Product Info Summary
SKU: | A01263-HRP |
---|---|
Size: | 100μg/vial |
Reactive Species: | Chicken, Human, Monkey, Mouse, Rat, Zebrafish |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-beta-Actin ACTB Antibody (HRP)
View all beta-Actin Antibodies
SKU/Catalog Number
A01263-HRP
Size
100μg/vial
Form
Liquid
Description
Boster Bio Anti-beta-Actin ACTB Antibody (HRP) (Catalog # A01263-HRP). Tested in WB applications. This antibody reacts with Human, Mouse, Rat, Monkey, Chicken, Zebrafish.
Storage & Handling
HRP-Beta-Actin antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-beta-Actin ACTB Antibody (HRP) (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01263-HRP)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4.
Clonality
Polyclonal
Isotype
IgG
Immunogen
HRP-Beta-Actin antibody was raised against a synthetic peptide containing 16 amino acids near the amino terminus of beta-actin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
HRP-Beta-Actin antibody is human, mouse, rat, rabbit, chicken, and drosophila reactive.
Reactive Species
A01263-HRP is reactive to ACTB in Chicken, Human, Monkey, Mouse, Rat, Zebrafish
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
42 kDa
Calculated molecular weight
41737 MW
Background of beta-Actin
Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism. The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging. Beta actin is one of six different actin isoforms which have been identified. Like GAPDH, beta-Actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A01263-HRP is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.02-0.1 μg/ml
Positive Control
WB: human Hela whole cell, human HepG2 whole cell, human MCF-7 whole cell, human K562 whole cell, human A549 whole cell, human A431 whole cell, human U87 whole cell, monkey COS-7 whole cell, rat heart tissue, rat skeletal muscle tissue, rat brain tissue, rat PC-12 whole cell, mouse heart tissue, mouse skeletal tissue, mouse brain tissue, mouse NIH/3T3 whole cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of beta-Actin using anti-beta-Actin antibody (A01263-HRP).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human HepG2 whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human A549 whole cell lysates,
Lane 6: human A431 whole cell lysates,
Lane 7: human U87 whole cell lysates,
Lane 8: monkey COS-7 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-beta-Actin antigen affinity purified polyclonal antibody (Catalog # A01263-HRP) at 0.1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for beta-Actin at approximately 42 kDa. The expected band size for beta-Actin is at 42 kDa.
Click image to see more details
Figure 2. Western blot analysis of beta-Actin using anti-beta-Actin antibody (A01263-HRP).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat heart tissue lysates,
Lane 2: rat skeletal muscle tissue lysates,
Lane 3: rat brain tissue lysates,
Lane 4: rat PC-12 whole cell lysates,
Lane 5: mouse heart tissue lysates,
Lane 6: mouse skeletal tissue lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse NIH/3T3 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-beta-Actin antigen affinity purified polyclonal antibody (Catalog # A01263-HRP) at 0.1 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for beta-Actin at approximately 42 kDa. The expected band size for beta-Actin is at 42 kDa.
Protein Target Info & Infographic
Gene/Protein Information For ACTB (Source: Uniprot.org, NCBI)
Gene Name
ACTB
Full Name
Actin, cytoplasmic 1
Weight
41737 MW
Superfamily
actin family
Alternative Names
HRP-Actin, Beta-Actin, Actin, Beta actin, b actin ACTB BRWS1, PS1TP5BP1 actin beta actin, cytoplasmic 1|I(2)-actin|PS1TP5-binding protein 1|beta cytoskeletal actin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ACTB, check out the ACTB Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ACTB: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-beta-Actin ACTB Antibody (HRP) (A01263-HRP)
Hello CJ!
A01263-HRP has been cited in 90 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Liu ZZ,Han ZD,Liang YK,Chen JX,Wan S,Zhuo YJ,Cai ZD,Deng YL,Lin ZY,Mo RJ,He HC,Zhong WD.TRIB1 induces macrophages to M2 phenotype by inhibiting IKB-zeta in prostate cancer.Cell Signal.2019 Jul;59:152-162.doi:10.1016/j.cellsig.2019.03.017.Epub 2019 Mar 26.PMID:30926388.
Species: Human,Mouse
A01263-HRP usage in article: APP:WB, SAMPLE:PC3 CELL,DU145 CELL AND THP-1 CELL, DILUTION:1:3000
Xu Y,Wang P,Xu C,Shan X,Feng Q.Acrylamide induces HepG2 cell proliferation through upregulation of miR-21 expression.J Biomed Res.2019 Jun 4;33(3):181-191.doi:10.7555/JBR.31.20170016.PMID:28963442;PMCID:PMC6551424.
Species: Human
A01263-HRP usage in article: APP:WB, SAMPLE:HEPG2 CELL, DILUTION:NA
Guo J,Tu J,Hu Y,Song G,Yin Z.Cathepsin G cleaves and activates IL-36γ and promotes the inflammation of psoriasis.Drug Des Devel Ther.2019 Feb 8;13:581-588.doi:10.2147/DDDT.S194765.PMID:30804664;PMCID:PMC6372007.
Species: Human,Mouse
Chen B,Li X,Sun Y,Hou Y,Shen J,Tong C,Ma J.Study of the effects of rabbit scleral fibroblasts on cellular biomechanical properties and MMP-2 expression using two modes of riboflavin/ultraviolet A wave collagen cross-linking.Exp Eye Res.2021 Jul 3:108695.doi:10.1016/j.exer.2021.108695.Epub ahead of print.PMID:34228966.
Species: New Zealand White Rabbit
A01263-HRP usage in article: APP:WB, SAMPLE: FIBROBLASTS, DILUTION:1:100
Cui J,Xu H,Yu J,Li Y,Chen Z,Zou Y,Zhang X,Du Y,Xia J,Wu J.IL-4 inhibits regulatory T cells differentiation by HDAC9-mediated epigenetic regulation.Cell Death Dis.2021 May 18;12(6):501.doi:10.1038/s41419-021-03769-7.PMID:34006836.
Species: Mouse
A01263-HRP usage in article: APP:WB, SAMPLE:T CELL, DILUTION:1:1000
Huang X,Gan H,Tan J,Wang T,Zhao J,Zhao Y.BRCC3 promotes activation of the NLRP6 inflammasome following cerebral ischemia/reperfusion (I/R) injury in rats.Neurosci Lett.2021 May 9;756:135954.doi:10.1016/j.neulet.2021.135954.Epub ahead of print.PMID:33979701.
Species: Rat
A01263-HRP usage in article: APP:WB, SAMPLE:CEREBERAL CORTEX, DILUTION:1:2000
He G,Gu J,Wang H,Cheng S,Xiong Q,Ke M,Hu Y,Feng J,Song L,Liu Z,Xu Y.Nr2e1 deficiency aggravates insulin resistance and chronic inflammation of visceral adipose tissues in a diet-induced obese mice model.Life Sci.2021 Apr 26;278:119562.doi:10.1016/j.lfs.2021.119562.Epub ahead of print.PMID:33915130.
Species: Mouse
A01263-HRP usage in article: APP: WB, SAMPLE:ADIPOSE TISSUE, DILUTION:1:1000
Huaqing Zheng,Xiaolong Li,Xin Zeng,Chengcui Huang,Mingming Ma,Xiaofei Lv,Yajuan Zhang,Lu Sun,Guanlei Wang,Yanhua Du,Yongyuan Guan, TMEM16A inhibits angiotensin II-induced basilar artery smooth muscle cell migration in a WNK1-dependent manner,Acta Pharmaceutica Sinica B,2021,,ISSN 2211-3835,https://doi.org/10.1016/j.apsb.2021.04. 013.
Species: Mouse
A01263-HRP usage in article: APP:WB, SAMPLE:BASMCS, DILUTION:NA
Hu J,Cai D,Zhao Z,Zhong G-C and Gong J (2021) Suppression of Heterogeneous Nuclear Ribonucleoprotein C Inhibit Hepatocellular Carcinoma Proliferation, Migration, and Invasion via Ras/MAPK Signaling Pathway.Front.Oncol.11:659676.doi:10.3389/fonc.2021.659676
Species: Human,Mouse
A01263-HRP usage in article: APP:WB, SAMPLE:HCC TISSUE, DILUTION:1:2000
Lamptey J,Li F,Adu-Gyamfi EA,Chen XM,Czika A,Otoo A,Liu TH,Wang YX,Ding YB.Downregulation of fascin in the first trimester placental villi is associated with early recurrent miscarriage.Exp Cell Res.2021 Apr 16;403(1):112597.doi:10.1016/j.yexcr.2021.112597.Epub ahead of print.PMID:33862100.
Species: Human
A01263-HRP usage in article: APP: WB, SAMPLE:PLACENTAL TISSUE, DILUTION: 1:1000
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