Key=store_id Key=entity_id, value=36249 Key=attribute_set_id, value=4 Key=type_id, value=simple Key=sku, value=A04345 Key=has_options, value=1 Key=required_options, value=1 Key=created_at, value=2017-05-27 07:29:42 Key=updated_at, value=2024-09-26 00:55:24 Key=mst_search_weight, value=0 Key=status, value=Enabled Key=name, value=Anti-VIP Receptor 1/VIPR1 Antibody Picoband® Key=cj_search_index Key=publication_count, value=0 Key=qty_in_warehouses Key=is_recurring, value=No Key=visibility, value=Catalog, Search Key=tax_class_id, value=Taxable Goods Key=featured, value=No Key=featured_image, value=Yes Key=fb_product, value= Key=custom_product_page, value=No Key=free_shipping, value=No Key=template, value=antibodies Key=host, value=Rabbit Key=googleshopping_exclude, value=No Key=yoast_keyword_score, value=-1175 Key=yoast_content_score, value=30 Key=badge, value=free-antibody-validation Key=price, value=370.0000 Key=weight, value=1.0000 Key=meta_title, value=VIP Receptor 1/VIPR1 Antibody Key=meta_description, value=Boster Bio Anti-VIP Receptor 1/VIPR1 Antibody Picoband® catalog # A04345. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. Supplied as 100μg/vial in Lyophilized form antibody. Key=image, value=/a/0/a04345-vip_receptor_1-primary-antibodies-wb-testing-1.jpg Key=small_image, value=/a/0/a04345-vip_receptor_1-primary-antibodies-wb-testing-1.jpg Key=thumbnail, value=/a/0/a04345-vip_receptor_1-primary-antibodies-wb-testing-1.jpg Key=url_key, value=anti-vip-receptor-1-picoband-trade-antibody-a04345-boster Key=url_path, value=anti-vip-receptor-1-picoband-trade-antibody-a04345-boster Key=options_container, value=Product Info Column Key=image_label, value=Figure 1. Western blot analysis of VIP Receptor 1 using anti-VIP Receptor 1 antibody (A04345). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded w Key=small_image_label, value=Figure 1. Western blot analysis of VIP Receptor 1 using anti-VIP Receptor 1 antibody (A04345). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded w Key=thumbnail_label, value=Figure 1. Western blot analysis of VIP Receptor 1 using anti-VIP Receptor 1 antibody (A04345). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded w Key=msrp_display_actual_price_type, value= Key=gift_message_available, value=No Key=size, value=100 μg/vial Key=gene_full_name, value=vasoactive intestinal peptide receptor 1 Key=uniprot_id, value=P32241 Key=reconstitution, value=Add 0.2ml of distilled water will yield a concentration of 500ug/ml. Key=sensitivity Key=applications, value=IHC, WB Key=reactivity, value=Human, Mouse, Rat Key=form, value=Lyophilized Key=concentration, value=Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. Key=cross_reactivity, value=No cross-reactivity with other proteins. Key=recommended_detection_systems, value=Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P). Key=predicted_reactivity Key=source_company, value=Boster A04345 Key=isotype, value=Rabbit IgG Key=sample_size_available, value=30ug for $99, contact us for details Key=related_product_skus, value=B0001-5,AR1156,AR0146 Key=product_category, value=Primary Antibodies Key=research_category, value=Adaptive Immunity, B Cells, Hematopoietic Progenitors, Immunoglobulins, Immunology, Lymphoid, Receptors, Stem Cells Key=clonality, value=Polyclonal Key=swatch_image, value=/a/0/a04341-1-gfpt1-primary-antibodies-wb-testing-1.jpg Key=rating_value, value=100 Key=review_count, value=6 Key=phospho_site Key=mp_exclude_sitemap, value=Yes Key=yoast_facebook_image, value=/a/0/a04341-1-gfpt1-primary-antibodies-wb-testing-1.jpg Key=yoast_twitter_image, value=/a/0/a04341-1-gfpt1-primary-antibodies-wb-testing-1.jpg Key=cj_related_products, value=Observation Of Neuromuscular Block:PA2059^Schistosomiasis:EK1258^T Cell Migration:FEK0494|FEK0495|FEK0496|PB9079 Key=out_of_stock_until_date, value=Nov 16, 2019 Key=description, value=Boster Bio Anti-VIP Receptor 1/VIPR1 Antibody Picoband® catalog # A04345. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Key=short_description, value=Boster Bio Anti-VIP Receptor 1/VIPR1 Antibody Picoband® catalog # A04345. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance. Key=meta_keyword, value=Anti-VIP Receptor 1/VIPR1 Antibody Picoband® Key=contents, value=Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. Key=background, value=VIPR1 (Vasoactive intestinal polypeptide receptor 1),also known as VIPR,HVR1, is a protein that in humans is encoded by the VIPR1 gene. Distinct subsets of neural, respiratory, gastrointestinal, and immune cells bear specific high-affinity G protein-coupled receptors for VIP, such as VIPR1. The VIPR1 gene is mapped on 3p22.1. The VIPR1 gene was found to span approximately 22 kb and to be comprised of 13 exons (ranging from 42 to 1,400 bp) and 12 introns (ranging from 0.3 to 6.1 kb). One encodes a VIP receptor consisting of 460 amino acids and having 7 putative transmembrane domains, as do other G protein-coupled receptors. Patients with idiopathic achalasia show a significant difference in the distribution of SNPs affecting VIPR1. Key=gene_name, value=VIPR1 Key=synonyms, value=Vasoactive intestinal polypeptide receptor 1; VIP-R-1; Pituitary adenylate cyclase-activating polypeptide type II receptor; PACAP type II receptor; PACAP-R-2; PACAP-R2; VPAC1; VIPR1 Key=application_details, value=Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Key=subcellular_localization, value= Cell membrane; Multi-pass membrane protein. Key=tissue_specificity, value= In lung, HT-29 colonic epithelial cells, Raji B-lymphoblasts. Lesser extent in brain, heart, kidney, liver and placenta. Not expressed in CD4+ or CD8+ T-cells. Expressed in the T-cell lines HARRIS, HuT 78, Jurkat and SUP-T1, but not in the T- cell lines Peer, MOLT-4, HSB and YT. Key=protein_function, value= PACAP-27 > PACAP-38. Key=immunogen, value=A synthetic peptide corresponding to a sequence at the C-terminus of human VIP Receptor 1, which shares 84% amino acid (aa) sequence identity with both mouse and rat VIP Receptor 1. Key=storage, value=Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. Key=custom_attribute_1, value=52 kDa Key=custom_attribute_2, value=WB: human A431 cell, human PANC-1 cell Key=options Key=media_gallery Key=extension_attributes Key=tier_price Key=tier_price_changed Key=category_ids Key=quantity_and_stock_status Key=is_salable Key=website_ids Key=request_path Key=rating_summary Key=_cache_instance_store_filter Key=media_gallery_images
Anti-VIP Receptor 1/VIPR1 Antibody Picoband®
VIPR1/VPAC1 antibody
Boster Bio Anti-VIP Receptor 1/VIPR1 Antibody Picoband® catalog # A04345. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Boster Bio Anti-VIP Receptor 1/VIPR1 Antibody Picoband® catalog # A04345. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-VIP Receptor 1/VIPR1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04345)
A synthetic peptide corresponding to a sequence at the C-terminus of human VIP Receptor 1, which shares 84% amino acid (aa) sequence identity with both mouse and rat VIP Receptor 1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A04345 is reactive to VIPR1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
52 kDa
Calculated molecular weight
51.547kDa
Background of VIPR1/VPAC1
VIPR1 (Vasoactive intestinal polypeptide receptor 1),also known as VIPR,HVR1, is a protein that in humans is encoded by the VIPR1 gene. Distinct subsets of neural, respiratory, gastrointestinal, and immune cells bear specific high-affinity G protein-coupled receptors for VIP, such as VIPR1. The VIPR1 gene is mapped on 3p22.1. The VIPR1 gene was found to span approximately 22 kb and to be comprised of 13 exons (ranging from 42 to 1,400 bp) and 12 introns (ranging from 0.3 to 6.1 kb). One encodes a VIP receptor consisting of 460 amino acids and having 7 putative transmembrane domains, as do other G protein-coupled receptors. Patients with idiopathic achalasia show a significant difference in the distribution of SNPs affecting VIPR1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Positive Control
WB: human A431 cell, human PANC-1 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of VIP Receptor 1 using anti-VIP Receptor 1 antibody (A04345). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A431 cell lysates, Lane 2: human PANC-1 cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-VIP Receptor 1 antigen affinity purified polyclonal antibody (Catalog # A04345) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for VIP Receptor 1 at approximately 52KD. The expected band size for VIP Receptor 1 is at 52KD.
Gene/Protein Information For VIPR1 (Source: Uniprot.org, NCBI)
Gene Name
VIPR1
Full Name
Vasoactive intestinal polypeptide receptor 1
Weight
51.547kDa
Superfamily
G-protein coupled receptor 2 family
Alternative Names
Vasoactive intestinal polypeptide receptor 1; VIP-R-1; Pituitary adenylate cyclase-activating polypeptide type II receptor; PACAP type II receptor; PACAP-R-2; PACAP-R2; VPAC1; VIPR1 VIPR1 HVR1, II, PACAP-R-2, PACAP-R2, RDC1, V1RG, VAPC1, VIP-R-1, VIPR, VIRG, VPAC1, VPAC1R, VPCAP1R vasoactive intestinal peptide receptor 1 vasoactive intestinal polypeptide receptor 1|PACAP type II receptor|VIP and PACAP receptor 1|VIP receptor, type I|VPAC1 receptor|pituitary adenylate cyclase activating polypeptide receptor, type II|type 1 vasoactive intestinal peptide receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".
For more info on VIPR1, check out the VIPR1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for VIPR1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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