Anti-C Reactive Protein CRP Rabbit Monoclonal Antibody

C-Reactive Protein/CRP antibody

Boster Bio Anti-C Reactive Protein CRP Rabbit Monoclonal Antibody catalog # M00249. Tested in WB, IHC, IP applications. This antibody reacts with Human.

Product Info Summary

SKU: M00249
Size: 100 μl
Reactive Species: Human
Host: Rabbit
Application: IP, IHC, WB

Product Name

Anti-C Reactive Protein CRP Rabbit Monoclonal Antibody

View all C-Reactive Protein/CRP Antibodies

SKU/Catalog Number

M00249

Size

100 μl

Form

Liquid

Description

Boster Bio Anti-C Reactive Protein CRP Rabbit Monoclonal Antibody catalog # M00249. Tested in WB, IHC, IP applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-C Reactive Protein CRP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00249)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

Clonality

Monoclonal

Clone Number

CFI-3

Isotype

Rabbit IgG

Immunogen

A synthesized peptide derived from human C Reactive Protein

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M00249 is reactive to CRP in Human

Applications

M00249 is guaranteed for IP, IHC, WB Boster Guarantee

Observed Molecular Weight

35-60 kDa

Calculated molecular weight

25039 MW

Background of C-Reactive Protein/CRP

C3 plays a central role in the activation of the complement system. Its processing by C3 convertase is the central reaction in both classical and alternative complement pathways. After activation C3b can bind covalently, via its reactive thioester, to cell surface carbohydrates or immune aggregates.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconstitution

Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1000-1:2000
IHC 1:500-1:1000
IP 1:50

Validation Images & Assay Conditions

Gene/Protein Information For CRP (Source: Uniprot.org, NCBI)

Gene Name

CRP

Full Name

C-reactive protein

Weight

25039 MW

Superfamily

pentraxin family

Alternative Names

C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895 CRP PTX1 C-reactive protein C-reactive protein|C-reactive protein, pentraxin-related|pentraxin 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on CRP, check out the CRP Infographic

CRP infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for CRP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-C Reactive Protein CRP Rabbit Monoclonal Antibody

Question

We are currently using anti-C Reactive Protein Rabbit Monoclonal antibody M00249 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?

Verified Customer

Verified customer

Asked: 2019-10-03

Answer

The anti-C Reactive Protein Rabbit Monoclonal antibody (M00249) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-10-03

Question

We have tried in the past anti-C Reactive Protein Rabbit Monoclonal antibody for IP on liver in a previous project. I am using human, and We intend to use the antibody for IHC next. We need examining liver as well as right lobe of liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?

L. Mitchell

Verified customer

Asked: 2019-07-15

Answer

I looked at the website and datasheets of our anti-C Reactive Protein Rabbit Monoclonal antibody and it appears that M00249 has been validated on human in both IP and IHC. Thus M00249 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2019-07-15

Question

Our lab were happy with the WB result of your anti-C Reactive Protein Rabbit Monoclonal antibody. However we have seen positive staining in liver secreted. using this antibody. Is that expected? Could you tell me where is CRP supposed to be expressed?

Verified Customer

Verified customer

Asked: 2018-05-18

Answer

Based on literature, liver does express CRP. Generally CRP expresses in secreted. Regarding which tissues have CRP expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 15489334
Urinary bladder, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2018-05-18

Question

We have seen staining in human right lobe of liver. Are there any suggestions? Is anti-C Reactive Protein Rabbit Monoclonal antibody supposed to stain right lobe of liver positively?

K. Krishna

Verified customer

Asked: 2013-02-08

Answer

Based on literature right lobe of liver does express CRP. Based on Uniprot.org, CRP is expressed in right lobe of liver, liver, urinary bladder, among other tissues. Regarding which tissues have CRP expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 15489334
Urinary bladder, Pubmed ID: 14702039

Boster Scientific Support

Answered: 2013-02-08

Order DetailsPrice
M00249

100μL

$370
M00249-30ul

30μL sample

$99
M00249-carrier-free-1ml

Carrier Free

$2590

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*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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