POU2AF1 Antibodies

POU domain class 2-associating factor 1 (POU2AF1)

Transcriptional coactivator that specifically associates with OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2.

It has no intrinsic DNA-binding activity. It admits the POU domains of OCT1 and OCT2.

Gene Information

Human
Gene Name:	POU2AF1
Uniprot:	Q16633
Entrez:	5450

Family

Belongs to:
POU2AF1 family

Alternative Names

B-cell-specific coactivator OBF-1; BOB-1; OBF-1; OBF1BOB1; OCAB; OCA-B; OCT-binding factor 1; POU class 2 associating factor 1; POU domain class 2, associating factor 1; POU domain class 2-associating factor 1; POU domain, class 2, associating factor 1

Molecular Weight

Mass (kDA):
27.436 kDA

Genomic Context

Human
Location:	11q23.1
Sequence:	11; NC_000011.10 (111352251..111379671, complement)

Tissue Specificity

B-cell specific.

Subcellular Localizations

Nucleus.

Diseases

• Lymphoma
• Hodgkin Disease
• B-cell Lymphomas
• Neoplasms
• Classical Hodgkin's Lymphoma
• Diffuse Large B-cell Lymphoma
• Leukemia
• Nodular Lymphocyte Predominant Hodgkin Lymphoma
• Lymphoma, Non-hodgkin
• Malignant Neoplasms
• Chromosomal Translocation
• Plasmacytoma
• Ki-1+ Anaplastic Large Cell Lymphoma

• T-cell Lymphoma
• Multiple Myeloma
• Cell Transformation, Neoplastic
• Autoimmunity
• Burkitt Lymphoma
• Hematologic Neoplasms
• Malignant Paraganglionic Neoplasm

Interacting Proteins

• SIAH1

Pathways

• Cell Development
• Germinal Center Formation
• Cell Differentiation
• B Cell Differentiation
• Immune Response
• Cell Maturation
• Pathogenesis
• Cell Activation
• B Cell Activation
• Immunoglobulin Production
• Localization
• Secretion
• Cell Cycle

• Cell Proliferation
• Methylation
• Lymphocyte Differentiation
• Cell Adhesion
• Mature B Cell Differentiation
• Gene Silencing
• Dna Recombination

PTMs

• Phosphorylation
• Myristoylation

• Deamination
• Deacetylation

• Methylation

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/POU2AF1

Best Uses Of The POU2AF1 Marker

This article provides a brief overview of POU2AF1 recombinant protein. POU2AF1 is up-regulated in airway epithelium differentiation and down-regulated in cigarette smoke. This gene entry marker is available to scientists all over the world. Continue reading to learn more about the uses of POU2AF1 In this article, you will discover some of the most common applications of this protein.

POU2AF1 a recombinant protein from the human POU2AF1 gene

Boster Bio produces the recombinant human protein POU2Af1. Boster Bio makes POU2AF1 that regulates host defense genes and the expression of immunoglobin. It has no intrinsic DNA-binding activity and binds to the POU domain of OCT1. It is essential in germinal centers as well as B-cell responses.

POU2AF1, a regulated gene that is found in B-cells, plays multiple functions in lymphocytes. It regulates genes involved cell survival, adhesion, and cellular metabolism. POU2AF1 also regulates many downstream genes, such as those involved with immune response.

Boster Bio, a biologic company, produces a wide range immunological reagents. These reagents can be used in diagnostic and research. They also make picogram-sensitive ELISA reagents and IHC-optimized, polyclonal antibodies. They offer a wide range of antibodies that can be tailored to your research needs. Boster bio's antibodies can be used in flow applications, WB, IHC and WB. They are quantitatively tested against a panel consisting of 250 cell lines and tissue types to ensure high affinity.

POU2AF1 is a vital component of the BOB.1/OBF.1 genes, which regulates the transition from immature B cell to the periphery. Deficient mice have significantly fewer mature and transitional B cell types, and significantly fewer B and T cells. Pou2af1-deficient mice also lack post-GC-B cells.

It is up-regulated during airway epithelium differentiation

The POU2AF1 genes is expressed in human epithelium, which is a tissue whose genes regulate its differentiation. The gene was detected in the transcribed sequencing readout (RSPCR) data of healthy nonsmokers' airway epithelium. They discovered that POU2AF1 acts as a transcription cofactor to regulate host defense genes. These genes were also upregulated within airway basal/progenitor stem/progenitor tissues. These downstream genes included HLADRA and ID2 genes.

This study used a BCiNS1.1 immortalized human basal cells line to detect POU2AF1 during airway epithelium differentiation. This cell was tested at ALI day 0 and 28. We used secretory andciliated cell markers to verify that the controls were correct. These markers were used as positive controls for differentiation.

The POU2AF1 genes is a novel candidate for identification of subsets in the airway epithelium. This marker is characterized by a unique pattern of expression in different airway epithelium subsets. These data can also be combined with other markers for a better understanding of specific airway epithelium groups.

The complex barrier that is the airway epithelium provides to the lung host defense system functions as a frontline. Two types of host defense molecules are produced by the airway epithelial cells: intracellular antimicrobial proteins and proteins that suppress intracellular disease spreading and surface molecules. These molecules interact directly with Tolllike receptors, which activate specific components in pulmonary host defense. Currently, little is known about how airway epithelium cells regulate these defenses.

It is decreased by smoking cigarettes

Boster Bio researchers are undertaking an innovative study on POU2AF1 in order to find new cancer treatments. The company offers a panel of highly specific, high-affinity primary antibodies that have been cited in the research community for over 25 years. Boster antibodies are validated by Western Blotting, Immunohistochemistry, and ELISA. The results of the study have important implications for the field of cancer research.

It is found in lymphocytes

The POU2AF1 marker, an immunohistochemical probe based in E. coli on the human POU2AF1 gene, is a new immunohistochemical probe. It contains His tag and a 1-256aa DNA sequence domain. It can be stored at -20 to 80degC for long-term storage. Repetitive freeze/thaw cycles should not be allowed. This product is applicable to researchers all over the world.

It is up-regulated by basal cell lines

Based on a protein in E. coli, a POU2AF1 human gene expression assay was developed. This marker is characterized with a His-Tag as well as a 1-256 aa sequence region. It is stable at a temperature range of +2degC to +8degC for up to 1 week, or -20degC to -80degC for long-term storage. It is accessible to all scientists around the world. The POU2AF1 genes is expressed in basal cell lines from a variety o human subjects. Their expression was determined by RTPCR.

The POU2AF1 gene acts as a coactivator for transcription factors and regulates host defense, immunoglobin expression, and host defense. Although it is not directly involved with DNA binding, the POU2AF1 genes acts as a coactivator for transcription factors in B-cell responses. It recognizes OCT1's POU domain and regulates host defense-related genes.

Recent research showed that CSE treatment reduced POU2AF1 expression in ALI-7 and ALI-day 28 samples. We also found that there was no b-tubulin IV stained, which indicated loss of mucociliary differentiation. These data support the use of the POU2AF1 protein as a biomarker in immunofluorescence analysis.

It is decreased by cigarette smoking in the human small airway epithelium

To determine if cigarette smoke can have a detrimental effect upon the gene POU2AF1, first we examined the transcriptional levels for this protein in the human airway epithelium. Human epithelial cells from the airway were grown on ALI. Then, cigarette smoke extract (0 – 6%) was added to the cells. RNA was extracted. Gene expression and protein levels were measured using TaqManPCR.

The researchers then examined transcriptional expression of three genes within human airway epithelial cell cells. The researchers compared the expression levels in cigarette smokers and those in non-smokers. The differences in lung function between the two groups were not significant. Researchers also discovered that the expression of these genes was affected in opposite directions. However, current smokers had lower levels of cathepsin L expression than nonsmokers.

In mice, smoking cigarettes decreased POU2AF1 expression in the airway epithelium. This finding was surprising because POU2AF1 is a host defense gene that regulates inflammation-related microRNAs. POU2AF1 had been thought to be restricted to lymphocytes. The immunohistochemistry staining of human airway epithelial cells showed that this gene is present. Researchers concluded that smoking caused the host defense response of the airway epithelium to be suppressed by down-regulation POU2AF1.

CSE suppressed POU2AF1 in the ALI airway epithelium. CSE led to a decrease of b tubulin IV expression which is indicative for mucociliary differentiate. These results, however, do not prove the causality between smoking and poor airway health. However, it provides further evidence of the mechanism by which CSE causes lung disease.