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Product Info Summary
| SKU: | PA1595 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Caspase 9/CASP9 Antibody Picoband®
SKU/Catalog Number
PA1595
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Caspase 9/CASP9 Antibody catalog # PA1595. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Caspase 9/CASP9 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1595)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human Caspase-9.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1595 is reactive to CASP9 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
35 kDa
Calculated molecular weight
46281 MW
Background of Caspase-9
CASP9 (CASPASE9), also called APAF3, is an initiator caspase, encoded by the CASP9 gene. The CASP9 gene is mapped to chromosome 1p36.3-p36.1 by FISH. CASP9 is identified as a member of the caspase family that participates in caspase-3 activation in vitro. And it also regarded as the most upstream member of the apoptotic protease cascade that is triggered by cytochrome c and dATP. Its genomic coordinates (GRCh37) is 1:15,818,768-15,851,284. The crystal structure of CASP9 is complex with the BIR3 in an inhibitory domain of XIAP at 2.4-angstrom resolution and the CASP9 gene contains 9 exons and spans approximately 35 kb of genomic DNA. Caspase-9 and APAF1 bind to each other via their respective NH2-terminal CED-3 homologous domains in the presence of cytochrome c and dATP, an event that leads to caspase-9 activation. CASP9 activity increases dramatically upon association with the apoptosome complex. And the majority of Casp9 knockout mice died perinatally with a markedly enlarged and malformed cerebrum caused by reduced apoptosis during brain development.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1595 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: SMMC Whole Cell, MCF-7 Whole Cell, CEM Whole Cell, JURKAT Whole Cell, RAJI Whole Cell, HELA Whole Cell
IHC: Human Tonsil Tissue
Validation Images & Assay Conditions
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Anti-Caspase-9 antibody, PA1595, Western blotting
All lanes: Anti Caspase-9 (PA1595) at 0.5ug/ml
Lane 1: SMMC Whole Cell Lysate at 40ug
Lane 2: MCF-7 Whole Cell Lysate at 40ug
Lane 3: CEM Whole Cell Lysate at 40ug
Lane 4: JURKAT Whole Cell Lysate at 40ug
Lane 5: RAJI Whole Cell Lysate at 40ug
Lane 6: HELA Whole Cell Lysate at 40ug
Predicted bind size: 35KD
Observed bind size: 35KD
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Anti-Caspase-9 antibody, PA1595, IHC(P)
IHC(P): Human Tonsil Tissue
Protein Target Info & Infographic
Gene/Protein Information For CASP9 (Source: Uniprot.org, NCBI)
Gene Name
CASP9
Full Name
Caspase-9
Weight
46281 MW
Superfamily
peptidase C14A family
Alternative Names
Caspase-9;CASP-9;3.4.22.62;Apoptotic protease Mch-6;Apoptotic protease-activating factor 3;APAF-3;ICE-like apoptotic protease 6;ICE-LAP6;Caspase-9 subunit p35;Caspase-9 subunit p10;CASP9;MCH6; CASP9 APAF-3, APAF3, ICE-LAP6, MCH6, PPP1R56 caspase 9 caspase-9|ICE-like apoptotic protease 6|apoptotic protease MCH-6|apoptotic protease activating factor 3|caspase 9, apoptosis-related cysteine peptidase|protein phosphatase 1, regulatory subunit 56
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CASP9, check out the CASP9 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CASP9: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Caspase 9/CASP9 Antibody Picoband® (PA1595)
Hello CJ!
PA1595 has been cited in 19 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Li QY,Hou CZ,Yang LP,Chu XL,Wang Y,Zhang P,Zhao Y. Study on the Mechanism of Ginseng in the Treatment of Lung Adenocarcinoma Based on Network Pharmacology. Evid Based Complement Alternat Med.2020 Jul 31;2020:2658795. doi:10.1155/2020/2658795.PMID:32802118
Species: Human
PA1595 usage in article: APP:ICC, SAMPLE:A549 CELL, DILUTION:NA
In Vitro Effects of HAS-2 Gene Silencing on the Proliferation and Apoptosis of the MCF-7 Human Breast Cancer Cell Line
Chinese herbal medicine Yougui Pill reduces exogenous glucocorticoid-induced apoptosis in anterior pituitary cells
In vivo?and?in vitro?evaluation of the cytotoxic effects of Photosan-loaded hollow silica nanoparticles on liver cancer
Shi, C., Ma, Q., Ren, M., Liang, D., Yu, Q., & Luo, J. (2017). Antitumorpharmacological mechanism of the oral liquid of Poriacocos polysaccharide. Journal of Ethnopharmacology. Advance online publication. doi: 10.1016/j.jep.2017.07.003
Chang C, Liu Sp, Fang Ch, He Rs, Wang Z, Zhu Yq, Jiang Sw. Oncol Lett. 2013 Sep;6(3):699-704. Epub 2013 Jul 8. Effects Of Matrine On The Proliferation Of Ht29 Human Colon Cancer Cells And Its Antitumor Mechanism.
Yuan X, Sun Q, Ou Y, Wang S, Zhang W, Deng H, Wu X, Zhang L. Neural Regen Res. 2014 Apr 15;9(8):837-44. Doi: 10.4103/1673-5374.131600. Apoptosis Is An Obstacle To The Differentiation Of Adipose-Derived Stromal Cells Into Astrocytes.
Chen S, Peng H, Rowat A, Gao F, Zhang Z, Wang P, Zhang W, Wang X, Qu L. Brain Res. 2015 Aug 27;1618:205-11. Doi: 10.1016/J.Brainres.2015.05.027. Epub 2015 May 30. The Effect Of Concentration And Duration Of Normobaric Oxygen In Reducing Caspase-3 ...
Dai C, Li J, Tang S, Li J, Xiao X. Antimicrob Agents Chemother. 2014 Jul;58(7):4075-85. Doi: 10.1128/Aac.00070-14. Epub 2014 May 5. Colistin-Induced Nephrotoxicity In Mice Involves The Mitochondrial, Death Receptor, And Endoplasmic Reticulum Pathw...
Huang C, Lin Y, Su H, Ye D. Neurochem Res. 2015 Jan;40(1):27-35. Doi: 10.1007/S11064-014-1461-5. Epub 2014 Oct 25. Forsythiaside Protects Against Hydrogen Peroxide-Induced Oxidative Stress And Apoptosis In Pc12 Cell.
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Anti-Caspase 9/CASP9 Antibody Picoband®
Question
Do you have any data about PA1595 for use in IF with PFA fixed cells?
Verified customer
Asked: 2020-06-19
Answer
Our lab hasn't performed IF test with PFA fixed cells using the Anti-Caspase 9/CASP9 Antibody (PA1595). Please run a pilot test to see if it would work.
Boster Scientific Support
Answered: 2020-06-22
Question
We have observed staining in human adrenal tissue. Are there any suggestions? Is anti-Caspase 9/CASP9 antibody supposed to stain adrenal tissue positively?
Verified Customer
Verified customer
Asked: 2019-09-24
Answer
From literature adrenal tissue does express CASP9. From Uniprot.org, CASP9 is expressed in adrenal tissue, t-cell, stomach cancer, kidney, eye lymph, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have CASP9 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Eye, and Lymph, Pubmed ID: 15489334
Kidney, Pubmed ID: 14702039
Stomach cancer, Pubmed ID: 9890966
T-cell, Pubmed ID: 8900201
Boster Scientific Support
Answered: 2019-09-24
Question
We are currently using anti-Caspase 9/CASP9 antibody PA1595 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on dog tissues as well?
Verified Customer
Verified customer
Asked: 2018-12-31
Answer
The anti-Caspase 9/CASP9 antibody (PA1595) has not been validated for cross reactivity specifically with dog tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in dog you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-12-31
Question
We ordered your anti-Caspase 9/CASP9 antibody for IHC on kidney in the past. I am using human, and We are going to use the antibody for WB next. I was wanting to use examining kidney as well as cervix carcinoma in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2017-12-29
Answer
I have checked the website and datasheets of our anti-Caspase 9/CASP9 antibody and it seems that PA1595 has been validated on human in both IHC and WB. Thus PA1595 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2017-12-29
Question
I am interested in using your anti-Caspase 9/CASP9 antibody for activation of caspases through apoptosome-mediated cleavage studies. Has this antibody been tested with western blotting on smmc whole cell lysate? We would like to see some validation images before ordering.
C. Bhatt
Verified customer
Asked: 2014-10-16
Answer
We appreciate your inquiry. This PA1595 anti-Caspase 9/CASP9 antibody is tested on smmc whole cell lysate, cem whole cell lysate, jurkat whole cell lysate, raji whole cell lysate, hela whole cell lysate, human tonsil tissue. It is guaranteed to work for IHC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2014-10-16
