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Product Info Summary
| SKU: | M04255-3 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, IHC, WB |
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Product info
Product Name
Anti-Calretinin Antibody
View all Calretinin Antibodies
SKU/Catalog Number
M04255-3
Size
100ul
Description
Boster Bio Anti-Calretinin Antibody catalog # M04255-3. Tested in IF, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Calretinin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04255-3)
Host
Rabbit
Contents
Supplied as an aliquot of serum plus 5mM NaN3
Clonality
Polyclonal
Immunogen
Full-length recombinant human calretinin protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04255-3 is reactive to CALB2 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
31540 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04255-3 is guaranteed for IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:5,000-10,000 IF/ICC or IHC: 1:5,000-1:10,000
Validation Images & Assay Conditions
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Western blot analysis of different tissue lysates probed with rabbit pAb to calretinin, M04255-3, dilution 1:10,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, and [6] cow spinal cord. A band at 29kDa corresponds to the calretinin protein.
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Immunofluorescent analysis of rat cerebellum section stained with rabbit pAb to calretinin, M04255-3, dilution 1:5,000 in red, and costained with mouse mAb to calbindin, 1:1,000 in green. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45M, and free-floating sections were stained with the above antibodies. The calretinin antibody stains interneurons predominantly in the molecular layer, while the calbindin antibody strongly labels the dendrites and perikarya of Purkinje cells in the molecular layer of the cerebellum.
Protein Target Info & Infographic
Gene/Protein Information For CALB2 (Source: Uniprot.org, NCBI)
Gene Name
CALB2
Full Name
Calretinin
Weight
31540 MW
Superfamily
calbindin family
Alternative Names
Calretinin;CR;29 kDa calbindin;CALB2;CAB29; CALB2 CAB29, CAL2, CR calbindin 2 calretinin|29 kDa calbindin|calbindin 2, (29kD, calretinin)|calbindin D29K|testicular secretory protein Li 8
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CALB2, check out the CALB2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CALB2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Calretinin Antibody (M04255-3)
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