Click image to see more details
Product Info Summary
| SKU: | PB9528 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IHC, WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-BMPR1B Antibody Picoband®
View all BMPR-IB/ALK-6 Antibodies
SKU/Catalog Number
PB9528
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BMPR1B Antibody Picoband® catalog # PB9528. Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BMPR1B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9528)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human BMPR1B recombinant protein (Position: K14-Q184). Human BMPR1B shares 97.1% amino acid (aa) sequence identity with mouse BMPR1B.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9528 is reactive to BMPR1B in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
57 kDa
Calculated molecular weight
56930 MW
Background of BMPR-IB/ALK-6
BMPR1B (Bone Morphogenetic Protein Receptor Type IB), also known as ALK6, is a protein which in humans is encoded by the BMPR1B gene. BMPR1B is a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. By analysis of a monochromosome hybrid mapping panel and by FISH, Astrom et al. (1999) mapped the BMPR1B gene to chromosome 4q22-q24. Ide et al. (1997) compared BMP receptor expression in normal and cancerous prostate tissues. While BMPR1A and BMPR2 were expressed at similar levels in all prostate tissues, BMPR1B was expressed at a significantly reduced level in cancerous prostate tissue.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9528 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat
Western blot, 0.1-0.5μg/ml, Human
Positive Control
WB: Human Placenta Tissue
IHC: human prostatic cancer tissue
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of BMPR1B using anti-BMPR1B antibody (PB9528).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: Human Placenta Tissue Lysate at 50ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BMPR1B antigen affinity purified polyclonal antibody (Catalog # PB9528) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BMPR1B at approximately 57 kDa. The expected band size for BMPR1B is at 57 kDa.
Click image to see more details
Figure 2. IHC analysis of BMPR1B using anti-BMPR1B antibody (PB9528).
BMPR1B was detected in a paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-BMPR1B Antibody (PB9528) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For BMPR1B (Source: Uniprot.org, NCBI)
Gene Name
BMPR1B
Full Name
Bone morphogenetic protein receptor type-1B
Weight
56930 MW
Superfamily
protein kinase superfamily
Alternative Names
Bone morphogenetic protein receptor type-1B;BMP type-1B receptor;BMPR-1B;2.7.11.30;CDw293;BMPR1B; BMPR1B ALK-6, ALK6, AMDD, BDA1D, BDA2, CDw293 bone morphogenetic protein receptor type 1B bone morphogenetic protein receptor type-1B|BMP type-1B receptor|BMPR-1B|activin receptor-like kinase 6|bone morphogenetic protein receptor, type IB|serine/threonine receptor kinase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BMPR1B, check out the BMPR1B Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BMPR1B: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BMPR1B Antibody Picoband® (PB9528)
Hello CJ!
No publications found for PB9528
*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-BMPR1B Antibody Picoband®?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-BMPR1B Antibody Picoband®
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
1 Customer Q&As for Anti-BMPR1B Antibody Picoband®
Question
We are currently using anti-BMPR1B antibody PB9528 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on bovine tissues as well?
D. Walker
Verified customer
Asked: 2015-08-13
Answer
The anti-BMPR1B antibody (PB9528) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-08-13
