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Product Info Summary
| SKU: | A00730-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Bid Antibody
SKU/Catalog Number
A00730-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Bid Antibody (Catalog # A00730-1). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Bid antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-Bid Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00730-1)
Host
Rabbit
Contents
Bid Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Bid antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human Bid. The immunogen is located within amino acids 120 - 170 of Bid.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
ROCK1 antibody is predicted to not cross-react with other ROCK protein family members.
Reactive Species
A00730-1 is reactive to BID in Human, Mouse
Reconstitution
Observed Molecular Weight
68 kDa
Calculated molecular weight
21995 MW
Background of BID
Apoptosis plays a major role in normal organism development, tissue homeostasis, and removal of damaged cells. Disruption of this process has been implicated in a variety of diseases such as cancer. The Bcl-2 family of proteins is comprised of critical regulators of apoptosis that can be divided into two classes: those that inhibit apoptosis and those that promote cell death. Bid, a pro-apoptotic Bcl-2 family member, is cleaved by caspase-8 in response to apoptotic signals, exposing the Bcl-2 homology 3 (BH3) domain which is normally buried in the full-length protein. The cleaved complex is myris-toylated and translocated to the mitochondrial membrane where it may induce mitochondrial Bax and Bak to oligomerize.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00730-1 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Bid antibody can be used for detection of Bid by Western blot at 0.5 to 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Bid in mouse lung cell lysates with Bid antibody at (A) 0.5
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Immunohistochemical staining of mouse lung tissue using Bid antibody at 2 μg/mL.
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Immunofluorescence of Bid in Mouse Lung cells with Bid antibody at 10 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For BID (Source: Uniprot.org, NCBI)
Gene Name
BID
Full Name
BH3-interacting domain death agonist
Weight
21995 MW
Alternative Names
FP497, BH3-interacting domain death agonist, p22 BID, BID BID FP497 BH3 interacting domain death agonist BH3-interacting domain death agonist|Human BID coding sequence|apoptic death agonist|desmocollin type 4|p22 BID
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BID, check out the BID Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BID: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bid Antibody (A00730-1)
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