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Product Info Summary
| SKU: | A00607-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, WB |
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Product info
Product Name
Anti-IL23 Receptor/Il23r Antibody Picoband®
SKU/Catalog Number
A00607-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-IL23 Receptor/Il23r Antibody Picoband® catalog # A00607-1. Tested in ELISA, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-IL23 Receptor/Il23r Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00607-1)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived mouse IL23 Receptor recombinant protein (Position: I25-D233).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00607-1 is reactive to Il23r in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
58 kDa
Calculated molecular weight
71.722kDa
Background of IL-23R
Interleukin 23 receptor is a type I cytokine receptor. IL23R is its human gene. The protein encoded by this gene is a subunit of the receptor for IL23A/IL23. This protein pairs with the receptor molecule IL12RB1/IL12Rbeta1, and both are required for IL23A signaling. This protein associates constitutively with Janus kinase 2 (JAK2), and also binds to transcription activator STAT3 in a ligand-dependent manner.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00607-1 is guaranteed for ELISA, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
ELISA (Cap), 1-5μg/ml
Positive Control
WB: mouse brain tissue, mouse liver tissue, mouse kidney tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of IL23 Receptor using anti-IL23 Receptor antibody (A00607-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: mouse brain tissue lysates,
Lane 2: mouse liver tissue lysates,
Lane 3: mouse kidney tissue lysates.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IL23 Receptor antigen affinity purified polyclonal antibody (Catalog # A00607-1) at 0.5 ug/mL overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IL23 Receptor at approximately 58KD. The expected band size for IL23 Receptor is at 72KD.
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Figure 2. Sandwich ELISA - Recombinant mouse IL23 Receptor/Il23r protein standard curve.
Use in combination with reagents from Mouse IL23 Receptor/Il23r ELISA Kit EZ-Set (DIY Antibody Pairs) (EZ1613)
Protein Target Info & Infographic
Gene/Protein Information For Il23r (Source: Uniprot.org, NCBI)
Gene Name
Il23r
Full Name
Interleukin-23 receptor
Weight
71.722kDa
Superfamily
type I cytokine receptor family
Alternative Names
Interleukin-23 receptor; IL-23 receptor; IL-23R; Il23r; IL23R interleukin 23 receptor interleukin-23 receptor|IL-23 receptor
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Il23r, check out the Il23r Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Il23r: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-IL23 Receptor/Il23r Antibody Picoband® (A00607-1)
Hello CJ!
A00607-1 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
IL-23 Promotes Myocardial I/R Injury by Increasing the Inflammatory Responses and Oxidative Stress Reactions
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Customer Q&As
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4 Customer Q&As for Anti-IL23 Receptor/Il23r Antibody Picoband®
Question
We are currently using anti-IL23 Receptor/Il23r antibody A00607-1 for mouse tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on bovine tissues as well?
T. Jackson
Verified customer
Asked: 2019-12-31
Answer
The anti-IL23 Receptor/Il23r antibody (A00607-1) has not been tested for cross reactivity specifically with bovine tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-12-31
Question
We have been able to see staining in mouse natural killer cell t-cell. Are there any suggestions? Is anti-IL23 Receptor/Il23r antibody supposed to stain natural killer cell t-cell positively?
Verified Customer
Verified customer
Asked: 2019-07-24
Answer
From what I have seen in literature natural killer cell t-cell does express IL23R. From what I have seen in Uniprot.org, IL23R is expressed in secondary oocyte, natural killer cell t-cell, bone marrow, bone marrow skin, among other tissues. Regarding which tissues have IL23R expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 16372191
Bone marrow, and Skin, Pubmed ID: 15489334
Natural killer cell, and T-cell, Pubmed ID: 12023369
Boster Scientific Support
Answered: 2019-07-24
Question
We have tried in the past anti-IL23 Receptor/Il23r antibody for WB on bone marrow in the past. I am using rat, and We want to use the antibody for ELISA next. Our lab want to know about examining bone marrow as well as natural killer cell t-cell in our next experiment. Could give a recommendation on which antibody would work the best for ELISA?
Verified Customer
Verified customer
Asked: 2018-09-10
Answer
I looked at the website and datasheets of our anti-IL23 Receptor/Il23r antibody and it appears that A00607-1 has been validated on rat in both WB and ELISA. Thus A00607-1 should work for your application. Our Boster satisfaction guarantee will cover this product for ELISA in rat even if the specific tissue type has not been validated. We do have a comprehensive range of products for ELISA detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2018-09-10
Question
We were well pleased with the WB result of your anti-IL23 Receptor/Il23r antibody. However we have observed positive staining in natural killer cell t-cell cell membrane using this antibody. Is that expected? Could you tell me where is IL23R supposed to be expressed?
Verified Customer
Verified customer
Asked: 2017-09-12
Answer
From what I have seen in literature, natural killer cell t-cell does express IL23R. Generally IL23R expresses in cell membrane. Regarding which tissues have IL23R expression, here are a few articles citing expression in various tissues:
Bone marrow, Pubmed ID: 16372191
Bone marrow, and Skin, Pubmed ID: 15489334
Natural killer cell, and T-cell, Pubmed ID: 12023369
Boster Scientific Support
Answered: 2017-09-12
