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Product Info Summary
| SKU: | PA1887 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Bone Sialoprotein/IBSP Antibody Picoband®
View all IBSP/Sialoprotein II Antibodies
SKU/Catalog Number
PA1887
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Bone Sialoprotein/IBSP Antibody catalog # PA1887. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bone Sialoprotein/IBSP Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1887)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg Thimerosal, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of mouse Bone Sialoprotein, different from the related rat sequence by one amino acid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1887 is reactive to Ibsp in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
65 kDa
Calculated molecular weight
35734 MW
Background of IBSP/Sialoprotein II
IBSP (integrin-binding sialoprotein) is also known as BSP. The protein encoded by this gene is a major structural protein of the bone matrix. Bone sialoprotein is an acidic glycoprotein of approximately 70 kD that undergoes extensive posttranslational modifications. It constitutes approximately 12% of the noncollagenous proteins in human bone and is synthesized by skeletal-associated cell types, including hypertrophic chondrocytes, osteoblasts, osteocytes, and osteoclasts. The only extraskeletal site of its synthesis is the trophoblast. This protein binds to calcium and hydroxyapatite via its acidic amino acid clusters, and mediates cell attachment through an RGD sequence that recognizes the vitronectin receptor. The BSP gene is mapped to 4q22.1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1887 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Positive Control
WB: rat cartilage tissue, mouse cartilage tissue
IHC: mouse pancreas tissue, rat liver tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IBSP using anti-IBSP antibody (PA1887).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat cartilage tissue lysates,
Lane 2: mouse cartilage tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IBSP antigen affinity purified polyclonal antibody (Catalog # PA1887) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IBSP at approximately 65 kDa. The expected band size for IBSP is at 35 kDa.
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Figure 2. IHC analysis of IBSP using anti-IBSP antibody (PA1887).
IBSP was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-IBSP Antibody (PA1887) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Click image to see more details
Figure 3. IHC analysis of Bone Sialoprotein using anti-Bone Sialoprotein antibody (PA1887).
Bone Sialoprotein was detected in paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Bone Sialoprotein Antibody (PA1887) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Ibsp (Source: Uniprot.org, NCBI)
Gene Name
Ibsp
Full Name
Bone sialoprotein 2
Weight
35734 MW
Alternative Names
Bone sialoprotein 2;Bone sialoprotein II;BSP II;Cell-binding sialoprotein;Integrin-binding sialoprotein;Ibsp; IBSP BNSP, BSP, BSP-II, SP-II integrin binding sialoprotein bone sialoprotein 2|BSP II|bone sialoprotein II|cell-binding sialoprotein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Ibsp, check out the Ibsp Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Ibsp: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bone Sialoprotein/IBSP Antibody Picoband® (PA1887)
Hello CJ!
PA1887 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effect of kidney-reinforcing and marrow-beneficial Chinese medicine on bone metabolism-related factors following spinal cord injury in rats
The Osteogenic Potential of Mesoporous Bioglasses/Silk and Non-Mesoporous Bioglasses/Silk Scaffolds in Ovariectomized Rats: In vitro and In vivo Evaluation
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Customer Q&As
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7 Customer Q&As for Anti-Bone Sialoprotein/IBSP Antibody Picoband®
Question
Is a blocking peptide available for product anti-Bone Sialoprotein/IBSP antibody (PA1887)?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
We do provide the blocking peptide for product anti-Bone Sialoprotein/IBSP antibody (PA1887). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-01-30
Question
We are currently using anti-Bone Sialoprotein/IBSP antibody PA1887 for mouse tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2019-09-26
Answer
The anti-Bone Sialoprotein/IBSP antibody (PA1887) has not been validated for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-26
Question
I was wanting to use your anti-Bone Sialoprotein/IBSP antibody for IHC-P for rat tibia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat tibia identification?
Verified Customer
Verified customer
Asked: 2019-06-06
Answer
You can see on the product datasheet, PA1887 anti-Bone Sialoprotein/IBSP antibody has been tested for IHC-P, WB on mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat tibia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-06
Question
Would anti-Bone Sialoprotein/IBSP antibody PA1887 work for IHC-P with tibia?
Verified Customer
Verified customer
Asked: 2018-07-10
Answer
According to the expression profile of tibia, IBSP is highly expressed in tibia. So, it is likely that anti-Bone Sialoprotein/IBSP antibody PA1887 will work for IHC-P with tibia.
Boster Scientific Support
Answered: 2018-07-10
Question
Here is the WB image, lot number and protocol we used for tibia using anti-Bone Sialoprotein/IBSP antibody PA1887. Please let me know if you require anything else.
M. Anderson
Verified customer
Asked: 2017-08-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-08-17
Question
Does anti-Bone Sialoprotein/IBSP antibody PA1887 work on goat WB with tibia?
Verified Customer
Verified customer
Asked: 2017-08-09
Answer
Our lab technicians have not tested anti-Bone Sialoprotein/IBSP antibody PA1887 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Bone Sialoprotein/IBSP antibody PA1887 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat tibia in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-08-09
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for tibia using anti-Bone Sialoprotein/IBSP antibody PA1887. Let me know if you need anything else.
W. Brown
Verified customer
Asked: 2014-10-21
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-10-21
