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Product Info Summary
| SKU: | M00094-1 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Pig, Rabbit |
| Host: | Mouse |
| Application: | ELISA, IHC-P, ICC, WB, Functional Assay, RIA |
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Product info
Product Name
Anti-Transferrin Purified Azide Free TF Monoclonal Antibody
View all Transferrin Antibodies
SKU/Catalog Number
M00094-1
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Transferrin Purified Azide Free TF Monoclonal Antibody (Catalog# M00094-1). Tested in WB, IHC-P, ICC, ELISA, RIA, Functional Assay application(s). This antibody reacts with Human, Pig, Rabbit.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-Transferrin Purified Azide Free TF Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00094-1)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, azide-free
Clonality
Monoclonal
Clone Number
HTF-14
Isotype
Mouse IgG1
Immunogen
Purified porcine transferrin. The antibody HTF-14 recognizes an epitope located in the N-terminal domain of human serum transferrin, a 77 kDa single polypeptide chain glycoprotein (member of the iron binding family of proteins). It is synthesised in the liver and consists of two domains each having a high affinity reversible binding site for Fe3+.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00094-1 is reactive to TF in Human, Pig, Rabbit
Reconstitution
Observed Molecular Weight
42 kDa
Calculated molecular weight
77064 MW
Background of Transferrin
Transferrin is a monomeric glycoprotein of approximately 77 kDa, which serves as an iron-transporter. In normal plasma, transferrin has a concentration of 25-50 µmol / liter, and is usually about one-third saturated with iron, thus providing a large buffering capacity in case of an acute increase in plasma iron levels. Cells take up transferrin-iron complexes (holotransferrin) using transferrin receptor dimers. Upon binding of holotransferrin, the receptor is internalized by clathrin-mediated endocytosis. Acidification of endosomes by vesicular membrane proton pumps leads to dissociation of iron ions, whereas transferrin (apotransferrin) remains associated with its receptor (CD71) and recycles to the cell surface, where apotransferrin is released upon exposure to normal pH. Internalization of labeled transferrin thus represents an usefull approach to study endocytosis. Serum concentration rises in iron deficiency and pregnancy and falls in iron overload, infection and inflammatory conditions. Iron/transferrin complex is essential in haemoglobin synthesis and for certain types of cell division.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00094-1 is guaranteed for ELISA, IHC-P, ICC, WB, Functional Assay, RIA Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Functional application: The antibody HTF-14 blocks binding of transferrin to its receptor.
Immunohistochemistry (paraffin sections): 10 μg/ml; positive tissue: placenta.
Western blotting: non-reducing conditions, recommended dilution: 1-2 μg/ml.
Validation Images & Assay Conditions
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Figure 1. Western blotting validation for Anti-Transferrin Purified Azide Free TF Monoclonal Antibody M00094-1
Human transferrin detected by the mouse monoclonal antibody HTF-14.1. hTransferrin; 5 µg/well (red. con.)
2. hTransferrin; 3 µg/well (red. con.)
3. hTransferrin; 1 µg/well (red. con.)
M Low Range marker (Bio-Rad)
4. hTransferrin; 1 µg/well (non-red. con.)
5. hTransferrin; 3 µg/well (non-red. con.)
6. hTransferrin; 5 µg/well (non-red. con.)
Electrophoresis was performed on a SDS-PAGE gel. To determine SDS-PAGE gel concentration
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Figure 2. Immunohistochemistry validation of TF using Anti-Transferrin Purified Azide Free TF Monoclonal Antibody (M00094-1).
Immunohistochemistry staining of human placenta (paraffin sections) using anti-transferrin (HTF-14).Commercially tested by LifeSpan BioSciences.
For more protocol information of IHC
Protein Target Info & Infographic
Gene/Protein Information For TF (Source: Uniprot.org, NCBI)
Gene Name
TF
Full Name
Serotransferrin
Weight
77064 MW
Superfamily
transferrin family
Alternative Names
TFQTL1, PRO1557, PRO2086 TF HEL-S-71p, PRO1557, PRO2086QTL1, TF transferrin serotransferrin|beta-1 metal-binding globulin|epididymis secretory sperm binding protein Li 71p|siderophilin
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on TF, check out the TF Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for TF: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Transferrin Purified Azide Free TF Monoclonal Antibody (M00094-1)
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