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Product Info Summary
| SKU: | M00249 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IP, IHC, WB |
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Product info
Product Name
Anti-C Reactive Protein CRP Rabbit Monoclonal Antibody
View all C-Reactive Protein/CRP Antibodies
SKU/Catalog Number
M00249
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-C Reactive Protein CRP Rabbit Monoclonal Antibody catalog # M00249. Tested in WB, IHC, IP applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-C Reactive Protein CRP Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00249)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
CFI-3
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human C Reactive Protein
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00249 is reactive to CRP in Human
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
35-60 kDa
Calculated molecular weight
25039 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00249 is guaranteed for IP, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:500-1:1000
IP 1:50
Positive Control
IHC: human liver tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:2K dilution for 1 hour at room temperature.
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Western blot analysis of CRP expression in Etoposide treated 293 whole cell lysates.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Immunohistochemical analysis of paraffin-embedded human liver, using C Reactive Protein Antibody.
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
Protein Target Info & Infographic
Gene/Protein Information For CRP (Source: Uniprot.org, NCBI)
Gene Name
CRP
Full Name
C-reactive protein
Weight
25039 MW
Superfamily
pentraxin family
Alternative Names
C-reactive protein;C-reactive protein (1-205);CRP;PTX1; CRP PTX1 C-reactive protein C-reactive protein|C-reactive protein, pentraxin-related|pentraxin 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CRP, check out the CRP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CRP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-C Reactive Protein CRP Rabbit Monoclonal Antibody (M00249)
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No publications found for M00249
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Anti-C Reactive Protein CRP Rabbit Monoclonal Antibody
Question
We are currently using anti-C Reactive Protein Rabbit Monoclonal antibody M00249 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
The anti-C Reactive Protein Rabbit Monoclonal antibody (M00249) has not been tested for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-03
Question
We have tried in the past anti-C Reactive Protein Rabbit Monoclonal antibody for IP on liver in a previous project. I am using human, and We intend to use the antibody for IHC next. We need examining liver as well as right lobe of liver in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
L. Mitchell
Verified customer
Asked: 2019-07-15
Answer
I looked at the website and datasheets of our anti-C Reactive Protein Rabbit Monoclonal antibody and it appears that M00249 has been validated on human in both IP and IHC. Thus M00249 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-07-15
Question
Our lab were happy with the WB result of your anti-C Reactive Protein Rabbit Monoclonal antibody. However we have seen positive staining in liver secreted. using this antibody. Is that expected? Could you tell me where is CRP supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-05-18
Answer
Based on literature, liver does express CRP. Generally CRP expresses in secreted. Regarding which tissues have CRP expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 15489334
Urinary bladder, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-05-18
Question
We have seen staining in human right lobe of liver. Are there any suggestions? Is anti-C Reactive Protein Rabbit Monoclonal antibody supposed to stain right lobe of liver positively?
K. Krishna
Verified customer
Asked: 2013-02-08
Answer
Based on literature right lobe of liver does express CRP. Based on Uniprot.org, CRP is expressed in right lobe of liver, liver, urinary bladder, among other tissues. Regarding which tissues have CRP expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 15489334
Urinary bladder, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2013-02-08
