Click image to see more details
Product Info Summary
| SKU: | P00327 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | WB |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-Phospho-Beclin-1 (Ser234) BECN1 Antibody
SKU/Catalog Number
P00327
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-Phospho-Beclin-1 (Ser234) BECN1 Antibody (Catalog # P00327). Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C. After date of receipt, stable for at least 1 year at -20°C.
Cite This Product
Anti-Phospho-Beclin-1 (Ser234) BECN1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # P00327)
Host
Rabbit
Contents
10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Standard Protein
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser234 of human beclin-1, conjugated to keyhole limpet hemocyanin (KLH). Immunogen species is Human.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
P00327 is reactive to BECN1 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
52 kDa
Calculated molecular weight
51896 MW
Background of Beclin 1
Beclin-1 plays a key role in tumor suppression and autophagy. It has been suggested that autophagy proteins such as beclin-1 can inhibit tumorigenesis and that decreased expression of these proteins may contribute to the development of human malignancies (Liang et al., 1999). Beclin-1 has been shown to interact with 14-3-3 proteins through Akt-mediated phosphorylation at Ser-234 (Wang et al., 2012).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
P00327 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:250
Validation Images & Assay Conditions
Click image to see more details
Western blot of wild type (WT) beclin-1 expressed in HeLa cell lysate showing specific immunolabeling of beclin-1 phosphorylated at Ser234. This immunolabeling is decreased in the beclin-1 double alanine substitution mutant (S234A, S295A: Mut) expressed in HeLa cell lysate.
Protein Target Info & Infographic
Gene/Protein Information For BECN1 (Source: Uniprot.org, NCBI)
Gene Name
BECN1
Full Name
Beclin-1
Weight
51896 MW
Superfamily
beclin family
Alternative Names
ATG6 autophagy related 6 homolog; ATG6; beclin 1 (coiled-coil, moesin-like BCL2 interacting protein); beclin 1 (coiled-coil, moesin-like BCL2-interacting protein); Beclin 1; beclin 1, autophagy related; beclin1; beclin-1; BECN1; Coiled-coil myosin-like BCL2-interacting protein; GT197; Protein GT197; VPS30 BECN1 ATG6, VPS30, beclin1 beclin 1 beclin-1|ATG6 autophagy related 6 homolog|beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)|beclin 1, autophagy related|coiled-coil myosin-like BCL2-interacting protein|testis secretory sperm-binding protein Li 215e
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BECN1, check out the BECN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BECN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Phospho-Beclin-1 (Ser234) BECN1 Antibody (P00327)
Hello CJ!
P00327 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Song L,Yao L,Zhang L,Piao Z,Lu Y.Schizandrol A protects against Aβ1-42-induced autophagy via activation of PI3K/AKT/mTOR pathway in SH-SY5Y cells and primary hippocampal neurons.Naunyn Schmiedebergs Arch Pharmacol.2020 Sep;393(9):1739-1752.doi:10.1007/s00
Species: Human
P00327 usage in article: APP:WB, SAMPLE:SH-SY5Y CELLS, DILUTION:1:400
Recombinant Newcastle disease virus (rL-RVG) triggers autophagy and apoptosis in gastric carcinoma cells by inducing ER stress
Song J, Hu Y, Li J, Zheng H, Wang J, Guo L, Shi H, Liu L. Arch Virol. 2018 Jan;163(1):135-144. doi: 10.1007/s00705-017-3592-x. Epub 2017 Oct 19. Suppression of the toll-like receptor 7-dependent type I interferon production pathway by autophagy re...
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-Phospho-Beclin-1 (Ser234) BECN1 Antibody?
Submit a review and receive an Amazon gift card.
- $30 for a review with an image
0 Reviews For Anti-Phospho-Beclin-1 (Ser234) BECN1 Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
15 Customer Q&As for Anti-Phospho-Beclin-1 (Ser234) BECN1 Antibody
Question
Is this P00327 anti-Phospho-Beclin-1 (Ser234) antibody reactive to the isotypes of BECN1?
Verified Customer
Verified customer
Asked: 2020-01-22
Answer
The immunogen of P00327 anti-Phospho-Beclin-1 (Ser234) antibody is The antigen is a phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser234 of human beclin-1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-22
Question
Is there a BSA free version of anti-Phospho-Beclin-1 (Ser234) antibody P00327 available?
Verified Customer
Verified customer
Asked: 2019-12-17
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-Phospho-Beclin-1 (Ser234) antibody P00327 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-12-17
Question
We are currently using anti-Phospho-Beclin-1 (Ser234) antibody P00327 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
The anti-Phospho-Beclin-1 (Ser234) antibody (P00327) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-08-15
Question
My boss were satisfied with the WB result of your anti-Phospho-Beclin-1 (Ser234) antibody. However we have observed positive staining in brain cytoplasm using this antibody. Is that expected? Could you tell me where is BECN1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2019-08-09
Answer
From what I have seen in literature, brain does express BECN1. Generally BECN1 expresses in cytoplasm. Regarding which tissues have BECN1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9765397, 10395800
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 14702039
Mammary gland, Pubmed ID: 7490091
Boster Scientific Support
Answered: 2019-08-09
Question
Is a blocking peptide available for product anti-Phospho-Beclin-1 (Ser234) antibody (P00327)?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
We do provide the blocking peptide for product anti-Phospho-Beclin-1 (Ser234) antibody (P00327). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-05-21
Question
I see that the anti-Phospho-Beclin-1 (Ser234) antibody P00327 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-02
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-02
Question
Will P00327 anti-Phospho-Beclin-1 (Ser234) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
C. Jackson
Verified customer
Asked: 2018-11-14
Answer
You can see on the product datasheet, P00327 anti-Phospho-Beclin-1 (Ser234) antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-11-14
Question
I am interested in to test anti-Phospho-Beclin-1 (Ser234) antibody P00327 on human erythroleukemia for research purposes, then I may be interested in using anti-Phospho-Beclin-1 (Ser234) antibody P00327 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-09-17
Answer
The products we sell, including anti-Phospho-Beclin-1 (Ser234) antibody P00327, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-09-17
Question
We have been able to see staining in human mammary gland. What should we do? Is anti-Phospho-Beclin-1 (Ser234) antibody supposed to stain mammary gland positively?
Verified Customer
Verified customer
Asked: 2018-09-06
Answer
From what I have seen in literature mammary gland does express BECN1. From what I have seen in Uniprot.org, BECN1 is expressed in frontal cortex, brain, liver, cervix, mammary gland, cervix carcinoma, erythroleukemia, among other tissues. Regarding which tissues have BECN1 expression, here are a few articles citing expression in various tissues:
Brain, Pubmed ID: 9765397, 10395800
Cervix, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 18691976
Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 14702039
Mammary gland, Pubmed ID: 7490091
Boster Scientific Support
Answered: 2018-09-06
Question
I would like using your anti-Phospho-Beclin-1 (Ser234) antibody for cellular response to glucose starvation studies. Has this antibody been tested with western blotting on hela cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2018-05-29
Answer
We appreciate your inquiry. This P00327 anti-Phospho-Beclin-1 (Ser234) antibody is validated on hela cell lysate. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-05-29
Question
Does anti-Phospho-Beclin-1 (Ser234) antibody P00327 work for WB with erythroleukemia?
Verified Customer
Verified customer
Asked: 2017-05-23
Answer
According to the expression profile of erythroleukemia, BECN1 is highly expressed in erythroleukemia. So, it is likely that anti-Phospho-Beclin-1 (Ser234) antibody P00327 will work for WB with erythroleukemia.
Boster Scientific Support
Answered: 2017-05-23
Question
I was wanting to use your anti-Phospho-Beclin-1 (Ser234) antibody for WB for human erythroleukemia on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human erythroleukemia identification?
S. Rodriguez
Verified customer
Asked: 2015-10-12
Answer
As indicated on the product datasheet, P00327 anti-Phospho-Beclin-1 (Ser234) antibody has been validated for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human erythroleukemia in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-10-12
Question
Here is the WB image, lot number and protocol we used for erythroleukemia using anti-Phospho-Beclin-1 (Ser234) antibody P00327. Please let me know if you require anything else.
K. Johnson
Verified customer
Asked: 2015-09-30
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2015-09-30
Question
I have a question about product P00327, anti-Phospho-Beclin-1 (Ser234) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Krishna
Verified customer
Asked: 2013-11-29
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free P00327 anti-Phospho-Beclin-1 (Ser234) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-11-29
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for erythroleukemia using anti-Phospho-Beclin-1 (Ser234) antibody P00327. Let me know if you need anything else.
W. Yang
Verified customer
Asked: 2013-08-01
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-08-01
