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Product Info Summary
| SKU: | A00181-Dyl488 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | Flow Cytometry |
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Product info
Product Name
Anti-Human Bcl-XL DyLight® 488 conjugated BCL2L1 Antibody
SKU/Catalog Number
A00181-Dyl488
Size
100 μg/vial
Form
Liquid
Description
Boster Bio Anti-Human Bcl-XL DyLight® 488 conjugated BCL2L1 Antibody catalog # A00181-Dyl488. Tested in Flow Cytometry applications. This antibody reacts with Human.
Storage & Handling
At -20°C for one year from date of receipt. Avoid repeated freezing and thawing. Protect from light.
Cite This Product
Anti-Human Bcl-XL DyLight® 488 conjugated BCL2L1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00181-Dyl488)
Host
Rabbit
Contents
Each vial contains 50% glycerol, 0.9% NaCl, 0.2% Na2HPO4, 0.02% NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Bcl-XL recombinant protein (Position: M1-T219). Human Bcl-XL shares 97.9% amino acid (aa) sequence identity with both mouse and rat Bcl-XL.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00181-Dyl488 is reactive to BCL2L1 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
26.049kDa
Background of BCL2L1
Bcl-2-like protein 1, also known as Bcl-XL, is a protein that in humans is encoded by the BCL2L1 gene. The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform (Bcl-xL) acts as an apoptotic inhibitor and the shorter form (Bcl-xS) acts as an apoptotic activator.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00181-Dyl488 is guaranteed for Flow Cytometry Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow Cytometry (Fixed), 1-3μg/1x106 cells
Positive Control
FCM: PC-3 cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Flow Cytometry analysis of PC-3 cells using anti-Human Bcl-XL antibody (A00181-Dyl488).
Overlay histogram showing PC-3 cells stained with A00181-Dyl488 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Human Bcl-XL Antibody (A00181-Dyl488,1μg/1x106 cells) for 30 min at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For BCL2L1 (Source: Uniprot.org, NCBI)
Gene Name
BCL2L1
Full Name
Bcl-2-like protein 1
Weight
26.049kDa
Superfamily
Bcl-2 family
Alternative Names
Bcl-2-like protein 1; Bcl2-L-1; Apoptosis regulator Bcl-X; BCL2L1; BCL2L; BCLX BCL2L1 BCL-XL/S, BCL2L, BCLX, Bcl-X, PPP1R52 BCL2 like 1 bcl-2-like protein 1|apoptosis regulator Bcl-X|protein phosphatase 1, regulatory subunit 52
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BCL2L1, check out the BCL2L1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BCL2L1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Human Bcl-XL DyLight® 488 conjugated BCL2L1 Antibody (A00181-Dyl488)
Hello CJ!
A00181-Dyl488 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Relationship between Egr-1 gene expression and apoptosis in esophageal carcinoma and precancerous lesions
Emodin alleviates severe acute pancreatitis-associated acute lung injury by decreasing pre-B-cell colony-enhancing factor expression and promoting polymorphonuclear neutrophil apoptosis
Zhou X, Zhang Y, Li Y, Hao X, Liu X, Wang Y. Cancers (Basel). 2012 Dec 4;4(4):1318-32. Doi: 10.3390/Cancers4041318. Azithromycin Synergistically Enhances Anti-Proliferative Activity Of Vincristine In Cervical And Gastric Cancer Cells.
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Customer Reviews
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Customer Q&As
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14 Customer Q&As for Anti-Human Bcl-XL DyLight® 488 conjugated BCL2L1 Antibody
Question
My question regarding product A00181-Dyl488, anti-Human Bcl-XL DyLight® 488 conjugated antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
E. Anderson
Verified customer
Asked: 2020-02-28
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00181-Dyl488 anti-Human Bcl-XL DyLight® 488 conjugated antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-28
Question
Thanks for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for colon carcinoma using anti-Human Bcl-XL DyLight® 488 conjugated antibody A00181-Dyl488. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-24
Question
Is there a BSA free version of anti-Human Bcl-XL DyLight® 488 conjugated antibody A00181-Dyl488 available?
Verified Customer
Verified customer
Asked: 2019-10-24
Answer
I appreciate your recent telephone inquiry. I can confirm that some lots of this anti-Human Bcl-XL DyLight® 488 conjugated antibody A00181-Dyl488 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-24
Question
Is this A00181-Dyl488 anti-Human Bcl-XL DyLight® 488 conjugated antibody reactive to the isotypes of BCL2L1?
Verified Customer
Verified customer
Asked: 2019-10-15
Answer
The immunogen of A00181-Dyl488 anti-Human Bcl-XL DyLight® 488 conjugated antibody is E.coli-derived human Bcl-XL recombinant protein (Position: M1-T219). Human Bcl-XL shares 97.9% amino acid (aa) sequence identity with both mouse and rat Bcl-XL. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-10-15
Question
We are currently using anti-Human Bcl-XL DyLight® 488 conjugated antibody A00181-Dyl488 for human tissue, and we are content with the Flow Cytometry results. The species of reactivity given in the datasheet says human. Is it true that the antibody can work on pig tissues as well?
C. Baker
Verified customer
Asked: 2019-09-20
Answer
The anti-Human Bcl-XL DyLight® 488 conjugated antibody (A00181-Dyl488) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-09-20
Question
We have observed staining in human upper lobe of left lung. Are there any suggestions? Is anti-Human Bcl-XL DyLight® 488 conjugated antibody supposed to stain upper lobe of left lung positively?
Verified Customer
Verified customer
Asked: 2019-08-14
Answer
From literature upper lobe of left lung does express BCL2L1. From Uniprot.org, BCL2L1 is expressed in upper lobe of left lung, colon carcinoma, lung, among other tissues. Regarding which tissues have BCL2L1 expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 17974005
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2019-08-14
Question
Does A00181-Dyl488 anti-Human Bcl-XL DyLight® 488 conjugated antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-05-29
Answer
It shows on the product datasheet, A00181-Dyl488 anti-Human Bcl-XL DyLight® 488 conjugated antibody as been tested on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-05-29
Question
I see that the anti-Human Bcl-XL DyLight® 488 conjugated antibody A00181-Dyl488 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-09-04
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-09-04
Question
Is a blocking peptide available for product anti-Human Bcl-XL DyLight® 488 conjugated antibody (A00181-Dyl488)?
Verified Customer
Verified customer
Asked: 2018-03-05
Answer
We do provide the blocking peptide for product anti-Human Bcl-XL DyLight® 488 conjugated antibody (A00181-Dyl488). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2018-03-05
Question
My boss were content with the WB result of your anti-Human Bcl-XL DyLight® 488 conjugated antibody. However we have observed positive staining in upper lobe of left lung isoform bcl-x(l): mitochondrion inner using this antibody. Is that expected? Could you tell me where is BCL2L1 supposed to be expressed?
L. Collins
Verified customer
Asked: 2016-09-15
Answer
From literature, upper lobe of left lung does express BCL2L1. Generally BCL2L1 expresses in isoform bcl-x(l): mitochondrion inner. Regarding which tissues have BCL2L1 expression, here are a few articles citing expression in various tissues:
Colon carcinoma, Pubmed ID: 17974005
Lung, Pubmed ID: 15489334
Boster Scientific Support
Answered: 2016-09-15
Question
We want to test anti-Human Bcl-XL DyLight® 488 conjugated antibody A00181-Dyl488 on human colon carcinoma for research purposes, then I may be interested in using anti-Human Bcl-XL DyLight® 488 conjugated antibody A00181-Dyl488 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
L. Anderson
Verified customer
Asked: 2015-06-17
Answer
The products we sell, including anti-Human Bcl-XL DyLight® 488 conjugated antibody A00181-Dyl488, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2015-06-17
Question
I was wanting to use your anti-Human Bcl-XL DyLight® 488 conjugated antibody for Flow Cytometry for human colon carcinoma on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human colon carcinoma identification?
C. Evans
Verified customer
Asked: 2015-02-04
Answer
As indicated on the product datasheet, A00181-Dyl488 anti-Human Bcl-XL DyLight® 488 conjugated antibody has been validated for Flow Cytometry on human tissues. We have an innovator award program that if you test this antibody and show it works in human colon carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-02-04
Question
Does anti-Human Bcl-XL DyLight® 488 conjugated antibody A00181-Dyl488 work for Flow Cytometry with colon carcinoma?
D. Johnson
Verified customer
Asked: 2014-12-16
Answer
According to the expression profile of colon carcinoma, BCL2L1 is highly expressed in colon carcinoma. So, it is likely that anti-Human Bcl-XL DyLight® 488 conjugated antibody A00181-Dyl488 will work for Flow Cytometry with colon carcinoma.
Boster Scientific Support
Answered: 2014-12-16
Question
I have attached the WB image, lot number and protocol we used for colon carcinoma using anti-Human Bcl-XL DyLight® 488 conjugated antibody A00181-Dyl488. Please let me know if you require anything else.
T. Taylor
Verified customer
Asked: 2013-08-06
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-08-06
