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Product Info Summary
| SKU: | A03879 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Kallikrein 2/KLK2 Antibody Picoband®
View all Kallikrein 2 Antibodies
SKU/Catalog Number
A03879
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Kallikrein 2/KLK2 Antibody Picoband® catalog # A03879. Tested in ELISA, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Kallikrein 2/KLK2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03879)
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human Kallikrein 2 recombinant protein (Position: I25-P261).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A03879 is reactive to KLK2 in Human
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
21 kDa
Calculated molecular weight
28.671kDa
Background of Kallikrein 2
KLK2 (KALLIKREIN 2), also called GLANDULAR or PROSTATIC, is a protein that in humans is encoded by the KLK2 gene, and is particularly associated with prostatic tissue. The KLK2 is a member of glandular kallikrein gene family that comprises 25 to 30 highly homologous genes that encode specific proteases involved in the processing of biologically active peptides. The KLK2 gene is mapped to 19q13.33.And the KLK2 gene contains 5 exons. An alternative KLK2 transcript, which they call KLK2-linked molecule (KLM), that arises from the use of an alternate donor site within intron 1.KLM shares only the N-terminal 15-amino acid signal peptide with the original KLK2 protein; the mature proteins display no similarity.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A03879 is guaranteed for ELISA, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
ELISA, 0.1-0.5μg/ml
Positive Control
WB: human MCF-7 cell, human COLO-320 cell, human SK-OV-3 cell, human HepG2 cell
IHC: human prostatic cancer tissue
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Kallikrein 2 using anti-Kallikrein 2 antibody (A03879).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human MCF-7 cell lysate,
Lane 2: human COLO-320 cell lysate,
Lane 3: human SK-OV-3 cell lysate,
Lane 4: human HepG2 cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Kallikrein 2 antigen affinity purified polyclonal antibody (Catalog # A03879) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Kallikrein 2 at approximately 21KD. The expected band size for Kallikrein 2 is at 28KD.
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Figure 2. IHC analysis of Kallikrein 2 using anti-Kallikrein 2 antibody (A03879).
Kallikrein 2 was detected in paraffin-embedded section of human prostatic cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Kallikrein 2 Antibody (A03879) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1022) with DAB as the chromogen.
Protein Target Info & Infographic
Gene/Protein Information For KLK2 (Source: Uniprot.org, NCBI)
Gene Name
KLK2
Full Name
Kallikrein-2
Weight
28.671kDa
Superfamily
peptidase S1 family
Alternative Names
Kallikrein-2; Glandular kallikrein-1; hGK-1; Tissue kallikrein-2; KLK2; KLK2 KLK2A2, hGK-1, hK2 kallikrein related peptidase 2 kallikrein-2|glandular kallikrein 2|glandular kallikrein-1|kallikrein 2, prostatic|tissue kallikrein-2
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KLK2, check out the KLK2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KLK2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Kallikrein 2/KLK2 Antibody Picoband® (A03879)
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Customer Q&As
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5 Customer Q&As for Anti-Kallikrein 2/KLK2 Antibody Picoband®
Question
I am interested in to test anti-Kallikrein 2/KLK2 antibody A03879 on human prostate gland for research purposes, then I may be interested in using anti-Kallikrein 2/KLK2 antibody A03879 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-27
Answer
The products we sell, including anti-Kallikrein 2/KLK2 antibody A03879, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-27
Question
Would anti-Kallikrein 2/KLK2 antibody A03879 work on primate IHC with prostate?
Verified Customer
Verified customer
Asked: 2020-02-03
Answer
Our lab technicians have not validated anti-Kallikrein 2/KLK2 antibody A03879 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Kallikrein 2/KLK2 antibody A03879 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate prostate in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-02-03
Question
Would anti-Kallikrein 2/KLK2 antibody A03879 work for ELISA with prostate gland?
Verified Customer
Verified customer
Asked: 2020-01-14
Answer
According to the expression profile of prostate gland, KLK2 is highly expressed in prostate gland. So, it is likely that anti-Kallikrein 2/KLK2 antibody A03879 will work for ELISA with prostate gland.
Boster Scientific Support
Answered: 2020-01-14
Question
Is this A03879 anti-Kallikrein 2/KLK2 antibody reactive to the isotypes of KLK2?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
The immunogen of A03879 anti-Kallikrein 2/KLK2 antibody is E. coli-derived human Kallikrein 2 recombinant protein (Position: I25-P261). Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-06-05
Question
I see that the anti-Kallikrein 2/KLK2 antibody A03879 works with ELISA, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-11-20
Answer
You can find protocols for ELISA on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2018-11-20
