Anti-Bone Sialoprotein/IBSP Antibody Picoband®

Product Name

Anti-Bone Sialoprotein/IBSP Antibody Picoband®

View all IBSP/Sialoprotein II Antibodies

SKU/Catalog Number

PA1505

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Bone Sialoprotein/IBSP Antibody catalog # PA1505. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Bone Sialoprotein/IBSP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1505)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human Bone Sialoprotein, different from the related rat and mouse sequences by three amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1505 is reactive to IBSP in Human, Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

37 kDa

Calculated molecular weight

35148 MW

Background of IBSP/Sialoprotein II

BSP is also known as IBSP (integrin-binding sialoprotein). The protein encoded by this gene is a major structural protein of the bone matrix. Bone sialoprotein is an acidic glycoprotein of approximately 70 kD that undergoes extensive posttranslational modifications. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. The BSP gene is mapped to 4q22.1.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

View more details

Applications

PA1505 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse

Positive Control

IHC: Human Osteosarcoma Tissue

Validation Images & Assay Conditions

Gene/Protein Information For IBSP (Source: Uniprot.org, NCBI)

Alternative Names

Bone sialoprotein 2;Bone sialoprotein II;BSP II;Cell-binding sialoprotein;Integrin-binding sialoprotein;IBSP;BNSP; IBSP BNSP, BSP, BSP-II, SP-II integrin binding sialoprotein bone sialoprotein 2|BSP II|bone sialoprotein II|cell-binding sialoprotein

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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