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Product Info Summary
| SKU: | M00776-1 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Bub1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00776-1
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-Bub1 Rabbit Monoclonal Antibody catalog # M00776-1. Tested in WB, IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bub1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00776-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
19B32
Isotype
IgG
Immunogen
A synthesized peptide derived from human Bub1
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00776-1 is reactive to BUB1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
15 kDa
Calculated molecular weight
53977 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00776-1 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:1000
IHC 1:50-1:200
Positive Control
WB: K562 cell
Validation Images & Assay Conditions
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Western blot analysis of Bub1 expression in K562 cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For BUB1 (Source: Uniprot.org, NCBI)
Gene Name
BUB1
Full Name
Mitotic checkpoint serine/threonine-protein kinase BUB1
Weight
53977 MW
Superfamily
protein kinase superfamily
Alternative Names
Stromelysin-1;SL-1;3.4.24.17;Matrix metalloproteinase-3;MMP-3;Transin-1;MMP3;STMY1; BUB1 BUB1AL, hBUB1, BUB1 BUB1 mitotic checkpoint serine/threonine kinase mitotic checkpoint serine/threonine-protein kinase BUB1|BUB1 budding uninhibited by benzimidazoles 1 homolog|budding uninhibited by benzimidazoles 1 homolog|mitotic spindle checkpoint kinase|putative serine/threonine-protein kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BUB1, check out the BUB1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BUB1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bub1 Rabbit Monoclonal Antibody (M00776-1)
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