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Product Info Summary
| SKU: | A04368 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-ARA70 NCOA4 Antibody
SKU/Catalog Number
A04368
Size
100μl
Form
Liquid
Description
Boster Bio Anti-ARA70 NCOA4 Antibody catalog # A04368. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ARA70 NCOA4 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04368)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from ARA70 . at AA range: 320-400
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A04368 is reactive to NCOA4 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
39 kDa
Calculated molecular weight
69726 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A04368 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using ARA70 Polyclonal antibody.
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Western blot analysis of NCOA4 Antibody. The lane on the right is blocked with the NCOA4 peptide.
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Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using NCOA4 Antibody. The lane on the right is blocked with the NCOA4 peptide.
Protein Target Info & Infographic
Gene/Protein Information For NCOA4 (Source: Uniprot.org, NCBI)
Gene Name
NCOA4
Full Name
Nuclear receptor coactivator 4
Weight
69726 MW
Alternative Names
Nuclear receptor coactivator 4;NCoA-4;Androgen receptor coactivator 70 kDa protein;70 kDa AR-activator;70 kDa androgen receptor coactivator;Androgen receptor-associated protein of 70 kDa;Ret-activating protein ELE1;NCOA4;ARA70, ELE1, RFG; NCOA4 ARA70, ELE1, PTC3, RFG nuclear receptor coactivator 4 nuclear receptor coactivator 4|70 kDa AR-activator|70 kDa androgen receptor coactivator|NCoA-4|RET-activating gene ELE1|androgen receptor-associated protein of 70 kDa|ret fused
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on NCOA4, check out the NCOA4 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for NCOA4: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ARA70 NCOA4 Antibody (A04368)
Hello CJ!
A04368 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Growth Inhibition of a Novel Iron Chelator, DpdtC, against Hepatoma Carcinoma Cell Lines Partly Attributed to Ferritinophagy-Mediated Lysosomal ROS Generation
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Customer Reviews
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Customer Q&As
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12 Customer Q&As for Anti-ARA70 NCOA4 Antibody
Question
Do you have a BSA free version of anti-ARA70 antibody A04368 available?
G. Mangal
Verified customer
Asked: 2020-03-12
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-ARA70 antibody A04368 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-03-12
Question
Will anti-ARA70 antibody A04368 work for WB with thyroid tumor?
Verified Customer
Verified customer
Asked: 2020-02-19
Answer
According to the expression profile of thyroid tumor, NCOA4 is highly expressed in thyroid tumor. So, it is likely that anti-ARA70 antibody A04368 will work for WB with thyroid tumor.
Boster Scientific Support
Answered: 2020-02-19
Question
See below the WB image, lot number and protocol we used for thyroid tumor using anti-ARA70 antibody A04368. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-12-10
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-12-10
Question
Does anti-ARA70 antibody A04368 work on primate WB with mammary cancer prostatic carcinoma?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
Our lab technicians have not validated anti-ARA70 antibody A04368 on primate. You can run a BLAST between primate and the immunogen sequence of anti-ARA70 antibody A04368 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate mammary cancer prostatic carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for thyroid tumor using anti-ARA70 antibody A04368. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-11-12
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-12
Question
Is this A04368 anti-ARA70 antibody reactive to the isotypes of NCOA4?
Verified Customer
Verified customer
Asked: 2019-06-26
Answer
The immunogen of A04368 anti-ARA70 antibody is Synthesized peptide derived from the Internal region of human ARA70. at AA rangle: 320-400. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-26
Question
I was wanting to use your anti-ARA70 antibody for WB for rat thyroid tumor on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for rat thyroid tumor identification?
Verified Customer
Verified customer
Asked: 2019-06-21
Answer
As indicated on the product datasheet, A04368 anti-ARA70 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat thyroid tumor in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-21
Question
you antibody to test anti-ARA70 antibody A04368 on rat thyroid tumor for research purposes, then I may be interested in using anti-ARA70 antibody A04368 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-05-27
Answer
The products we sell, including anti-ARA70 antibody A04368, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-05-27
Question
Can you help my question with product A04368, anti-ARA70 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
D. Banerjee
Verified customer
Asked: 2017-04-19
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A04368 anti-ARA70 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2017-04-19
Question
I see that the anti-ARA70 antibody A04368 works with WB, what is the protocol used to produce the result images on the product page?
O. Miller
Verified customer
Asked: 2017-03-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2017-03-01
Question
Will A04368 anti-ARA70 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Jones
Verified customer
Asked: 2017-01-06
Answer
You can see on the product datasheet, A04368 anti-ARA70 antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-01-06
Question
Is a blocking peptide available for product anti-ARA70 antibody (A04368)?
D. Patel
Verified customer
Asked: 2016-09-16
Answer
We do provide the blocking peptide for product anti-ARA70 antibody (A04368). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2016-09-16
