Click image to see more details
Product Info Summary
| SKU: | A00005-1 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | IF, WB |
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Product info
Product Name
Anti-BRCA1 (Ab-1524) Antibody
SKU/Catalog Number
A00005-1
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-BRCA1 (Ab-1524) Antibody (Catalog # A00005-1). Tested in WB, IF applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-BRCA1 (Ab-1524) Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00005-1)
Host
Rabbit
Contents
Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Peptide sequence around aa.1522~1526 (Y-P-S-Q-E) derived from Human BRCA1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A00005-1 is reactive to BRCA1 in Human
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
288892 MW
Background of BRCA1
The BRCA1-BARD1 heterodimer coordinates a diverse range of cellular pathways such as DNA damage repair, ubiquitination and transcriptional regulation to maintain genomic stability. Acts by mediating ubiquitin E3 ligase activity that is required for its tumor suppressor function. Plays a central role in DNA repair by facilitating cellular response to DNA repair. Required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Involved in transcriptional regulation of P21 in response to DNA damage. Required for FANCD2 targeting to sites of DNA damage. May function as a transcriptional regulator. Inhibits lipid synthesis by binding to inactive phosphorylated ACACA and preventing its dephosphorylation
Lin SY, et al. (2004) Proc Natl Acad Sci U S A, 101(17): 6484-6489
Zhang J, et al. (2004) Mol Cell Biol; 24(2): 708-718
Beger C, et al. (2001) Proc Natl Acad Sci U S A; 98(1): 130-135
Gardner K, et al. (2001) Breast Cancer Res; 3(1): 11-13
Zheng L, et al. (2001) Proc Natl Acad Sci U S A; 98(17): 9587-9592
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A00005-1 is guaranteed for IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Predicted MW: 220kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of BRCA1 using anti-BRCA1 antibody (A00005-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRCA1 antigen affinity purified polyclonal antibody (Catalog # A00005-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1022) with Tanon 5200 system. A specific band was detected for BRCA1.
Click image to see more details
Immunofluorescence staining of methanol-fixed Hela cells using BRCA1(Ab-1524) Antibody #A00005-1.
Protein Target Info & Infographic
Gene/Protein Information For BRCA1 (Source: Uniprot.org, NCBI)
Gene Name
BRCA1
Full Name
Breast cancer type 1 susceptibility protein
Weight
288892 MW
Alternative Names
Serine/threonine-protein kinase mTOR;2.7.11.1;FK506-binding protein 12-rapamycin complex-associated protein 1;FKBP12-rapamycin complex-associated protein;Mammalian target of rapamycin;mTOR;Mechanistic target of rapamycin;Rapamycin and FKBP12 target 1;Rapamycin target protein 1;MTOR;FRAP, FRAP1, FRAP2, RAFT1, RAPT1; BRCA1 BRCAI, BRCC1, BROVCA1, FANCS, IRIS, PNCA4, PPP1R53, PSCP, RNF53 BRCA1 DNA repair associated breast cancer type 1 susceptibility protein|BRCA1/BRCA2-containing complex, subunit 1|Fanconi anemia, complementation group S|RING finger protein 53|breast and ovarian cancer susceptibility protein 1|breast cancer 1, early onset|early onset breast cancer 1|protein phosphatase 1, regulatory subunit 53
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on BRCA1, check out the BRCA1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for BRCA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-BRCA1 (Ab-1524) Antibody (A00005-1)
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Customer Q&As
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16 Customer Q&As for Anti-BRCA1 (Ab-1524) Antibody
Question
I was wanting to use your anti-BRCA1 (Ab-1524) antibody for WB for human testis on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human testis identification?
Verified Customer
Verified customer
Asked: 2020-03-26
Answer
As indicated on the product datasheet, A00005-1 anti-BRCA1 (Ab-1524) antibody has been validated for IF, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human testis in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-26
Question
My colleagues were content with the WB result of your anti-BRCA1 (Ab-1524) antibody. However we have observed positive staining in cervix carcinoma erythroleukemia nucleus using this antibody. Is that expected? Could you tell me where is BRCA1 supposed to be expressed?
Verified Customer
Verified customer
Asked: 2020-03-09
Answer
According to literature, cervix carcinoma erythroleukemia does express BRCA1. Generally BRCA1 expresses in nucleus. Regarding which tissues have BRCA1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Mammary gland, Pubmed ID: 9010228
PNS, Pubmed ID: 15489334
Testis, Pubmed ID: 16625196
Boster Scientific Support
Answered: 2020-03-09
Question
Is there a BSA free version of anti-BRCA1 (Ab-1524) antibody A00005-1 available?
Verified Customer
Verified customer
Asked: 2019-11-14
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-BRCA1 (Ab-1524) antibody A00005-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-11-14
Question
My question regarding product A00005-1, anti-BRCA1 (Ab-1524) antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-05
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A00005-1 anti-BRCA1 (Ab-1524) antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-05
Question
Is a blocking peptide available for product anti-BRCA1 (Ab-1524) antibody (A00005-1)?
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
We do provide the blocking peptide for product anti-BRCA1 (Ab-1524) antibody (A00005-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-08-01
Question
Our lab used your anti-BRCA1 (Ab-1524) antibody for IF on leukemic t-cell last year. I am using human, and We are going to use the antibody for WB next. My lab would like examining leukemic t-cell as well as mammary gland in our next experiment. Could you please give me some suggestion on which antibody would work the best for WB?
Verified Customer
Verified customer
Asked: 2019-04-11
Answer
I took a look at the website and datasheets of our anti-BRCA1 (Ab-1524) antibody and I see that A00005-1 has been tested on human in both IF and WB. Thus A00005-1 should work for your application. Our Boster satisfaction guarantee will cover this product for WB in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for WB detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-04-11
Question
We have seen staining in human cervix carcinoma. Are there any suggestions? Is anti-BRCA1 (Ab-1524) antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2018-10-02
Answer
According to literature cervix carcinoma does express BRCA1. According to Uniprot.org, BRCA1 is expressed in lung, mammary gland, testis, pns, cervix carcinoma, embryonic kidney, leukemic t-cell, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have BRCA1 expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 17081983, 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Embryonic kidney, Pubmed ID: 17525332
Leukemic T-cell, Pubmed ID: 19690332
Mammary gland, Pubmed ID: 9010228
PNS, Pubmed ID: 15489334
Testis, Pubmed ID: 16625196
Boster Scientific Support
Answered: 2018-10-02
Question
I am interested in using your anti-BRCA1 (Ab-1524) antibody for double-strand break repair via nonhomologous end joining studies. Has this antibody been tested with western blotting on hela cells? We would like to see some validation images before ordering.
P. Lewis
Verified customer
Asked: 2018-08-02
Answer
I appreciate your inquiry. This A00005-1 anti-BRCA1 (Ab-1524) antibody is validated on hela cells. It is guaranteed to work for IF, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2018-08-02
Question
We are currently using anti-BRCA1 (Ab-1524) antibody A00005-1 for human tissue, and we are happy with the IF results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on primate tissues as well?
Verified Customer
Verified customer
Asked: 2018-07-06
Answer
The anti-BRCA1 (Ab-1524) antibody (A00005-1) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2018-07-06
Question
Is this A00005-1 anti-BRCA1 (Ab-1524) antibody reactive to the isotypes of BRCA1?
Verified Customer
Verified customer
Asked: 2018-06-12
Answer
The immunogen of A00005-1 anti-BRCA1 (Ab-1524) antibody is Peptide sequence around aa.1522~1526 (Y-P-S-Q-E) derived from Human BRCA1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2018-06-12
Question
I am interested in to test anti-BRCA1 (Ab-1524) antibody A00005-1 on human testis for research purposes, then I may be interested in using anti-BRCA1 (Ab-1524) antibody A00005-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
D. Baker
Verified customer
Asked: 2017-10-13
Answer
The products we sell, including anti-BRCA1 (Ab-1524) antibody A00005-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2017-10-13
Question
I see that the anti-BRCA1 (Ab-1524) antibody A00005-1 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-09-12
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-09-12
Question
I appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for testis using anti-BRCA1 (Ab-1524) antibody A00005-1. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2017-05-17
Answer
I appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-05-17
Question
Would anti-BRCA1 (Ab-1524) antibody A00005-1 work for WB with testis?
N. Patel
Verified customer
Asked: 2017-05-09
Answer
According to the expression profile of testis, BRCA1 is highly expressed in testis. So, it is likely that anti-BRCA1 (Ab-1524) antibody A00005-1 will work for WB with testis.
Boster Scientific Support
Answered: 2017-05-09
Question
I have attached the WB image, lot number and protocol we used for testis using anti-BRCA1 (Ab-1524) antibody A00005-1. Please let me know if you require anything else.
S. Krishna
Verified customer
Asked: 2014-02-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2014-02-03
Question
Will A00005-1 anti-BRCA1 (Ab-1524) antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
D. Li
Verified customer
Asked: 2013-07-22
Answer
You can see on the product datasheet, A00005-1 anti-BRCA1 (Ab-1524) antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2013-07-22
