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Product Info Summary
| SKU: | A00568 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | WB |
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Product info
Product Name
Anti-HLA Class II DRB1/HLA-DRB1 Antibody Picoband®
SKU/Catalog Number
A00568
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-HLA Class II DRB1/HLA-DRB1 Antibody Picoband® catalog # A00568. Tested in WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HLA Class II DRB1/HLA-DRB1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00568)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human HLA Class II DRB1/HLA-DRB1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00568 is reactive to HLA-DRB1 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
30 kDa
Calculated molecular weight
29.966kDa
Background of HLA-DRB1
HLA class II histocompatibility antigen, DRB1 beta chain is a protein that in humans is encoded by the HLA-DRB1 gene. This gene is mapped to 6p21.32. DRB1 encodes the most prevalent beta subunit of HLA-DR. Several alleles of DRB1 (shared epitope alleles) are associated with an increased incidence of rheumatoid arthritis. HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. It plays a central role in the immune system by presenting peptides derived from extracellular proteins to T helper cells.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00568 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Positive Control
WB: human Raji whole cell, human Raji whole cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of HLA Class II DRB1 using anti-HLA Class II DRB1 antibody (A00568).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Raji whole cell lysate,
Lane 2: human Raji whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA Class II DRB1 antigen affinity purified polyclonal antibody (Catalog # A00568) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for HLA Class II DRB1 at approximately 30KD. The expected band size for HLA Class II DRB1 is at 30KD.
Protein Target Info & Infographic
Gene/Protein Information For HLA-DRB1 (Source: Uniprot.org, NCBI)
Gene Name
HLA-DRB1
Full Name
HLA class II histocompatibility antigen, DRB1 beta chain
Weight
29.966kDa
Alternative Names
HLA class II histocompatibility antigen, DRB1-7 beta chain; MHC class II antigen DRB1*7; DR-7; DR7; HLA-DRB1 HLA-DRB1 DRB1, HLA-DR1B, HLA-DRB, SS1 major histocompatibility complex, class II, DR beta 1 major histocompatibility complex, class II, DR beta 1 precursor|HLA class II histocompatibility , DR-1 beta chain|MHC class II HLA-DR beta 1 chain|human leucocyte DRB1|lymphocyte DRB1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HLA-DRB1, check out the HLA-DRB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HLA-DRB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HLA Class II DRB1/HLA-DRB1 Antibody Picoband® (A00568)
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Customer Q&As
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1 Customer Q&As for Anti-HLA Class II DRB1/HLA-DRB1 Antibody Picoband®
Question
We are currently using anti-HLA Class II DRB1/HLA-DRB1 antibody A00568 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on canine tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-10
Answer
The anti-HLA Class II DRB1/HLA-DRB1 antibody (A00568) has not been validated for cross reactivity specifically with canine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in canine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-10
