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Product Info Summary
| SKU: | EK0372 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Rat ICAM-1 ELISA Kit PicoKine®
View all ICAM-1/CD54 ELISA kits
SKU/Catalog Number
EK0372
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat ICAM-1 ELISA Kit PicoKine® (96 Tests). Quantitate Rat ICAM1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat ICAM-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0372)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q28-T493
Sensitivity
<2 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0372 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0372 is reactive to ICAM1 in Rat samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0372 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of ICAM-1/CD54
Intercellular adhesion molecule-1 (ICAM-1) is an integral membrane protein, a member of the immunoglobulin superfamily, and a ligand for lymphocyte function-associated (LFA) antigens, a beta 2 leukocyte integrin. The normal function of human intercellular adhesion molecule-1 (ICAM-1) is to provide adhesion between endothelial cells and leukocytes after injury or stress. ICAM-1 binds to leukocyte function-associated antigen (LFA-1) or macrophage-1 antigen (Mac-1). ICAM-1 is found on leukocytes, fibroblasts, epithelial cells and endothelial cells and its expression is regulated by inflammatory cytokines. ICAM-1 has a tissue distribution similar to that of the major histocompatibility complex class II antigens and is likely to play a role in inflammatory responses. The standard product used in this kit is produced by recombinant gene expression, which is a dimeric protein linked with disulfide bonds.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0372-CAP | Anti-Rat ICAM1 Pre-coated 96-well strip microplate | 1 |
| EK0372-ST | Rat ICAM1 Standard | 2 vials, 10 ng/tube |
| EK0372-DA | Rat ICAM1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.003 | 0.082 | 0.140 | 0.298 | 0.553 | 0.967 | 1.427 | 2.142 |
Data Example Images
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Rat ICAM-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect ICAM-1/CD54, Dilution ratio of 1:100, concentration in serum and plasma is around 8-58 ng/ml..
Some articles we found to cite concentrations of ICAM-1/CD54 in samples: 25112093 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat ICAM-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 51 | 231 | 787 | 51 | 242 | 855 |
| Standard deviation | 3.67 | 14.55 | 42.49 | 4.23 | 16.21 | 51.3 |
| CV (%) | 7.2% | 6.7% | 5.4% | 8.3% | 6.7% | 6% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of ICAM-1/CD54 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 51 | 47 | 49 | 56 | 50 | 3.34 | 6.6% |
| Sample 2 | 231 | 227 | 275 | 247 | 245 | 18.86 | 7.6% |
| Sample 3 | 787 | 761 | 862 | 797 | 801 | 37.18 | 4.6% |
Protein Target Info & Infographic
Gene/Protein Information For ICAM1 (Source: Uniprot.Org, NCBI)
Gene Name
ICAM1
Full Name
Intercellular adhesion molecule 1
Weight
57825 MW
Superfamily
immunoglobulin superfamily
Alternative Names
Intercellular adhesion molecule 1;ICAM-1;Major group rhinovirus receptor;CD54;ICAM1; ICAM1 BB2, CD54, P3.58 intercellular adhesion molecule 1 intercellular adhesion molecule 1|ICAM-1|cell surface glycoprotein P3.58|epididymis secretory sperm binding protein|intercellular adhesion molecule 1 (CD54), human rhinovirus receptor|major group rhinovirus receptor
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on ICAM1, check out the ICAM1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ICAM1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Rat ICAM-1 ELISA Kit PicoKine® (EK0372)
Hello CJ!
EK0372 has been cited in 38 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Exogenous α-calcitonin gene-related peptide attenuates lipopolysaccharide-induced acute lung injury in rats
Apple Polyphenols Decrease Atherosclerosis and Hepatic Steatosis in ApoE−/− Mice through the ROS/MAPK/NF-κB Pathway
RAGE Deletion Confers Renoprotection by Reducing Responsiveness to Transforming Growth Factor-β and Increasing Resistance to Apoptosis
Recombinant HDL (Milano) protects endotoxin-challenged rats from multiple organ injury and dysfunction
Alpha 1-antitrypsin ameliorates ventilator-induced lung injury in rats by inhibiting inflammatory responses and apoptosis:
Effects of hypercapnia on T cells in lung ischemia/reperfusion injury after lung transplantation:
Diannexin Can Ameliorate Acute Respiratory Distress Syndrome in Rats by Promoting Heme Oxygenase-1 Expression
Berberine Hydrochloride Prevents Postsurgery Intestinal Adhesion and Inflammation in Rats
Overexpression of Vasostatin‐1 Protects Hypoxia/Reoxygenation Injuries in Cardiomyocytes Independent of Endothelial Cells
Urocortin promotes the development of vasculitis in a rat model of thromboangiitis obliterans via corticotrophin‐releasing factor type 1 receptors
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Customer Reviews
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Customer Q&As
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4 Customer Q&As for Rat ICAM-1 ELISA Kit PicoKine®
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-07-05
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-07-05
Question
Q: if the enzyme conjugated CD54 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD54 antigen?
H. Gonzalez
Verified customer
Asked: 2019-04-14
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-04-14
Question
Q: can I use citrate plasma as samples in Rat CD54 Picokine® ELISA Kit (Catalog # EK0372)?
Verified Customer
Verified customer
Asked: 2018-08-08
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of CD54 causing disruption of its protein structure. CD54 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the CD54 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-08-08
Question
Q: how do I analyze ELISA data? I measured CD54 level in plasma.
Verified Customer
Verified customer
Asked: 2014-06-05
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2014-06-05
