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Product Info Summary
| SKU: | M00327-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Dog, Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-Beclin 1 (BECN1) Mouse Monoclonal Antibody [Clone ID: OTI1F1]
SKU/Catalog Number
M00327-1
Size
100 µl
Description
Boster Bio BECN1 (Beclin 1) mouse monoclonal antibody, clone OTI1F1 (formerly 1F1). Catalog# M00327-1. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat, Dog.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Beclin 1 (BECN1) Mouse Monoclonal Antibody [Clone ID: OTI1F1] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00327-1)
Host
Mouse
Contents
PBS (PH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
OTI1F1
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human BECN1 (NP_003757) produced in HEK293T cell.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00327-1 is reactive to BECN1 in Dog, Human, Mouse, Rat
Reconstitution
Calculated molecular weight
51.896kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00327-1 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BECN1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BECN1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BECN1 monoclonal antibody.
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Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-BECN1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either BECN1 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-BECN1 antibody (M00327-1)
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Flow cytometric Analysis of Hela cells
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Flow cytometric Analysis of Jurkat cells
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Anti-BECN1 mouse monoclonal antibody (M00327-1) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY BECN1.
Protein Target Info & Infographic
Gene/Protein Information For BECN1 (Source: Uniprot.org, NCBI)
Gene Name
BECN1
Full Name
Beclin-1
Weight
51.896kDa
Superfamily
beclin family
Alternative Names
ATG6 autophagy related 6 homolog; ATG6; beclin 1 (coiled-coil, moesin-like BCL2 interacting protein); beclin 1 (coiled-coil, moesin-like BCL2-interacting protein); Beclin 1; beclin 1, autophagy related; beclin1; beclin-1; BECN1; Coiled-coil myosin-like BCL2-interacting protein; GT197; Protein GT197; VPS30 BECN1 ATG6, VPS30, beclin1 beclin 1 beclin-1|ATG6 autophagy related 6 homolog|beclin 1 (coiled-coil, moesin-like BCL2-interacting protein)|beclin 1, autophagy related|coiled-coil myosin-like BCL2-interacting protein|testis secretory sperm-binding protein Li 215e
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BECN1, check out the BECN1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BECN1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Beclin 1 (BECN1) Mouse Monoclonal Antibody [Clone ID: OTI1F1] (M00327-1)
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No publications found for M00327-1
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