B4GALT1 Antibodies

Beta-1,4-galactosyltransferase 1 (B4GALT1)

The Golgi complex form catalyzes the production of flaxseed from the lactating mammary gland and might also cause the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carbohydrate moieties of glycolipids. .

Gene Information

Human
Gene Name:	B4GALT1
Uniprot:	P15291
Entrez:	2683

Family

Belongs to:
glycosyltransferase 7 family

Alternative Names

1,4- galactosyltransferase, polypeptide 1; B4GalT1; B4GAL-T1; beta-1,4-galactosyltransferase 1; Beta-1,4-GalTase 1; beta4Gal-T1; betaGlcNAc beta; CDG2D; EC 2.4.1; GGTB2; GGTB2DKFZp686N19253; glycoprotein-4-beta-galactosyltransferase 2; GT1; GTB; lactose synthase; MGC50983; UDP-Gal; UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 1; UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 1

Molecular Weight

Mass (kDA):
43.92 kDA

Genomic Context

Human
Location:	9p21.1
Sequence:	9; NC_000009.12 (33104077..33167336, complement)

Tissue Specificity

Ubiquitously expressed, but at very low levels in fetal and adult brain.

Subcellular Localizations

[Isoform Long]: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Cell membrane; Single-pass type II membrane protein. Cell surface. Cell projection, filopodium. Found in trans cisternae of Golgi but is mainly localized at the plasma membrane (PubMed:1714903). B4GALT1 cell surface expression is regulated by UBE2Q1 (By similarity).; [Isoform Short]: Golgi apparatus, Golgi stack membrane; Single-pass type II membrane protein. Found in trans cisternae of Golgi.; [Processed beta-1,4-galactosyltransferase 1]: Secreted. Soluble form found in body fluids.

Diseases

• Malignant Neoplasms
• Malignant Neoplasm Of Breast
• Congenital Disorders Of Glycosylation
• Congenital Disorders
• Neoplasm Metastasis
• Carcinoma
• Inflammation
• Liver Diseases
• Adenoma
• Embryonal Carcinoma
• Inflammatory Bowel Diseases
• Neoplasm Invasiveness
• Intestinal Diseases

• Manic
• Neoplasms
• Metastatic Prostate Cancer
• Lupus Erythematosus, Systemic
• Mammary Neoplasms
• Colorectal Cancer
• Colitis

Pathways

• Glycosylation
• Localization
• Germinal Center Formation
• Mature B Cell Differentiation
• Cell Proliferation
• Lactation
• Fertilization
• Somitogenesis
• Sex Determination
• Dosage Compensation
• Fucosylation
• Methylation
• Flight

• Cytokine Production
• Coagulation
• Cell Differentiation
• Mating
• B Cell Differentiation
• T Cell Costimulation
• Rna Interference

PTMs

• Glycosylation

• Methylation

Research Areas

• Lipid and Metabolism

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/B4GALT1

Boster Bio - Best Uses Of The B4GALT1 Marker

Boster Bio, a company making primary antibodies, secondaries antibodies and Picogram sensitivity ELISA tests, is something you've heard of. But what exactly does this company do and how do they work? Boster's products have applications for scientists all around the globe. The website has lots of useful information. Continue reading to find out more. The Old Order Amish carry a "missense” variant the B4GALT1 genes.

Boster Bio

Boster Bio Anti-B4GALT1/Beta1 4 Gal T1Antibody is a highly-specific anti-B4GALT1 antibody. It reacts both with mouse and human samples. Scientists from many disciplines can submit their results and receive product credits. Boster's antibody against B4GALT1 can be found under catalog number A03993.

Research primary and secondary antibodies

Using the same antibody as the primary, it is possible to visualize the cross-species interaction in mouse anti-GFAP antibody and rat Anti-NeuN antibody. The secondary antibody of the same species can outcompete the primary. This means that mouse-anti-GFAP and mouse-anti-NeuN will exhibit different cross-species interactions. A mouse anti GFAP antibody will show bright staining with a lack of astrocytes. However, a secondary mouse anti rat antibody will not.

A primary antibody reacts to the target antibody exactly and produces very little background staining. It recognizes multiple epitopes of the antigen, making them more sensitive and flexible that secondary antibodies. It is ideal for experiments with denatured proteins. Primary antibodies come in crude or purified form, and are used in immunoassays to detect biomarkers. Secondary antibodies are also used in immunofluorescent stains, western blotting and cell imaging.

Cells containing VPS54-KO and GARP-KO were cultured in tissue culture dishes and lysed in 2% SDS for 5 min. Total protein concentration in cell lysates was determined with a BCA protein assay. After lysing, proteins were loaded onto Bio-Rad gradient gels. Genescript eight- to sixteen-percent gradient Gels were transferred onto membranes using a Pierce G2 fast blotter. After transferring the gels onto the membranes, samples were rinsed in PBS. Then, they were blocked in Odyssey blocking solution for 20 minutes. The membranes were then incubated overnight at 4°C with the primary antibodies.

Picogram sensitivity ELISA kits

ELISA MAX (tm) Standard Sets include all the components necessary to perform an ELISA. Each kit contains a 96-well strip plate pre-coated with the capture antibody. These ELISA strips are used to measure very small amounts of serum factors. These ELISA kit contain the recombinant antibody standard and capture-detection antibodies. The standard sets are validated using human serum, plasma, as well as other biologically relevant samples. ELISA MAX(tm) Deluxe Sets include pre-optimized reagents and buffers that you need to perform an ELISA test.

You must follow the protocol in order to perform the assay. The protocol will be specific for the batch/lot that you are using. To ensure the most accurate results you must follow the instructions. The B4GALT1 marker can be used to detect the presence of cancer cells. It is widely expressed. A reputable laboratory can be consulted if you have questions about the activity or gene of a particular protein.

Old Order Amish may have a "missense variant" in the gene B4GALT1.

A new study found that a certain genetic variant is associated with a lower risk for cardiovascular disease. Old Order Amish have a variant of the gene B4GALT1, which codes to a protein that carries the sugar molecule galactose across cell membranes. Researchers at the University of Maryland delivered the mutation using CRISPR/Cas9 tech to mouse models of cardiovascular diseases. The result was a 38% decrease in blood levels low-density lipoprotein cholesterol, and a decline of blood clotting fibrinogen.

The study also found an unusual genetic variant of B4GALT1 in the Old Order Amish. The Amish are the only population to have this mutation. This research is crucial because it opens up the possibility of finding novel treatments for cardiovascular diseases. The findings must be confirmed by other population-based research.

The study found that the variant in gene B4GALT1 was caused by the deletion nonessential nucleic acids sequences. These segments can be intron sequences. B4GALT1 refers to exons 1-6. It can include exons 1, 4, 5, and any combination of them. These authors believe that the variant in the gene is associated with a decrease in Alzheimer's disease risk.

Despite the fact that researchers often use the term "Old Order Amish", some Amish are unaware of the mutation. Scientists have discovered that 12 % of this group has the mutation. This mutation is relatively common among Amish. Some of them have donated genetic material to scientists because of their belief in the "Good Samaritan” principle. The University of Maryland is home to 140,000 human genes.

Other proteins that can be quantified using ELISA

Enzyme-linked immunesorbent assays (ELISAs), are a popular method for the quantification and analysis of a wide range of proteins and small molecules. ELISAs typically require that the target proteins be in soluble forms. This can complicate the process. Although soluble proteins can be quantified with ELISAs, insoluble proteins are more challenging to measure due to their low extraction efficiency and individual solubilization protocols. A sandwich ELISA can still be used to quantify diverse scaffold proteins as well as synaptic membrane protein.

ELISA uses a microplate and an enzyme to detect the protein. It is a convenient and simple method to measure proteins in complex mixtures. Engvall and Perlmann developed the ELISA method in 1971. It allows for the detection and quantification protein samples that have been immobilized in microplates made of polystyrene. The plates passively attach to proteins and antibodies. This makes it possible to distinguish bound and non-bound material as well as determine the concentrations of target proteins.

In addition to quantitative analysis, ELISA can be used to identify and characterize antibodies to specific antigens. Direct ELISA can be used when only one antibody is available from a sample. Direct ELISAs can be used to measure protein concentrations and to determine the specificity. Boguszewska et. al. published this image of an ELISA. You can also obtain the quantitative data using the same test as a Microarray.

ELISA can also help to quantify other proteins. A standard curve is a well-defined list of protein concentrations that is used to determine OD. This information is used to calculate concentrations of target proteins using a line equation. This method has many benefits. It is sensitive and accurate and has a low CV.