Anti-BOB1 (POU2AF1) Mouse Monoclonal Antibody [Clone ID: OTI2D3]

Product Name

Anti-BOB1 (POU2AF1) Mouse Monoclonal Antibody [Clone ID: OTI2D3]

View all BOB1 Antibodies

SKU/Catalog Number

M04431-2

Size

100 µl

Description

Boster Bio POU2AF1 mouse monoclonal antibody, clone OTI2D3 (formerly 2D3). Catalog# M04431-2. Tested in FC, IHC, WB. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C as received.

Cite This Product

Anti-BOB1 (POU2AF1) Mouse Monoclonal Antibody [Clone ID: OTI2D3] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04431-2)

Host

Mouse

Contents

PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.

Clonality

Monoclonal

Clone Number

OTI2D3

Isotype

IgG1

Immunogen

Full length human recombinant protein of human POU2AF1 (NP_006226) produced in E.coli.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M04431-2 is reactive to POU2AF1 in Human, Mouse, Rat

Applications

M04431-2 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Validation Images & Assay Conditions

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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY POU2AF1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-POU2AF1 (1:2000).

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Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-POU2AF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min

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Immunohistochemical staining of paraffin-embedded Human tonsil using anti-POU2AF1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.0) at 120°C for 3min

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Flow cytometric Analysis of penetrated Ramos cells

Gene/Protein Information For POU2AF1 (Source: Uniprot.org, NCBI)

Alternative Names

B-cell-specific coactivator OBF-1; BOB-1; OBF-1; OBF1BOB1; OCAB; OCA-B; OCT-binding factor 1; POU class 2 associating factor 1; POU domain class 2, associating factor 1; POU domain class 2-associating factor 1; POU domain, class 2, associating factor 1 POU2AF1 BOB1, OBF-1, OBF1, OCAB POU class 2 homeobox associating factor 1 POU domain class 2-associating factor 1|B-cell-specific coactivator OBF-1|BOB-1|OCA-B|OCT-binding factor 1|POU class 2 associating factor 1

For more info on POU2AF1, check out the POU2AF1 Infographic

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In this infographic, you will see the following information for POU2AF1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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