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Product Info Summary
| SKU: | PB9332 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-Caspase-9/CASP9 Antibody Picoband®
SKU/Catalog Number
PB9332
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caspase-9/CASP9 Antibody Picoband® catalog # PB9332. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase-9/CASP9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9332)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Caspase-9 recombinant protein (Position: E3-D228). Human Caspase-9 shares 63% amino acid (aa) sequence identity with mouse Caspase-9.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9332 is reactive to CASP9 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
35 kDa
Calculated molecular weight
46281 MW
Background of Caspase-9
CASP9 is also known as MCH6 or APAF3. This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein can undergo autoproteolytic processing and activation by the apoptosome, a protein complex of cytochrome c and the apoptotic peptidase activating factor 1; this step is thought to be one of the earliest in the caspase activation cascade. This protein is thought to play a central role in apoptosis and to be a tumor suppressor. Alternative splicing results in multiple transcript variants.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9332 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: A549 Whole Cell, SMMC Whole Cell, 293T Whole Cell, JURKAT Whole Cell, RAJI Whole Cell, CEM Whole Cell, HUT Whole Cell
Validation Images & Assay Conditions
Click image to see more details
Anti-Caspase-9 Picoband antibody, PB9332, Western blotting
All lanes: Anti Caspase-9 (PB9332) at 0.5ug/ml
Lane 1: A549 Whole Cell Lysate at 40ug
Lane 2: SMMC Whole Cell Lysate at 40ug
Lane 3: 293T Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: RAJI Whole Cell Lysate at 40ug
Lane 6: CEM Whole Cell Lysate at 40ug
Lane 7: HUT Whole Cell Lysate at 40ug
Predicted bind size: 35KD
Observed bind size: 35KD
Protein Target Info & Infographic
Gene/Protein Information For CASP9 (Source: Uniprot.org, NCBI)
Gene Name
CASP9
Full Name
Caspase-9
Weight
46281 MW
Superfamily
peptidase C14A family
Alternative Names
Caspase-9;CASP-9;3.4.22.62;Apoptotic protease Mch-6;Apoptotic protease-activating factor 3;APAF-3;ICE-like apoptotic protease 6;ICE-LAP6;Caspase-9 subunit p35;Caspase-9 subunit p10;CASP9;MCH6; CASP9 APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56 caspase 9 caspase-9|ICE-like apoptotic protease 6|apoptotic protease MCH-6|apoptotic protease activating factor 3|caspase 9, apoptosis-related cysteine peptidase|protein phosphatase 1, regulatory subunit 56
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP9, check out the CASP9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase-9/CASP9 Antibody Picoband® (PB9332)
Hello CJ!
PB9332 has been cited in 17 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
In Vitro Effects of HAS-2 Gene Silencing on the Proliferation and Apoptosis of the MCF-7 Human Breast Cancer Cell Line
Chinese herbal medicine Yougui Pill reduces exogenous glucocorticoid-induced apoptosis in anterior pituitary cells
In vivo?and?in vitro?evaluation of the cytotoxic effects of Photosan-loaded hollow silica nanoparticles on liver cancer
Chang C, Liu Sp, Fang Ch, He Rs, Wang Z, Zhu Yq, Jiang Sw. Oncol Lett. 2013 Sep;6(3):699-704. Epub 2013 Jul 8. Effects Of Matrine On The Proliferation Of Ht29 Human Colon Cancer Cells And Its Antitumor Mechanism.
Yuan X, Sun Q, Ou Y, Wang S, Zhang W, Deng H, Wu X, Zhang L. Neural Regen Res. 2014 Apr 15;9(8):837-44. Doi: 10.4103/1673-5374.131600. Apoptosis Is An Obstacle To The Differentiation Of Adipose-Derived Stromal Cells Into Astrocytes.
Chen S, Peng H, Rowat A, Gao F, Zhang Z, Wang P, Zhang W, Wang X, Qu L. Brain Res. 2015 Aug 27;1618:205-11. Doi: 10.1016/J.Brainres.2015.05.027. Epub 2015 May 30. The Effect Of Concentration And Duration Of Normobaric Oxygen In Reducing Caspase-3 ...
Dai C, Li J, Tang S, Li J, Xiao X. Antimicrob Agents Chemother. 2014 Jul;58(7):4075-85. Doi: 10.1128/Aac.00070-14. Epub 2014 May 5. Colistin-Induced Nephrotoxicity In Mice Involves The Mitochondrial, Death Receptor, And Endoplasmic Reticulum Pathw...
Huang C, Lin Y, Su H, Ye D. Neurochem Res. 2015 Jan;40(1):27-35. Doi: 10.1007/S11064-014-1461-5. Epub 2014 Oct 25. Forsythiaside Protects Against Hydrogen Peroxide-Induced Oxidative Stress And Apoptosis In Pc12 Cell.
Yang Y, Li Zh, Liu H, Shi Wd, Zhang J. Chin J Integr Med. 2015 Jun;21(6):423-30. Doi: 10.1007/S11655-014-1752-3. Epub 2014 May 10. Inhibitory Effect Of Tetramethylpyrazine Preconditioning On Overload Training-Induced Myocardial Apoptosis In Rats.
Song Gh, Wang Rl, Chen Zy, Zhang B, Wang Hl, Liu Ml, Gao Jp, Yan Xy. J Physiol Biochem. 2014 Sep;70(3):761-8. Doi: 10.1007/S13105-014-0344-1. Epub 2014 Jul 30. Toxic Effects Of Sodium Fluoride On Cell Proliferation And Apoptosis Of Leydig Cells Fr...
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Customer Reviews
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Customer Q&As
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15 Customer Q&As for Anti-Caspase-9/CASP9 Antibody Picoband®
Question
I was wanting to use your anti-Caspase-9/CASP9 antibody for WB for human adrenal tissue on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human adrenal tissue identification?
Verified Customer
Verified customer
Asked: 2020-04-30
Answer
You can see on the product datasheet, PB9332 anti-Caspase-9/CASP9 antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human adrenal tissue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-30
Question
I am interested in to test anti-Caspase-9/CASP9 antibody PB9332 on human adrenal tissue for research purposes, then I may be interested in using anti-Caspase-9/CASP9 antibody PB9332 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
The products we sell, including anti-Caspase-9/CASP9 antibody PB9332, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-10
Question
Please see the WB image, lot number and protocol we used for adrenal tissue using anti-Caspase-9/CASP9 antibody PB9332. Please let me know if you require anything else.
L. Gonzalez
Verified customer
Asked: 2020-01-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-01-22
Question
Does anti-Caspase-9/CASP9 antibody PB9332 work for WB with adrenal tissue?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
According to the expression profile of adrenal tissue, CASP9 is highly expressed in adrenal tissue. So, it is likely that anti-Caspase-9/CASP9 antibody PB9332 will work for WB with adrenal tissue.
Boster Scientific Support
Answered: 2020-01-15
Question
I have a question about product PB9332, anti-Caspase-9/CASP9 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-11-01
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9332 anti-Caspase-9/CASP9 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-11-01
Question
Is a blocking peptide available for product anti-Caspase-9/CASP9 antibody (PB9332)?
H. Jha
Verified customer
Asked: 2019-10-15
Answer
We do provide the blocking peptide for product anti-Caspase-9/CASP9 antibody (PB9332). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-10-15
Question
We want using your anti-Caspase-9/CASP9 antibody for akt phosphorylates targets in the cytosol studies. Has this antibody been tested with western blotting on hut whole cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-09-11
Answer
We appreciate your inquiry. This PB9332 anti-Caspase-9/CASP9 antibody is validated on a549 whole cell lysate, smmc whole cell lysate, 293t whole cell lysate, jurkat whole cell lysate, raji whole cell lysate, cem whole cell lysate, hut whole cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-09-11
Question
We have been able to see staining in human kidney. Do you have any suggestions? Is anti-Caspase-9/CASP9 antibody supposed to stain kidney positively?
Verified Customer
Verified customer
Asked: 2019-08-30
Answer
According to literature kidney does express CASP9. According to Uniprot.org, CASP9 is expressed in adrenal tissue, t-cell, stomach cancer, kidney, eye lymph, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CASP9 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Eye, and Lymph, Pubmed ID: 15489334
Kidney, Pubmed ID: 14702039
Stomach cancer, Pubmed ID: 9890966
T-cell, Pubmed ID: 8900201
Boster Scientific Support
Answered: 2019-08-30
Question
I see that the anti-Caspase-9/CASP9 antibody PB9332 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-29
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adrenal tissue using anti-Caspase-9/CASP9 antibody PB9332. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-27
Question
Is this PB9332 anti-Caspase-9/CASP9 antibody reactive to the isotypes of CASP9?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
The immunogen of PB9332 anti-Caspase-9/CASP9 antibody is E.coli-derived human Caspase-9 recombinant protein (Position: E3-D228). Human Caspase-9 shares 63% amino acid (aa) sequence identity with mouse Caspase-9. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-24
Question
Is there a BSA free version of anti-Caspase-9/CASP9 antibody PB9332 available?
Verified Customer
Verified customer
Asked: 2019-01-28
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Caspase-9/CASP9 antibody PB9332 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-01-28
Question
We are currently using anti-Caspase-9/CASP9 antibody PB9332 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on feline tissues as well?
W. Banerjee
Verified customer
Asked: 2017-05-04
Answer
The anti-Caspase-9/CASP9 antibody (PB9332) has not been validated for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-05-04
Question
Does anti-Caspase-9/CASP9 antibody PB9332 work on goat WB with cervix carcinoma erythroleukemia?
A. Patel
Verified customer
Asked: 2016-05-18
Answer
Our lab technicians have not validated anti-Caspase-9/CASP9 antibody PB9332 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Caspase-9/CASP9 antibody PB9332 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat cervix carcinoma erythroleukemia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-05-18
Question
Does PB9332 anti-Caspase-9/CASP9 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
J. Krishna
Verified customer
Asked: 2014-09-03
Answer
It shows on the product datasheet, PB9332 anti-Caspase-9/CASP9 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2014-09-03
