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Product Info Summary
| SKU: | M04965 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC |
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Product info
Product Name
Anti-Laminin Antibody
SKU/Catalog Number
M04965
Size
100ul
Description
Boster Bio Anti-Laminin Antibody catalog # M04965. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Laminin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04965)
Host
Rabbit
Contents
Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN3
Clonality
Polyclonal
Immunogen
Laminin isolated from mouse EHS cells
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M04965 is reactive to LAMA1 in Human, Mouse, Rat
Reconstitution
Calculated molecular weight
337084 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M04965 is guaranteed for IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1,000-5,000. IF/ICC and IHC: 1:1,000-5,000
Validation Images & Assay Conditions
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Western blot analysis of different tissue lysates using rabbit pAb to laminin, M04965, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, and [4] cow spinal cord lysate. The strong band above the 280kDa mark corresponds to full length laminin proteins. Smaller proteolytic fragments of laminin are also detected with this antibody.
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Immunohistological analysis of brain stem section stained with rabbit pAb to laminin, M04965, dilution 1:1,000 in red, and costained with chicken pAb to Myelin Basic Protein (MBP), dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45M, and free-floating sections were stained with the above antibodies. The laminin antibody is an excellent marker of basement membranes surrounding blood vessels, while the MBP antibody stains the myelin sheathes around axons.
Protein Target Info & Infographic
Gene/Protein Information For LAMA1 (Source: Uniprot.org, NCBI)
Gene Name
LAMA1
Full Name
Laminin subunit alpha-1
Weight
337084 MW
Alternative Names
Laminin subunit alpha-1;Laminin A chain;Laminin-1 subunit alpha;Laminin-3 subunit alpha;S-laminin subunit alpha;S-LAM alpha;LAMA1;LAMA; Lama1|AA408497, L, Lama|laminin, alpha 1|laminin subunit alpha-1|S-LAM alpha|S-laminin subunit alpha|laminin A chain|laminin-1 subunit alpha|laminin-3 subunit alpha
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on LAMA1, check out the LAMA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for LAMA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Laminin Antibody (M04965)
Hello CJ!
M04965 has been cited in 7 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Early protective effect of ischemic preconditioning on small intestinal graft in rats
Decellularized scaffold of cryopreserved rat kidney retains its recellularization potential
Effects of fasudil on the cytoskeleton of trabecular meshwork cells and outflow facility in bovine eyes
Autocrine fibronectin from differentiating mesenchymal stem cells induces the neurite elongation in vitro and promotes nerve fiber regeneration in transected spinal cord injury
Zhu B, Xu T, Yuan J, Guo X, Liu D. Plos One. 2013 Sep 19;8(9):E76282. Doi: 10.1371/Journal.Pone.0076282. Ecollection 2013. Transcriptome Sequencing Reveals Differences Between Primary And Secondary Hair Follicle-Derived Dermal Papilla Cells Of The...
Chen Tl, Wang Hl, Liu Yh, Fang Y, Tan Rz, Zhou Ph, Zhou Q, Lv Xy. Scientificworldjournal. 2014;2014:682189. Doi: 10.1155/2014/682189. Epub 2014 Jul 14. Highly Effective Ex Vivo Gene Manipulation To Study Kidney Development Using Self-Complementary...
Zhang Rp, Liu Hh, Li Qq, Wang Y, Liu Jy, Hu Jw, Yan Xp, Gou H, Li L, Wang Jw. Poult Sci. 2014 Dec;93(12):3104-11. Doi: 10.3382/Ps.2014-04145. Epub 2014 Oct 11. Gene Expression Patterns, And Protein Metabolic And Histological Analyses For Muscle De...
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Customer Q&As
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3 Customer Q&As for Anti-Laminin Antibody
Question
We have been able to see staining in rat metanephros. Do you have any suggestions? Is anti-Laminin antibody supposed to stain metanephros positively?
Verified Customer
Verified customer
Asked: 2019-08-27
Answer
From literature metanephros does express LAMA1. From Uniprot.org, LAMA1 is expressed in metanephros, liver, among other tissues. Regarding which tissues have LAMA1 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2019-08-27
Question
Our lab were well pleased with the WB result of your anti-Laminin antibody. However we have observed positive staining in liver extracellular space using this antibody. Is that expected? Could you tell me where is LAMA1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-05-09
Answer
From what I have seen in literature, liver does express LAMA1. Generally LAMA1 expresses in secreted, extracellular space, extracellular. Regarding which tissues have LAMA1 expression, here are a few articles citing expression in various tissues:
Liver, Pubmed ID: 19159218
Boster Scientific Support
Answered: 2019-05-09
Question
We are currently using anti-Laminin antibody M04965 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2018-10-09
Answer
The anti-Laminin antibody (M04965) has not been validated for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-10-09
