Product Info Summary
SKU: | M00094-3 |
---|---|
Size: | 50 µl |
Reactive Species: | Human |
Host: | Rabbit |
Application: | IF, WB |
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Product info
Product Name
Anti-Transferrin Antibody
View all Transferrin Antibodies
SKU/Catalog Number
M00094-3
Size
50 µl
Description
Boster Bio Anti-Transferrin Antibody (Catalog # M00094-3). Tested in WB, IF application(s). This antibody reacts with Human.
Storage & Handling
Maintain refrigerated at 2-8°C for up to 2 weeks. For long-term storage, store at -20°C in small aliquots to prevent freeze-thaw cycles.
Cite This Product
Anti-Transferrin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00094-3)
Host
Rabbit
Contents
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
This Transferrin antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 101-135 amino acids from human Transferrin.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00094-3 is reactive to TF in Human
Reconstitution
Calculated molecular weight
77050 Da
Background of Transferrin
Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00094-3 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF: 1:25
WB: 1:2000
Validation Images & Assay Conditions
Click image to see more details
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells labeling Transferrin with Cleaved PARP (Asp214) Antibody at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-rabbit IgG secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HepG2 cell line. The nuclear counter stain is DAPI (blue).
Click image to see more details
All lanes : Anti-Transferrin Antibody at 1:2000 dilution
Lane 1: human plasma lysate
Lane 2: human fetal liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 77kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Click image to see more details
All lanes : Anti-Transferrin Antibody at 1:2000 dilution
Lane 1: human plasma lysate
Lane 2: human liver lysate
Lane 3: human fetal liver lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 77 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.
Protein Target Info & Infographic
Gene/Protein Information For TF (Source: Uniprot.org, NCBI)
Gene Name
TF
Full Name
Serotransferrin
Weight
77050 Da
Superfamily
transferrin family
Alternative Names
Serotransferrin, Transferrin, Beta-1 metal-binding globulin, Siderophilin, TF TF HEL-S-71p, PRO1557, PRO2086QTL1, TF transferrin serotransferrin|beta-1 metal-binding globulin|epididymis secretory sperm binding protein Li 71p|siderophilin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TF, check out the TF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transferrin Antibody (M00094-3)
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