Product Info Summary
SKU: | A03993 |
---|---|
Size: | 100 µl |
Reactive Species: | Human, Mouse |
Host: | Rabbit |
Application: | WB |
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Product info
Product Name
Anti-B4GALT1/Beta 1 4 Gal T1 Antibody
View all beta-1,4-Galactosyltransferase 1/B4GalT1 Antibodies
SKU/Catalog Number
A03993
Size
100 µl
Form
Liquid
Description
Boster Bio Anti-B4GALT1/Beta 1 4 Gal T1 Antibody (Catalog # A03993). Tested in WB applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-B4GALT1/Beta 1 4 Gal T1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03993)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from C-terminal of human B4GALT1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A03993 is reactive to B4GALT1 in Human, Mouse
Reconstitution
Calculated molecular weight
43920 MW
Background of beta-1,4-Galactosyltransferase 1/B4GalT1
The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. The cell surface form functions as a recognition molecule during a variety of cell to cell and cell to matrix interactions, as those occurring during development and egg fertilization, by binding to specific oligosaccharide ligands on opposing cells or in the extracellular matrix.
Masri K.A., Biochem. Biophys. Res. Commun. 157:657-663(1988).
Watzele G., Nucleic Acids Res. 18:7174-7174(1990).
Mengle-Gaw L., Biochem. Biophys. Res. Commun. 176:1269-1276(1991).
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03993 is guaranteed for WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blotting: 1:500~1:3000
Validation Images & Assay Conditions
Click image to see more details
Western blot analysis of extracts from Jurkat/293 cells, using B4GALT1 antibody A03993.
Protein Target Info & Infographic
Gene/Protein Information For B4GALT1 (Source: Uniprot.org, NCBI)
Gene Name
B4GALT1
Full Name
Beta-1,4-galactosyltransferase 1
Weight
43920 MW
Superfamily
glycosyltransferase 7 family
Alternative Names
Beta-1,4-galactosyltransferase 1;Beta-1,4-GalTase 1;Beta4Gal-T1;b4Gal-T1;2.4.1.-;UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1;UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1;Lactose synthase A protein;2.4.1.22;N-acetyllactosamine synthase;2.4.1.90;Nal synthase;Beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase;2.4.1.38;Beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase;2.4.1.-;Processed beta-1,4-galactosyltransferase 1;B4GALT1;GGTB2; B4GALT1 B4GAL-T1, CDG2D, GGTB2, GT1, GTB, beta4Gal-T1 beta-1,4-galactosyltransferase 1 beta-1,4-galactosyltransferase 1|N-acetyllactosamine synthase|UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1|UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 1|UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1|beta-1,4-GalTase 1|beta-N-acetylglucosaminyl-glycolipid beta-1,4-galactosyltransferase|beta-N-acetylglucosaminylglycopeptide beta-1,4-galactosyltransferase|glycoprotein-4-beta-galactosyltransferase 2|lactose synthase A protein|nal synthase|neolactotriaosylceramide beta-1,4-galactosyltransferase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on B4GALT1, check out the B4GALT1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for B4GALT1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-B4GALT1/Beta 1 4 Gal T1 Antibody (A03993)
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Customer Q&As
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8 Customer Q&As for Anti-B4GALT1/Beta 1 4 Gal T1 Antibody
Question
Here is the WB image, lot number and protocol we used for liver using anti-B4GALT1/Beta 1 4 Gal T1 antibody A03993. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-09-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-09-05
Question
Does anti-B4GALT1/Beta 1 4 Gal T1 antibody A03993 work on primate WB with placenta?
Verified Customer
Verified customer
Asked: 2019-06-25
Answer
Our lab technicians have not tested anti-B4GALT1/Beta 1 4 Gal T1 antibody A03993 on primate. You can run a BLAST between primate and the immunogen sequence of anti-B4GALT1/Beta 1 4 Gal T1 antibody A03993 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate placenta in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-25
Question
Can A03993 antibody bind to CHO cells' b4GalT1?
Verified customer
Asked: 2019-04-18
Answer
Yes, our Anti-B4GALT1/Beta 1 4 Gal T1 Antibody A03993 binds to CHO cells' b4GalT1.
Boster Scientific Support
Answered: 2019-04-19
Question
I was wanting to use to test anti-B4GALT1/Beta 1 4 Gal T1 antibody A03993 on mouse liver for research purposes, then I may be interested in using anti-B4GALT1/Beta 1 4 Gal T1 antibody A03993 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2018-05-29
Answer
The products we sell, including anti-B4GALT1/Beta 1 4 Gal T1 antibody A03993, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2018-05-29
Question
Does anti-B4GALT1/Beta 1 4 Gal T1 antibody A03993 work for WB with liver?
Verified Customer
Verified customer
Asked: 2017-10-20
Answer
According to the expression profile of liver, B4GALT1 is highly expressed in liver. So, it is likely that anti-B4GALT1/Beta 1 4 Gal T1 antibody A03993 will work for WB with liver.
Boster Scientific Support
Answered: 2017-10-20
Question
We are currently using anti-B4GALT1/Beta 1 4 Gal T1 antibody A03993 for mouse tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on monkey tissues as well?
Verified Customer
Verified customer
Asked: 2017-09-21
Answer
The anti-B4GALT1/Beta 1 4 Gal T1 antibody (A03993) has not been tested for cross reactivity specifically with monkey tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-09-21
Question
I see that the anti-B4GALT1/Beta 1 4 Gal T1 antibody A03993 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-05-23
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-05-23
Question
Do you have a BSA free version of anti-B4GALT1/Beta 1 4 Gal T1 antibody A03993 available?
K. Jha
Verified customer
Asked: 2016-11-23
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-B4GALT1/Beta 1 4 Gal T1 antibody A03993 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2016-11-23