Product Info Summary
SKU: | PA1557 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IHC, WB |
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Product info
Product Name
Anti-Cullin 1/Cul1 Antibody Picoband®
SKU/Catalog Number
PA1557
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Cullin 1/Cul1 Antibody catalog # PA1557. Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Cullin 1/Cul1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1557)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human Cullin 1, identical to the related mouse and rat sequences.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PA1557 is reactive to CUL1 in Human, Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
90 kDa
Calculated molecular weight
89679 MW
Background of Cullin 1
Cullin 1, also known as CUL1, is a human protein and gene from cullin family. This protein plays an important role in protein degradation and protein ubiquitination. This is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. Human cullin 1, but not the other closely related cullins 2, 3, 4A, and 5, selectively interacts with human SKP1.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PA1557 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Positive Control
WB: MM453 Whole Cell, HT1080 Whole Cell, SMMC Whole Cell
IHC: Human Lung Cancer Tissue
Validation Images & Assay Conditions
Click image to see more details
Anti-Cullin 1 antibody, PA1557, Western blotting
All lanes: Anti Cullin 1 (PA1557) at 0.5ug/ml
Lane 1: MM453 Whole Cell Lysate at 40ug
Lane 2: HT1080 Whole Cell Lysate at 40ug
Lane 3: SMMC Whole Cell Lysate at 40ug
Predicted bind size: 90 KD
Observed bind size: 90 KD
Click image to see more details
Anti-Cullin 1 antibody, PA1557, IHC(P)
IHC(P): Human Lung Cancer Tissue
Protein Target Info & Infographic
Gene/Protein Information For CUL1 (Source: Uniprot.org, NCBI)
Gene Name
CUL1
Full Name
Cullin-1
Weight
89679 MW
Superfamily
cullin family
Alternative Names
Cullin-1;CUL-1;CUL1; CUL1 cullin 1 cullin-1|CUL-1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on CUL1, check out the CUL1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for CUL1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Cullin 1/Cul1 Antibody Picoband® (PA1557)
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