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Product Info Summary
| SKU: | PA1922 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Monkey, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, WB |
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Product info
Product Name
Anti-MEK7/MAP2K7 Antibody Picoband®
SKU/Catalog Number
PA1922
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-MEK7/MAP2K7 Antibody catalog # PA1922. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-MEK7/MAP2K7 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1922)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human MEK7, identical to the related rat and mouse sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1922 is reactive to MAP2K7 in Human, Monkey, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
47 kDa
Calculated molecular weight
47485 MW
Background of MKK7/MEK7
MAP2K7 (Mitogen-activated protein kinase kinase 7), also known as MAP kinase kinase 7, MAPKK7, JNKK2, PRKMK7 or MKK7, is an enzyme that in humans is encoded by the MAP2K7 gene. This protein is a member of the mitogen-activated protein kinase kinase family. The MKK7 protein exists as six different isoforms with three possible N-termini (alpha, beta, and gamma isoforms) and two possible C-termini (1 and 2 isoforms). Schramek et al. (2011) showed that the doxorubicin-mediated DNA damage response in human A549 lung carcinoma cells caused rapid phosphorylation and upregulation of p53 (TP53). MKK7 knockdown reduced p53 phosphorylation, delayed p53 upregulation, and interfered with cell cycle arrest at G2/M. MKK7 was activated in primary lung tumors, and tumors with a p53 mutation showed even higher MKK7 phosphorylation. Schramek et al. (2011) concluded that MKK7 exerts its tumor suppressive function through p53.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1922 is guaranteed for Flow Cytometry, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
Positive Control
WB: human Hela whole cell, monkey COS-7 whole cell, rat skeletal muscle tissue, mouse skeletal muscle tissue
FCM: Jurkat cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of MEK7/MAP2K7 using anti-MEK7/MAP2K7 antibody (PA1922).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: monkey COS-7 whole cell lysates,
Lane 3: rat skeletal muscle tissue lysates,
Lane 4: mouse skeletal muscle tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MEK7/MAP2K7 antigen affinity purified polyclonal antibody (Catalog # PA1922) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for MEK7/MAP2K7 at approximately 47 kDa. The expected band size for MEK7/MAP2K7 is at 47 kDa.
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Figure 2. Flow Cytometry analysis of Jurkat cells using anti-MEK7/MAP2K7 antibody (PA1922).
Overlay histogram showing Jurkat cells stained with PA1922 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MEK7/MAP2K7 Antibody (PA1922, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For MAP2K7 (Source: Uniprot.org, NCBI)
Gene Name
MAP2K7
Full Name
Dual specificity mitogen-activated protein kinase kinase 7
Weight
47485 MW
Superfamily
protein kinase superfamily
Alternative Names
Dual specificity mitogen-activated protein kinase kinase 7;MAP kinase kinase 7;MAPKK 7;2.7.12.2;JNK-activating kinase 2;MAPK/ERK kinase 7;MEK 7;Stress-activated protein kinase kinase 4;SAPK kinase 4;SAPKK-4;SAPKK4;c-Jun N-terminal kinase kinase 2;JNK kinase 2;JNKK 2;MAP2K7;JNKK2, MEK7, MKK7, PRKMK7, SKK4; MAP2K7 JNKK2, MAPKK7, MEK, MEK 7, MKK7, PRKMK7, SAPKK-4, SAPKK4 mitogen-activated protein kinase kinase 7 dual specificity mitogen-activated protein kinase kinase 7|JNK-activating kinase 2|MAP kinase kinase 7|MAPK/ERK kinase 7|SAPK kinase 4|c-Jun N-terminal kinase kinase 2|stress-activated protein kinase kinase 4
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on MAP2K7, check out the MAP2K7 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for MAP2K7: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-MEK7/MAP2K7 Antibody Picoband® (PA1922)
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Customer Q&As
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6 Customer Q&As for Anti-MEK7/MAP2K7 Antibody Picoband®
Question
Is a blocking peptide available for product anti-MEK7/MAP2K7 antibody (PA1922)?
Verified Customer
Verified customer
Asked: 2020-02-04
Answer
We do provide the blocking peptide for product anti-MEK7/MAP2K7 antibody (PA1922). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-02-04
Question
Would PA1922 anti-MEK7/MAP2K7 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-11-21
Answer
You can see on the product datasheet, PA1922 anti-MEK7/MAP2K7 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-11-21
Question
Would anti-MEK7/MAP2K7 antibody PA1922 work for WB with brain?
Verified Customer
Verified customer
Asked: 2018-06-12
Answer
According to the expression profile of brain, MAP2K7 is highly expressed in brain. So, it is likely that anti-MEK7/MAP2K7 antibody PA1922 will work for WB with brain.
Boster Scientific Support
Answered: 2018-06-12
Question
I have a question about product PA1922, anti-MEK7/MAP2K7 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2017-06-01
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1922 anti-MEK7/MAP2K7 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-06-01
Question
We are interested in to test anti-MEK7/MAP2K7 antibody PA1922 on rat brain for research purposes, then I may be interested in using anti-MEK7/MAP2K7 antibody PA1922 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
B. Mangal
Verified customer
Asked: 2014-11-24
Answer
The products we sell, including anti-MEK7/MAP2K7 antibody PA1922, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2014-11-24
Question
Is this PA1922 anti-MEK7/MAP2K7 antibody reactive to the isotypes of MAP2K7?
S. Carter
Verified customer
Asked: 2013-01-31
Answer
The immunogen of PA1922 anti-MEK7/MAP2K7 antibody is A synthetic peptide corresponding to a sequence at the N-terminus of human MEK7(24-40aa ARRRIDLNLDISPQRPR), identical to the related rat and mouse sequences. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2013-01-31
