Anti-BOB1/POU2AF1 Antibody Picoband®

Product Name

Anti-BOB1/POU2AF1 Antibody Picoband®

View all BOB1 Antibodies

SKU/Catalog Number

PA1506

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-BOB1/POU2AF1 Antibody catalog # PA1506. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BOB1/POU2AF1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1506)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the C-terminus of mouse BOB1, different from the related rat sequence by one amino acid, and from human sequence by four amino acids.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1506 is reactive to Pou2af1 in Mouse, Rat

Reconstitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Observed Molecular Weight

37 kDa

Calculated molecular weight

27692 MW

Background of BOB1

POU2AF1 (POU domain class 2-associating factor 1), also known as OBF1, BOB1 and OCAB, is a protein that in humans is encoded by the POU2AF1 gene. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Casellas et al. (2002) reported that OCAB is essential for V (D)J recombination of a subset of Ig-kappa genes. They showed that OCAB modulates recombination by directly enhancing Ig-kappa gene transcription in vivo. Siegel et al. (2006) suggested that, together with dysregulation of other OCAB target genes, altered regulation of SYK may help explain the magnitude of defects observed in B-cell development, including the pre-B1-to-pre-B2 transition, and immune responses in Ocab -/- mice.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

PA1506 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse, By Heat
Western blot, 0.1-0.5μg/ml, Mouse

Positive Control

WB: Rat Spleen Tissue
IHC: Rat Tonsil Tissue

Validation Images & Assay Conditions

Gene/Protein Information For Pou2af1 (Source: Uniprot.org, NCBI)

Alternative Names

POU domain class 2-associating factor 1;B-cell-specific coactivator OBF-1;BOB-1;BOB1;OCA-B;OCT-binding factor 1;Pou2af1;Obf-1; POU2AF1 BOB1, OBF-1, OBF1, OCAB POU class 2 homeobox associating factor 1 POU domain class 2-associating factor 1|B-cell-specific coactivator OBF-1|BOB-1|OCA-B|OCT-binding factor 1|POU class 2 associating factor 1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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