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Product Info Summary
| SKU: | M00181-1 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | Flow Cytometry, IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-Bcl-XL BCL2L1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00181-1
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-Bcl-XL BCL2L1 Rabbit Monoclonal Antibody catalog # M00181-1. Tested in WB, IHC, ICC/IF, IP, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Bcl-XL BCL2L1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00181-1)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
AOD-2
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human Bcl-XL
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M00181-1 is reactive to BCL2L1 in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
30 kDa
Calculated molecular weight
26049 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00181-1 is guaranteed for Flow Cytometry, IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
IP 1:50
FC 1:50
Positive Control
WB: human Hela whole cell, human K562 whole cell, human 293T whole cell, human SH-SY5Y whole cell, rat spleen tissue, rat C6 whole cell, mouse spleen tissue, mouse Neuro-2a whole cell
IHC: Human pituitary tumor tissue, Human liver cancer tissue, human liver cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of BCL2L1 using anti-BCL2L1 antibody (M00181-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human K562 whole cell lysates,
Lane 3: human 293T whole cell lysates,
Lane 4: human SH-SY5Y whole cell lysates,
Lane 5: rat spleen tissue lysates,
Lane 6: rat C6 whole cell lysates,
Lane 7: mouse spleen tissue lysates,
Lane 8: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BCL2L1 antigen affinity purified monoclonal antibody (Catalog # M00181-1) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BCL2L1 at approximately 30 kDa. The expected band size for BCL2L1 is at 26 kDa.
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Immunohistochemical analysis of paraffin-embedded Human liver cancer, using the Antibody at 1:400 dilution.
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Immunohistochemical analysis of paraffin-embedded Human pituitary tumor, using the Antibody at 1:400 dilution.
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Immunohistochemical analysis of paraffin-embedded human liver cancer, using Bcl-XL Antibody.
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Immunofluorescent analysis using the Antibody at 1:50 dilution.
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Immunofluorescent analysis of Hela cells, using Bcl-XL Antibody .
Protein Target Info & Infographic
Gene/Protein Information For BCL2L1 (Source: Uniprot.org, NCBI)
Gene Name
BCL2L1
Full Name
Bcl-2-like protein 1
Weight
26049 MW
Superfamily
Bcl-2 family
Alternative Names
Bcl-2-like protein 1;Bcl2-L-1;Apoptosis regulator Bcl-X;BCL2L1;BCL2L, BCLX; BCL2L1 BCL-XL/S, BCL2L, BCLX, Bcl-X, PPP1R52 BCL2 like 1 bcl-2-like protein 1|apoptosis regulator Bcl-X|protein phosphatase 1, regulatory subunit 52
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BCL2L1, check out the BCL2L1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BCL2L1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Bcl-XL BCL2L1 Rabbit Monoclonal Antibody (M00181-1)
Hello CJ!
M00181-1 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Chen H,Sheng H,Zhao Y,Zhu G.Piperine Inhibits Cell Proliferation and Induces Apoptosis of Human Gastric Cancer Cells by Downregulating Phosphatidylinositol 3-Kinase (PI3K)/Akt Pathway.Med Sci Monit.2020 Dec 31;26:e928403.doi:10.12659/MSM.928403.PMID:33382
Species: Human,Mouse
M00181-1 usage in article: APP:WB, SAMPLE:SNU-16 CELL, DILUTION:NA
Relationship between Egr-1 gene expression and apoptosis in esophageal carcinoma and precancerous lesions
Zhou X, Zhang Y, Li Y, Hao X, Liu X, Wang Y. Cancers (Basel). 2012 Dec 4;4(4):1318-32. Doi: 10.3390/Cancers4041318. Azithromycin Synergistically Enhances Anti-Proliferative Activity Of Vincristine In Cervical And Gastric Cancer Cells.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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16 Customer Q&As for Anti-Bcl-XL BCL2L1 Rabbit Monoclonal Antibody
Question
I see that the anti-Bcl-XL Rabbit Monoclonal antibody M00181-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-02-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-02-12
Question
Is this M00181-1 anti-Bcl-XL Rabbit Monoclonal antibody reactive to the isotypes of BCL2L1?
Verified Customer
Verified customer
Asked: 2020-01-17
Answer
The immunogen of M00181-1 anti-Bcl-XL Rabbit Monoclonal antibody is A synthesized peptide derived from human Bcl-XL. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2020-01-17
Question
Can you help my question with product M00181-1, anti-Bcl-XL Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-12-12
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00181-1 anti-Bcl-XL Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-12-12
Question
Is there a BSA free version of anti-Bcl-XL Rabbit Monoclonal antibody M00181-1 available?
Verified Customer
Verified customer
Asked: 2019-08-28
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Bcl-XL Rabbit Monoclonal antibody M00181-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-08-28
Question
Does M00181-1 anti-Bcl-XL Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
H. Bhatt
Verified customer
Asked: 2019-07-02
Answer
It shows on the product datasheet, M00181-1 anti-Bcl-XL Rabbit Monoclonal antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-02
Question
you antibody to test anti-Bcl-XL Rabbit Monoclonal antibody M00181-1 on rat lung for research purposes, then I may be interested in using anti-Bcl-XL Rabbit Monoclonal antibody M00181-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-06-18
Answer
The products we sell, including anti-Bcl-XL Rabbit Monoclonal antibody M00181-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-06-18
Question
Here is the WB image, lot number and protocol we used for lung using anti-Bcl-XL Rabbit Monoclonal antibody M00181-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-06-17
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-06-17
Question
Our lab used your anti-Bcl-XL Rabbit Monoclonal antibody for IF on lung in a previous project. I am using human, and I plan to use the antibody for IHC next. Our lab want to know about examining lung as well as upper lobe of left lung in our next experiment. Could you please give me some suggestion on which antibody would work the best for IHC?
F. Zhao
Verified customer
Asked: 2019-02-05
Answer
I viewed the website and datasheets of our anti-Bcl-XL Rabbit Monoclonal antibody and I see that M00181-1 has been validated on human in both IF and IHC. Thus M00181-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IHC in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IHC detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-02-05
Question
We are currently using anti-Bcl-XL Rabbit Monoclonal antibody M00181-1 for mouse tissue, and we are content with the IP results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2019-01-28
Answer
The anti-Bcl-XL Rabbit Monoclonal antibody (M00181-1) has not been tested for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-28
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-Bcl-XL Rabbit Monoclonal antibody M00181-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2018-12-20
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-12-20
Question
We have been able to see staining in mouse colon carcinoma. Any tips? Is anti-Bcl-XL Rabbit Monoclonal antibody supposed to stain colon carcinoma positively?
Verified Customer
Verified customer
Asked: 2018-12-04
Answer
From what I have seen in literature colon carcinoma does express BCL2L1. From what I have seen in Uniprot.org, BCL2L1 is expressed in upper lobe of left lung, colon carcinoma, lung, among other tissues. Regarding which tissues have BCL2L1 expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 17974005
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2018-12-04
Question
Is a blocking peptide available for product anti-Bcl-XL Rabbit Monoclonal antibody (M00181-1)?
Verified Customer
Verified customer
Asked: 2018-05-03
Answer
We do provide the blocking peptide for product anti-Bcl-XL Rabbit Monoclonal antibody (M00181-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-05-03
Question
We want using your anti-Bcl-XL Rabbit Monoclonal antibody for positive regulation of intrinsic apoptotic signaling pathway studies. Has this antibody been tested with western blotting on k562 cell lysate? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2017-08-08
Answer
Thanks for your inquiry. This M00181-1 anti-Bcl-XL Rabbit Monoclonal antibody is tested on k562 cell lysate. It is guaranteed to work for IP, IF, IHC, WB in human, mouse, rat. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-08-08
Question
I was wanting to use your anti-Bcl-XL Rabbit Monoclonal antibody for WB for rat lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat lung identification?
L. Carter
Verified customer
Asked: 2016-03-31
Answer
You can see on the product datasheet, M00181-1 anti-Bcl-XL Rabbit Monoclonal antibody has been tested for IP, IF, IHC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-03-31
Question
Would anti-Bcl-XL Rabbit Monoclonal antibody M00181-1 work for WB with lung?
C. Miller
Verified customer
Asked: 2014-02-17
Answer
According to the expression profile of lung, BCL2L1 is highly expressed in lung. So, it is likely that anti-Bcl-XL Rabbit Monoclonal antibody M00181-1 will work for WB with lung.
Boster Scientific Support
Answered: 2014-02-17
Question
My boss were satisfied with the WB result of your anti-Bcl-XL Rabbit Monoclonal antibody. However we have observed positive staining in upper lobe of left lung isoform bcl-x(l): mitochondrion inner using this antibody. Is that expected? Could you tell me where is BCL2L1 supposed to be expressed?
M. Thomas
Verified customer
Asked: 2013-10-30
Answer
Based on literature, upper lobe of left lung does express BCL2L1. Generally BCL2L1 expresses in isoform bcl-x(l): mitochondrion inner. Regarding which tissues have BCL2L1 expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 17974005
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2013-10-30
