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Product Info Summary
| SKU: | PB9354 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Mouse, Rat |
| Host: | Rabbit |
| Application: | IHC, WB |
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Product info
Product Name
Anti-alpha 1 Antitrypsin/SERPINA1 Antibody Picoband®
View all Serpin A1/alpha 1-Antitrypsin Antibodies
SKU/Catalog Number
PB9354
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-alpha 1 Antitrypsin/SERPINA1 Antibody Picoband® catalog # PB9354. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-alpha 1 Antitrypsin/SERPINA1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9354)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived rat SERPINA1 recombinant protein (Position: K211-R411). Rat SERPINA1 shares 66% amino acid (aa) sequence identity with human SERPINA1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
PB9354 is reactive to Serpina1 in Mouse, Rat
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
53 kDa
Calculated molecular weight
46136 MW
Background of Serpin A1/alpha 1-Antitrypsin
SERPINA1 is also known as PI, A1A or AAT. The protein encoded by this gene is secreted and is a serine protease inhibitor whose targets include elastase, plasmin, thrombin, trypsin, chymotrypsin, and plasminogen activator. Defects in this gene can cause emphysema or liver disease. Several transcript variants encoding the same protein have been found for this gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
PB9354 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat, By Heat
Western blot, 0.1-0.5μg/ml, Mouse, Rat
Positive Control
WB: rat kidney tissue, rat lung tissue, rat testis tissue, mouse kidney tissue, mouse lung tissue
IHC: rat spleen tissue, mouse spleen tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of SERPINA1 using anti-SERPINA1 antibody (PB9354).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat kidney tissue lysates,
Lane 2: rat lung tissue lysates,
Lane 3: rat testis tissue lysates,
Lane 4: mouse kidney tissue lysates,
Lane 5: mouse lung tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SERPINA1 antigen affinity purified polyclonal antibody (Catalog # PB9354) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SERPINA1 at approximately 53 kDa. The expected band size for SERPINA1 is at 47 kDa.
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Figure 2. IHC analysis of SERPINA1 using anti-SERPINA1 antibody (PB9354).
SERPINA1 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SERPINA1 Antibody (PB9354) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
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Figure 3. IHC analysis of SERPINA1 using anti-SERPINA1 antibody (PB9354).
SERPINA1 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 μg/ml rabbit anti-SERPINA1 Antibody (PB9354) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For Serpina1 (Source: Uniprot.org, NCBI)
Gene Name
Serpina1
Full Name
Alpha-1-antitrypsin
Weight
46136 MW
Superfamily
serpin family
Alternative Names
Alpha-1-antiproteinase;Alpha-1-antitrypsin;Alpha-1-proteinase inhibitor;Serpin A1;Serpina1; SERPINA1 A1A, A1AT, AAT, PI, PI1, PRO2275, alpha1AT, nNIF serpin family A member 1 alpha-1-antitrypsin|alpha-1 antitrypsin|alpha-1 protease inhibitor|alpha-1-antiproteinase|alpha-1-antitrypsin short transcript variant 1C4|alpha-1-antitrypsin short transcript variant 1C5|epididymis secretory sperm binding protein|protease inhibitor 1 (anti-elastase), alpha-1-antitrypsin|serine (or cysteine) proteinase inhibitor, clade A, member 1|serpin A1|serpin peptidase inhibitor clade A (alpha-1antiproteinase, antitrypsin) member 1|serpin peptidase inhibitor clade A member 1|serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 1
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Serpina1, check out the Serpina1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Serpina1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-alpha 1 Antitrypsin/SERPINA1 Antibody Picoband® (PB9354)
Hello CJ!
PB9354 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Label-free proteomic analysis of placental proteins during Toxoplasma gondii infection
Lv X, Gao F, Liu Q, Zhang S, Huang Z, Zhu Y, Zong H, Li Q, Li S. Exp Ther Med. 2018 Feb;15(2):1465-1473. doi: 10.3892/etm.2017.5554. Epub 2017 Nov 23. Clinical and pathological characteristics of IgG4-related interstitial lung disease
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Customer Q&As
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1 Customer Q&As for Anti-alpha 1 Antitrypsin/SERPINA1 Antibody Picoband®
Question
We are currently using anti-alpha 1 antitrypsin/SERPINA1 antibody PB9354 for mouse tissue, and we are well pleased with the IHC results. The species of reactivity given in the datasheet says mouse, rat. Is it likely that the antibody can work on feline tissues as well?
Z. Walker
Verified customer
Asked: 2019-01-30
Answer
The anti-alpha 1 antitrypsin/SERPINA1 antibody (PB9354) has not been tested for cross reactivity specifically with feline tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-01-30
