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Product Info Summary
| SKU: | A00005 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IF, ICC, WB |
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Product info
Product Name
Anti-BRCA1 Antibody Picoband®
SKU/Catalog Number
A00005
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-BRCA1 Antibody Picoband® catalog # A00005. Tested in ICC, IF, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BRCA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00005)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human BRCA1 recombinant protein (Position: T1681-E1781). Human BRCA1 shares 73.3% and 74.3% amino acid (aa) sequence identity with mouse and rat BRCA1, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00005 is reactive to BRCA1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
290 kDa
Calculated molecular weight
207721 MW
Background of BRCA1
BRCA1, mapped to 17q21.3, is also known as BRCC1. This gene encodes a nuclear phosphoprotein that plays a role in maintaining genomic stability, and it also acts as a tumor suppressor. The encoded protein combines with other tumor suppressors, DNA damage sensors, and signal transducers to form a large multi-subunit protein complex known as the BRCA1-associated genome surveillance complex (BASC). BRCA1 product associates with RNA polymerase II, and through the C-terminal domain, also interacts with histone deacetylase complexes. This protein thus plays a role in transcription, DNA repair of double-stranded breaks, and recombination. In addition to it, BRCA1 may normally serve as a negative regulator of mammary epithelial cell growth and that this function is compromised in breast cancer either by direct mutation or by alterations in gene expression.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00005 is guaranteed for IF, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Positive Control
WB: human Hela whole cell, human MCF-7 whole cell, human T47D whole cell, human CACO-2 whole cell, rat C6 whole cell, mouse Neuro-2a whole cell
ICC/IF: U2OS cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of BRCA1 using anti-BRCA1 antibody (A00005).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: human T47D whole cell lysates,
Lane 4: human CACO-2 whole cell lysates,
Lane 5: rat C6 whole cell lysates,
Lane 6: mouse Neuro-2a whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRCA1 antigen affinity purified polyclonal antibody (Catalog # A00005) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for BRCA1 at approximately 290 kDa. The expected band size for BRCA1 is at 208 kDa.
Click image to see more details
Figure 2. IF analysis of BRCA1 using anti-BRCA1 antibody (A00005) and anti-Beta Tubulin antibody (M01857-3).
BRCA1 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 μg/mL rabbit anti-BRCA1 Antibody (A00005) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4°C. DyLight®488 Conjugated Donkey Anti-Rabbit IgG (BA1144) and DyLight®594 Conjugated Goat Anti-Mouse IgG (BA1141) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
Protein Target Info & Infographic
Gene/Protein Information For BRCA1 (Source: Uniprot.org, NCBI)
Gene Name
BRCA1
Full Name
Breast cancer type 1 susceptibility protein
Weight
207721 MW
Alternative Names
Breast cancer type 1 susceptibility protein;6.3.2.-;RING finger protein 53;BRCA1;RNF53; BRCA1 BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53 BRCA1 DNA repair associated breast cancer type 1 susceptibility protein|BRCA1/BRCA2-containing complex, subunit 1|Fanconi anemia, complementation group S|RING finger protein 53|breast and ovarian cancer susceptibility protein 1|breast cancer 1, early onset|early onset breast cancer 1|protein phosphatase 1, regulatory subunit 53
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BRCA1, check out the BRCA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BRCA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BRCA1 Antibody Picoband® (A00005)
Hello CJ!
A00005 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zhang Q, Zhang Q, Cong H, Zhang X. Diagn Pathol. 2012 Dec 31;7:181. Doi: 10.1186/1746-1596-7-181. The Ectopic Expression Of Brca1 Is Associated With Genesis, Progression, And Prognosis Of Breast Cancer In Young Patients.
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Customer Q&As
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15 Customer Q&As for Anti-BRCA1 Antibody Picoband®
Question
Is a blocking peptide available for product anti-BRCA1 antibody (A00005)?
Verified Customer
Verified customer
Asked: 2020-04-29
Answer
We do provide the blocking peptide for product anti-BRCA1 antibody (A00005). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-29
Question
We have seen staining in human pns. Any tips? Is anti-BRCA1 antibody supposed to stain pns positively?
Verified Customer
Verified customer
Asked: 2020-01-30
Answer
According to literature pns does express BRCA1. According to Uniprot.org, BRCA1 is expressed in lung, mammary gland, testis, pns, cervix carcinoma, embryonic kidney, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have BRCA1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Mammary gland, Pubmed ID: 9010228
PNS, Pubmed ID: 15489334
Testis, Pubmed ID: 16625196
Boster Scientific Support
Answered: 2020-01-30
Question
Will anti-BRCA1 antibody A00005 work for WB with lung?
Verified Customer
Verified customer
Asked: 2019-10-03
Answer
According to the expression profile of lung, BRCA1 is highly expressed in lung. So, it is likely that anti-BRCA1 antibody A00005 will work for WB with lung.
Boster Scientific Support
Answered: 2019-10-03
Question
My lab would like to test anti-BRCA1 antibody A00005 on human lung for research purposes, then I may be interested in using anti-BRCA1 antibody A00005 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-09-04
Answer
The products we sell, including anti-BRCA1 antibody A00005, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-04
Question
Would A00005 anti-BRCA1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-09-03
Answer
It shows on the product datasheet, A00005 anti-BRCA1 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-09-03
Question
I am interested in using your anti-BRCA1 antibody for dna-templated studies. Has this antibody been tested with western blotting on skov3 whole cell lysates? We would like to see some validation images before ordering.
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
Thanks for your inquiry. This A00005 anti-BRCA1 antibody is tested on hela whole cell lysates, skov3 whole cell lysates. It is guaranteed to work for WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2019-08-29
Question
I have a question about product A00005, anti-BRCA1 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-07-09
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00005 anti-BRCA1 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-07-09
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for lung using anti-BRCA1 antibody A00005. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-16
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-16
Question
I was wanting to use your anti-BRCA1 antibody for WB for human lung on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human lung identification?
Verified Customer
Verified customer
Asked: 2018-09-24
Answer
As indicated on the product datasheet, A00005 anti-BRCA1 antibody has been tested for WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human lung in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2018-09-24
Question
My colleagues were satisfied with the WB result of your anti-BRCA1 antibody. However we have observed positive staining in embryonic kidney nucleus using this antibody. Is that expected? Could you tell me where is BRCA1 supposed to be expressed?
G. Wu
Verified customer
Asked: 2017-08-15
Answer
From what I have seen in literature, embryonic kidney does express BRCA1. Generally BRCA1 expresses in nucleus. Regarding which tissues have BRCA1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Mammary gland, Pubmed ID: 9010228
PNS, Pubmed ID: 15489334
Testis, Pubmed ID: 16625196
Boster Scientific Support
Answered: 2017-08-15
Question
I see that the anti-BRCA1 antibody A00005 works with WB, what is the protocol used to produce the result images on the product page?
F. Krishna
Verified customer
Asked: 2016-11-14
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2016-11-14
Question
Do you have a BSA free version of anti-BRCA1 antibody A00005 available?
D. Bhatt
Verified customer
Asked: 2016-03-23
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-BRCA1 antibody A00005 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-03-23
Question
We are currently using anti-BRCA1 antibody A00005 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on primate tissues as well?
A. Walker
Verified customer
Asked: 2015-04-24
Answer
The anti-BRCA1 antibody (A00005) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-04-24
Question
Is this A00005 anti-BRCA1 antibody reactive to the isotypes of BRCA1?
K. Johnson
Verified customer
Asked: 2015-03-16
Answer
The immunogen of A00005 anti-BRCA1 antibody is E.coli-derived human BRCA1 recombinant protein (Position: T1681-E1781). Human BRCA1 shares 73.3% and 74.3% amino acid (aa) sequence identity with mouse and rat BRCA1, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-03-16
Question
I have attached the WB image, lot number and protocol we used for lung using anti-BRCA1 antibody A00005. Please let me know if you require anything else.
S. Banerjee
Verified customer
Asked: 2013-12-24
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-12-24
