Anti-Laminin Antibody

Product Name

Anti-Laminin Antibody

View all Lama1 Antibodies

SKU/Catalog Number

M04965

Size

100ul

Description

Boster Bio Anti-Laminin Antibody catalog # M04965. Tested in IHC applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Laminin Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M04965)

Host

Rabbit

Contents

Purified antibody at 1mg/mL in 50% PBS, 50% glycerol plus 5mM NaN₃

Clonality

Polyclonal

Immunogen

Laminin isolated from mouse EHS cells

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

M04965 is reactive to Lama1 in Human, Mouse, Rat

Applications

M04965 is guaranteed for IHC Boster Guarantee

Observed Molecular Weight

Calculated molecular weight

337084 MW

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

WB 1:1,000-5,000. IF/ICC and IHC: 1:1,000-5,000

Validation Images & Assay Conditions

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Western blot analysis of different tissue lysates using rabbit pAb to laminin, M04965, dilution 1:5,000 in green: [1] protein standard (red), [2] rat brain, [3] rat spinal cord, and [4] cow spinal cord lysate. The strong band above the 280kDa mark corresponds to full length laminin proteins. Smaller proteolytic fragments of laminin are also detected with this antibody.

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Immunohistological analysis of brain stem section stained with rabbit pAb to laminin, M04965, dilution 1:1,000 in red, and costained with chicken pAb to Myelin Basic Protein (MBP), dilution 1:5,000 in green. The blue is DAPI staining of nuclear DNA. Following transcardial perfusion of rat with 4% paraformaldehyde, brain was post fixed for 24 hours, cut to 45M, and free-floating sections were stained with the above antibodies. The laminin antibody is an excellent marker of basement membranes surrounding blood vessels, while the MBP antibody stains the myelin sheathes around axons.

Gene/Protein Information For Lama1 (Source: Uniprot.org, NCBI)

Alternative Names

LAMA; Laminin A chain; laminin subunit alpha-1; laminin, alpha 1; Laminin-1 subunit alpha; Laminin-3 subunit alpha; S-LAM alpha; S-laminin subunit alpha Lama1|AA408497, L, Lama|laminin, alpha 1|laminin subunit alpha-1|S-LAM alpha|S-laminin subunit alpha|laminin A chain|laminin-1 subunit alpha|laminin-3 subunit alpha

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