Anti-HLA-DPB1 Antibody Picoband®

HLA DPB1 antibody

Boster Bio Anti-HLA-DPB1 Antibody Picoband® catalog # A00487. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Cited in 1 publication(s).

Product Info Summary

SKU: A00487
Size: 100 μg/vial
Reactive Species: Human
Host: Rabbit
Application: IHC, WB

Product Name

Anti-HLA-DPB1 Antibody Picoband®

View all HLA DPB1 Antibodies

SKU/Catalog Number

A00487

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-HLA-DPB1 Antibody Picoband® catalog # A00487. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-HLA-DPB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00487)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human HLA-DPB1 recombinant protein (Position: R30-A258).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

A00487 is reactive to HLA-DPB1 in Human

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

29 kDa

Calculated molecular weight

29159 MW

Background of HLA DPB1

HLA class II histocompatibility antigen, DP (W2) beta chain is a protein that in humans is encoded by the HLA-DPB1 gene. HLA-DPB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DPA) and a beta chain (DPB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail. Within the DP molecule both the alpha chain and the beta chain contain the polymorphisms specifying the peptide binding specificities, resulting in up to 4 different molecules.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A00487 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry, 2-5μg/ml, Human

Positive Control

WB: human Raji whole cell, human Raji whole cell
IHC: human liver cancer tissue

Validation Images & Assay Conditions

Gene/Protein Information For HLA-DPB1 (Source: Uniprot.org, NCBI)

Gene Name

HLA-DPB1

Full Name

HLA class II histocompatibility antigen, DP beta 1 chain

Weight

29159 MW

Superfamily

MHC class II family

Alternative Names

HLA class II histocompatibility antigen, DP beta 1 chain;HLA class II histocompatibility antigen, DP (W4) beta chain;MHC class II antigen DPB1;HLA-DPB1;HLA-DP1B; HLA-DPB1 DPB1, HLA-DP, HLA-DP1B, HLA-DPB major histocompatibility complex, class II, DP beta 1 HLA class II histocompatibility , DP beta 1 chain|HLA class II histocompatibility , DP(W4) beta chain|HLA-DP histocompatibility type, beta-1 subunit|MHC HLA DPB1|MHC class II HLA-DP-beta-1|MHC class II DPB1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on HLA-DPB1, check out the HLA-DPB1 Infographic

HLA-DPB1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for HLA-DPB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

Hello CJ!

A00487 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Huang J, Zhu C, Zhang P, Zhu Q, Liu Y, Zhu Z, Wang M, Li W, Yang G, Dong N, Liu J, Chen L, Zhang Y, Yang R, Deng L, Fan J, Wang X, Liu J, Ma B, Fu Q, Wu K. Sci Rep. 2013;3:1114. Doi: 10.1038/Srep01114. Epub 2013 Jan 23. S100+ Cells: A New Neuro-Im...

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1 Customer Q&As for Anti-HLA-DPB1 Antibody Picoband®

Question

We are currently using anti-HLA-DPB1 antibody A00487 for rat tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?

B. Anderson

Verified customer

Asked: 2016-11-30

Answer

The anti-HLA-DPB1 antibody (A00487) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-11-30

Order DetailsPrice
A00487

100μg

$370
A00487-10ug

10μg sample (liquid)

$99
A00487-Biotin

100 μg Biotin conjugated

$570
A00487-Cy3

100 μg Cy3 conjugated

$570
A00487-Dylight488

100 μg Dylight488 conjugated

$570
A00487-Dylight550

100 μg Dylight550 conjugated

$570
A00487-Dylight594

100 μg Dylight594 conjugated

$570
A00487-FITC

100 μg FITC conjugated

$570
A00487-HRP

100 μg HRP conjugated

$570
A00487-APC

100 μg APC conjugated

$670
A00487-PE

100 μg PE conjugated

$670
A00487-iFluor647

100 μg iFluor647 conjugated

$670
A00487-carrier-free

Carrier Free

$370

More conjugates/formats

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A00487
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

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