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Product Info Summary
| SKU: | A12290 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC-P, WB |
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Product info
Product Name
Anti-Beclin 2 BECN2 Antibody
SKU/Catalog Number
A12290
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Beclin 2 BECN2 Antibody (Catalog # A12290). Tested in ELISA, WB, IHC-P, IF applications. This antibody reacts with Human.
Storage & Handling
Beclin 2 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Cite This Product
Anti-Beclin 2 BECN2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A12290)
Host
Rabbit
Contents
Beclin 2 antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Beclin 2 antibody was raised against a 16 amino acid peptide near the amino terminus of human Beclin 2. The immunogen is located within amino acids 30 - 80 of Beclin 2.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Beclin 2 antibody is human specific. At least two isoforms of Beclin 2 are known to exist; this antibody will only detect the longer isoform. Beclin 2 antibody is predicted to not cross-react with Beclin 1.
Reactive Species
A12290 is reactive to BECN2 in Human
Applications
A12290 is guaranteed for ELISA, IF, IHC-P, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
48153 MW
Background of Beclin 2
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells (1,2). Beclin 2 is a mammalian specific homolog of the autophagy protein Beclin 1 (3). Like Beclin 1, Beclin 2 interacts with Bcl-2 and class III PI3K complex components. However, Beclin 2 functions in an additional lysosomal degradation pathway and is required for ligand-induced endolysosomal degradation of several G protein-coupled receptors (3). Beclin 2 is also required for agonist-induced lysosome-mediated degradation of EGFR in lung cancer cells, suggesting that it may also play a role in regulating other intracellular signaling pathways (4).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Beclin 2 antibody can be used for detection of Beclin 2 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Beclin 2 in Jurkat cell lysate with Beclin 2 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.
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Immunohistochemistry of Beclin 2 in human lung carcinoma tissue with Beclin 2 antibody at 5 μg/ml.
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Immunofluorescence of Beclin 2 in human lung carcinoma tissue with Beclin 2 antibody at 20 μg/ml.
Protein Target Info & Infographic
Gene/Protein Information For BECN2 (Source: Uniprot.org, NCBI)
Gene Name
BECN2
Full Name
Beclin-2
Weight
48153 MW
Superfamily
beclin family
Alternative Names
BECN1 L1; BECN1L1; BECN1P1 beclin 1, autophagy related, pseudogene 1; BECN1P1 BECN2 BECN1L1, BECN1P1 beclin 2 beclin-2|Beclin-1-like protein 1|beclin-1 autophagy-related pseudogene 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BECN2, check out the BECN2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BECN2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Beclin 2 BECN2 Antibody (A12290)
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