Anti-BARD1 Antibody

Product Name

Anti-BARD1 Antibody

View all BARD1 Antibodies

SKU/Catalog Number

A01646

Size

100 µl

Form

Liquid

Description

Boster Bio Anti-BARD1 Antibody (Catalog # A01646). Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-BARD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01646)

Host

Rabbit

Contents

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Clonality

Polyclonal

Isotype

IgG

Immunogen

Synthesized peptide derived from N-terminal of human BARD1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross reactivity with other proteins.

Reactive Species

A01646 is reactive to BARD1 in Human

Reconstitution

Calculated molecular weight

86648 MW

Background of BARD1

Probable E3 ubiquitin-protein ligase. The BRCA1-BARD1 heterodimer specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Plays a central role in the control of the cell cycle in response to DNA damage. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Also forms a heterodimer with CSTF1/CSTF-50 to modulate mRNA processing and RNAP II stability by inhibiting pre-mRNA 3' cleavage.

Wu L.C., Nat. Genet. 14:430-440(1996).
Thai T.H., Hum. Mol. Genet. 7:195-202(1998).
Hillier L.W., Nature 434:724-731(2005).

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01646 is guaranteed for WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blotting: 1:500~1:3000

Validation Images & Assay Conditions

Gene/Protein Information For BARD1 (Source: Uniprot.org, NCBI)

Alternative Names

BRCA1-associated RING domain protein 1;BARD-1;6.3.2.-;BARD1; BARD1 BRCA1 associated RING domain 1 BRCA1-associated RING domain protein 1|BRCA1-associated RING domain gene 1|RING-type E3 ubiquitin transferase BARD1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

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