig brain tissue
IF: rat brain tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of GFAP using anti-GFAP antibody (PB9082).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human U251 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat C5 whole cell lysates,
Lane 5: mouse brain tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GFAP antigen affinity purified polyclonal antibody (Catalog # PB9082) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for GFAP at approximately 50 kDa. The expected band size for GFAP is at 50 kDa.
Click image to see more details
Figure 2. IHC analysis of GFAP using anti-GFAP antibody (PB9082).
GFAP was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFAP Antibody (PB9082) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of GFAP using anti-GFAP antibody (PB9082).
GFAP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFAP Antibody (PB9082) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 4. IHC analysis of GFAP using anti-GFAP antibody (PB9082).
GFAP was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFAP Antibody (PB9082) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 5. IHC analysis of GFAP using anti-GFAP antibody (PB9082).
GFAP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFAP Antibody (PB9082) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 6. IHC analysis of GFAP using anti-GFAP antibody (PB9082).
GFAP was detected in a paraffin-embedded section of pig brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-GFAP Antibody (PB9082) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 7. IF analysis of GFAP using anti-GFAP antibody (PB9082).
GFAP was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 μg/mL rabbit anti-GFAP Antibody (PB9082) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For GFAP (Source: Uniprot.org, NCBI)
Gene Name
GFAP
Full Name
Glial fibrillary acidic protein
Weight
49880 MW
Superfamily
intermediate filament family
Alternative Names
Glial fibrillary acidic protein;GFAP;GFAP; GFAP ALXDRD glial fibrillary acidic protein glial fibrillary acidic protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on GFAP, check out the GFAP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for GFAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-GFAP Antibody Picoband® (PB9082)
Hello CJ!
PB9082 has been cited in 137 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Glucagon-like peptide-1/glucose-dependent insulinotropic polypeptide dual receptor agonist DA-CH5 is superior to exendin-4 in protecting neurons in the 6-hydroxydopamine rat Parkinson model
Effect of electroacupuncture on glial fibrillary acidic protein and nerve growth factor in the hippocampus of rats with hyperlipidemia and middle cerebral artery thrombus
Effect of all-trans retinoic acid on the proliferation and differentiation of brain tumor stem cells
Electroacupuncture alleviates spatial memory deficits in METH withdrawal mice by enhancing astrocyte-mediated glutamate clearance in the dCA1
Klotho ameliorated cognitive deficits in a temporal lobe epilepsy rat model by inhibiting ferroptosis
Differentiation of Mesenchymal Stem Cells into Neural Stem Cells Using Cerebrospinal Fluid
Astrocyte-selective STAT3 knockdown rescues methamphetamine withdrawal-disrupted spatial memory in mice via restoring the astrocytic capacity of glutamate clearance in dCA1
Tak1 in the astrocytes of mediobasal hypothalamus regulates anxiety‐like behavior in mice
Maternal Benzophenone Exposure Impairs Hippocampus Development and Cognitive Function in Mouse Offspring
Maternal Benzophenone Exposure Impairs Hippocampus Development and Cognitive Function in Mouse Offspring
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-GFAP Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
1 Reviews For Anti-GFAP Antibody Picoband®
0
The antibody worked well even though we used fresh frozen brain sections cut at 20 micron thickness thaw-mounted onto microscope slide that were then post-fixed with 4% paraformaldehyde. The antibody worked equally well when used on fixed brains sectioned
Excellent
Boster bio GFAP 1:100 PFA (7-2) - cortex 12x - with scale bar
Boster bio GFAP 1:100 PFA (7-2) - cortex 20x - with scale bar
Boster bio GFAP 1:250 12x (free-floating)
| Application | Immunofluorescence |
|---|---|
| Sample | Mouse brain |
| Primary Antibody Dilution | 1:100 |
Images that were made from fresh frozen cryostat sections, were thaw-mounted onto microscope slides. Mounted sections were later post-fixed with 300 µL of 4% paraformaldehyde (PFA) in PBS at room temperature for 10 minutes. After fixation and prior to incubation with antibodies, slides were washed 3 times with 300 µL of 1X PBS with 0.01% sodium azide for 3 minutes per rinse, followed by permeabilization with 300 µL of 0.3% Triton X-100 in 1X PBS with 0.01% sodium azide for 30 minutes at room temperature, then blocking with 700 µL of 4% donkey serum diluted in 1X PBS with 0.01% sodium azide + Triton X-100 (blocking buffer) for 30 minutes at room temperature. Slides were incubated with 325 µL of GFAP (1:100). Concentrations of 1:100 were achieved by diluting 10 µL of antibody in 1,000 µL of blocking buffer; concentrations of 1:250 were achieved by diluting 4 µL of antibody in 1,000 µL of blocking buffer. Sections were incubated overnight at 4 ˚C. On the next day, slides were washed 3 times with 300 µL of 1X PBS with 0.01% sodium azide for 3 minutes per rinse, then incubated with the secondary antibodies at room temperature, in the dark, for 1 hour. Washing step was repeated then slides were left in the dark to dry. Mounting media was added to cover slip the sections. Slides were kept in the dark at 4 ˚C prior to imaging.
Immunohistochemistry was also performed with free-floating sections, which were exposed to the same primary antibodies diluted (1:250) in blocking buffer overnight and, subsequently, exposed to secondary antibodies diluted in blocking buffer for 1 hour, following the same procedure as the mounted sections. After incubation, sections were washed 3 times with 300 µL of 1X PBS with 0.01% sodium azide for 3 minutes per rinse and transferred with a painting brush to a container filled with 1X PBS with 0.01% sodium azide, from which they were mounted onto slides. Slides were left to dry in the dark, after which mounting media was added to cover slip the sections. As with slide-mounted sections, these slides were kept in the dark at 4 ˚C prior to imaging.
Bob Speth from Nova Southeastern University
Verified customer
Submitted 2024-06-19
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
16 Customer Q&As for Anti-GFAP Antibody Picoband®
Question
I see that the anti-GFAP antibody PB9082 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-23
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-23
Question
I have attached the WB image, lot number and protocol we used for fetal brain using anti-GFAP antibody PB9082. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-02-28
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-02-28
Question
Is this PB9082 anti-GFAP antibody reactive to the isotypes of GFAP?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
The immunogen of PB9082 anti-GFAP antibody is E.coli-derived human GFAP recombinant protein (Position: Q93-M432). Human GFAP shares 94% amino acid (aa) sequence identity with both mouse and rat GFAP. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-02-04
Question
My team were well pleased with the WB result of your anti-GFAP antibody. However we have observed positive staining in kidney cytoplasm. using this antibody. Is that expected? Could you tell me where is GFAP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-01-06
Answer
Based on literature, kidney does express GFAP. Generally GFAP expresses in cytoplasm. Regarding which tissues have GFAP expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 12837269
Brain, Pubmed ID: 15489334
Brain, and Thalamus, Pubmed ID: 14702039
Fetal brain, Pubmed ID: 12058025
Fetal brain cortex, Pubmed ID: 2780570
Kidney, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2020-01-06
Question
We are currently using anti-GFAP antibody PB9082 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2019-12-18
Answer
The anti-GFAP antibody (PB9082) has not been tested for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-18
Question
We have seen staining in mouse fetal brain. What should we do? Is anti-GFAP antibody supposed to stain fetal brain positively?
Verified Customer
Verified customer
Asked: 2019-10-29
Answer
From literature fetal brain does express GFAP. From Uniprot.org, GFAP is expressed in dorsal motor nucleus of vagus nerve, brain thalamus, brain, kidney, fetal brain cortex, fetal brain, blood, among other tissues. Regarding which tissues have GFAP expression, here are a few articles citing expression in various tissues:
Blood, Pubmed ID: 12837269
Brain, Pubmed ID: 15489334
Brain, and Thalamus, Pubmed ID: 14702039
Fetal brain, Pubmed ID: 12058025
Fetal brain cortex, Pubmed ID: 2780570
Kidney, Pubmed ID: 17974005
Boster Scientific Support
Answered: 2019-10-29
Question
Do you have a BSA free version of anti-GFAP antibody PB9082 available?
Verified Customer
Verified customer
Asked: 2019-09-19
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-GFAP antibody PB9082 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-09-19
Question
Will PB9082 anti-GFAP antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-13
Answer
As indicated on the product datasheet, PB9082 anti-GFAP antibody as been tested on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-13
Question
Will anti-GFAP antibody PB9082 work for IHC with fetal brain?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
According to the expression profile of fetal brain, GFAP is highly expressed in fetal brain. So, it is likely that anti-GFAP antibody PB9082 will work for IHC with fetal brain.
Boster Scientific Support
Answered: 2019-08-08
Question
Is a blocking peptide available for product anti-GFAP antibody (PB9082)?
Verified Customer
Verified customer
Asked: 2019-07-15
Answer
We do provide the blocking peptide for product anti-GFAP antibody (PB9082). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-15
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for fetal brain using anti-GFAP antibody PB9082. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-06
Question
My question regarding product PB9082, anti-GFAP antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-05-23
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9082 anti-GFAP antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-05-23
Question
I was wanting to use your anti-GFAP antibody for IHC for rat fetal brain on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat fetal brain identification?
Verified Customer
Verified customer
Asked: 2019-04-18
Answer
As indicated on the product datasheet, PB9082 anti-GFAP antibody has been tested for IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat fetal brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-04-18
Question
We ordered your anti-GFAP antibody for WB on dorsal motor nucleus of vagus nerve a few months ago. I am using rat, and I plan to use the antibody for IHC next. I was wanting to use examining dorsal motor nucleus of vagus nerve as well as kidney in our next experiment. Could give a recommendation on which antibody would work the best for IHC?
Verified Customer
Verified customer
Asked: 2018-07-26
Answer
I looked at the website and datasheets of our anti-GFAP antibody and I see that PB9082 has been tested on rat in both WB and IHC. Thus PB9082 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-07-26
Question
My question regards to test anti-GFAP antibody PB9082 on rat fetal brain for research purposes, then I may be interested in using anti-GFAP antibody PB9082 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
S. Mitchell
Verified customer
Asked: 2017-04-19
Answer
The products we sell, including anti-GFAP antibody PB9082, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-04-19
Question
We need using your anti-GFAP antibody for chaperone mediated autophagy studies. Has this antibody been tested with western blotting on neuro whole cell lysate? We would like to see some validation images before ordering.
K. Brown
Verified customer
Asked: 2013-05-10
Answer
Thank you for your inquiry. This PB9082 anti-GFAP antibody is tested on mouse brain, brain tissue, tissue lysate, rat brain tissue, human meningioma tissue, u87 whole cell lysate, shg whole cell lysate, neuro whole cell lysate, hela whole cell lysate. It is guaranteed to work for IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2013-05-10
